Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli.
Entamoeba nuttalli is an intestinal protozoan with pathogenic potential that can cause amebic liver abscess. It is highly prevalent in wild and captive macaques. Recently, cysts were detected in a caretaker of nonhuman primates in a zoo, indicating that E. nuttalli may be a zoonotic pathogen. Theref...
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ftdoajarticles:oai:doaj.org/article:5631438054f64aa2af07bc98ac713d67 2023-05-15T15:12:09+02:00 Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. Yue Guan Meng Feng Xiangyang Min Hang Zhou Yongfeng Fu Hiroshi Tachibana Xunjia Cheng 2018-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006216 https://doaj.org/article/5631438054f64aa2af07bc98ac713d67 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5821383?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006216 https://doaj.org/article/5631438054f64aa2af07bc98ac713d67 PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006216 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006216 2022-12-31T01:01:25Z Entamoeba nuttalli is an intestinal protozoan with pathogenic potential that can cause amebic liver abscess. It is highly prevalent in wild and captive macaques. Recently, cysts were detected in a caretaker of nonhuman primates in a zoo, indicating that E. nuttalli may be a zoonotic pathogen. Therefore, it is important to evaluate the pathogenicity of E. nuttalli in detail and in comparison with that of E. histolytica.Trophozoites of E. nuttalli GY4 and E. histolytica SAW755 strains were inoculated into liver of hamsters. Expression levels of proinflammatory factors of hamsters and virulence factors from E. histolytica and E. nuttalli were compared between the two parasites. Inoculations with trophozoites of E. nuttalli resulted in an average necrotic area of 24% in liver tissue in 7 days, whereas this area produced by E. histolytica was nearly 50%. Along with the mild liver tissue damage induced by E. nuttalli, expression levels of proinflammatory factors (TNF-α, IL-6 and IL-1β) and amebic virulence protein genes (lectins, cysteine proteases and amoeba pores) in local tissues were lower with E. nuttalli in comparison with E. histolytica. In addition, M2 type macrophages were increased in E. nuttalli-induced amebic liver abscesses in the late stage of disease progression and lysate of E. nuttalli trophozoites induced higher arginase expression than E. histolytica in vitro.The results show that differential secretion of amebic virulence proteins during E. nuttalli infection triggered lower levels of secretion of various cytokines and had an impact on polarization of macrophages towards a M1/M2 balance. However, regardless of the degree of macrophage polarization, there is unambiguous evidence of an intense acute inflammatory reaction in liver of hamsters after infection by both Entamoeba species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 2 e0006216 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yue Guan Meng Feng Xiangyang Min Hang Zhou Yongfeng Fu Hiroshi Tachibana Xunjia Cheng Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Entamoeba nuttalli is an intestinal protozoan with pathogenic potential that can cause amebic liver abscess. It is highly prevalent in wild and captive macaques. Recently, cysts were detected in a caretaker of nonhuman primates in a zoo, indicating that E. nuttalli may be a zoonotic pathogen. Therefore, it is important to evaluate the pathogenicity of E. nuttalli in detail and in comparison with that of E. histolytica.Trophozoites of E. nuttalli GY4 and E. histolytica SAW755 strains were inoculated into liver of hamsters. Expression levels of proinflammatory factors of hamsters and virulence factors from E. histolytica and E. nuttalli were compared between the two parasites. Inoculations with trophozoites of E. nuttalli resulted in an average necrotic area of 24% in liver tissue in 7 days, whereas this area produced by E. histolytica was nearly 50%. Along with the mild liver tissue damage induced by E. nuttalli, expression levels of proinflammatory factors (TNF-α, IL-6 and IL-1β) and amebic virulence protein genes (lectins, cysteine proteases and amoeba pores) in local tissues were lower with E. nuttalli in comparison with E. histolytica. In addition, M2 type macrophages were increased in E. nuttalli-induced amebic liver abscesses in the late stage of disease progression and lysate of E. nuttalli trophozoites induced higher arginase expression than E. histolytica in vitro.The results show that differential secretion of amebic virulence proteins during E. nuttalli infection triggered lower levels of secretion of various cytokines and had an impact on polarization of macrophages towards a M1/M2 balance. However, regardless of the degree of macrophage polarization, there is unambiguous evidence of an intense acute inflammatory reaction in liver of hamsters after infection by both Entamoeba species. |
format |
Article in Journal/Newspaper |
author |
Yue Guan Meng Feng Xiangyang Min Hang Zhou Yongfeng Fu Hiroshi Tachibana Xunjia Cheng |
author_facet |
Yue Guan Meng Feng Xiangyang Min Hang Zhou Yongfeng Fu Hiroshi Tachibana Xunjia Cheng |
author_sort |
Yue Guan |
title |
Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
title_short |
Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
title_full |
Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
title_fullStr |
Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
title_full_unstemmed |
Characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with Entamoeba nuttalli. |
title_sort |
characteristics of inflammatory reactions during development of liver abscess in hamsters inoculated with entamoeba nuttalli. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006216 https://doaj.org/article/5631438054f64aa2af07bc98ac713d67 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006216 (2018) |
op_relation |
http://europepmc.org/articles/PMC5821383?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006216 https://doaj.org/article/5631438054f64aa2af07bc98ac713d67 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006216 |
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PLOS Neglected Tropical Diseases |
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12 |
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2 |
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e0006216 |
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