Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method
Objective: To perform aqueous ethanol soluble fraction (AESF) and dichloromethane extract of aerial parts of Maytenus obscura (A. Rich.) Cuf. using high performance thin layer chromatography (HPTLC) and to test anti-inflammatory activity of these extracts. Methods: HPTLC studies were carried out usi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2014
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Online Access: | https://doi.org/10.1016/S2221-1691(14)60224-0 https://doaj.org/article/55eee467b55f4e5a86314c139bd0bc35 |
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ftdoajarticles:oai:doaj.org/article:55eee467b55f4e5a86314c139bd0bc35 2023-05-15T15:17:13+02:00 Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method Mohamed F. Alajmi Perwez Alam 2014-02-01T00:00:00Z https://doi.org/10.1016/S2221-1691(14)60224-0 https://doaj.org/article/55eee467b55f4e5a86314c139bd0bc35 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301854 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60224-0 https://doaj.org/article/55eee467b55f4e5a86314c139bd0bc35 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 2, Pp 152-157 (2014) Maytenus obscura Phytochemical screening Finger print Anti-inflammatory HPTLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.1016/S2221-1691(14)60224-0 2022-12-31T01:33:34Z Objective: To perform aqueous ethanol soluble fraction (AESF) and dichloromethane extract of aerial parts of Maytenus obscura (A. Rich.) Cuf. using high performance thin layer chromatography (HPTLC) and to test anti-inflammatory activity of these extracts. Methods: HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software were used. The anti-inflammatory activity was tested by injecting different groups of rats (6 each) with formalin in hind paw and measuring the edema volume before and 1 h later formalin injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 100 and 200 mg/kg 1 h before formalin administration. Indomethacin (30 mg/kg) was used as standard. Results: The results of preliminary phytochemical studies confirmed the presence of protein, lipid, carbohydrate, phenol, flavonoid, saponin, triterpenoid, alkaloid and anthraquinone in both extracts. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene: ethyacetate: glacial acetic acid (5:3:0.2, v/v/v) as mobile phase. HPTLC finger printing of AESF revealed major eight peaks with Rf values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with Rf values in the range of 0.12 to 0.76. The purity of sample was confirmed by comparing the absorption spectra at start, middle and end position of the band. Treatment of rats (i.p.) with AESF and dichloromethane in doses of 100 and 200 mg/kg inhibited singnificantly (P<0.05, n=6) formalin-induced inflammation by 50%, 55.9%, 45.5%, and 51.4%, respectively. Conclusions: HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts. These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts. The anti-inflammatory activity of both ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 2 152 157 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Maytenus obscura Phytochemical screening Finger print Anti-inflammatory HPTLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Maytenus obscura Phytochemical screening Finger print Anti-inflammatory HPTLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mohamed F. Alajmi Perwez Alam Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
topic_facet |
Maytenus obscura Phytochemical screening Finger print Anti-inflammatory HPTLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To perform aqueous ethanol soluble fraction (AESF) and dichloromethane extract of aerial parts of Maytenus obscura (A. Rich.) Cuf. using high performance thin layer chromatography (HPTLC) and to test anti-inflammatory activity of these extracts. Methods: HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator, TLC scanner 3, Reprostar 3, CAMAG ADC 2 and WIN CATS-4 software were used. The anti-inflammatory activity was tested by injecting different groups of rats (6 each) with formalin in hind paw and measuring the edema volume before and 1 h later formalin injection. Control group received saline i.p. The extracts treatment was injected i.p. in doses of 100 and 200 mg/kg 1 h before formalin administration. Indomethacin (30 mg/kg) was used as standard. Results: The results of preliminary phytochemical studies confirmed the presence of protein, lipid, carbohydrate, phenol, flavonoid, saponin, triterpenoid, alkaloid and anthraquinone in both extracts. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene: ethyacetate: glacial acetic acid (5:3:0.2, v/v/v) as mobile phase. HPTLC finger printing of AESF revealed major eight peaks with Rf values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with Rf values in the range of 0.12 to 0.76. The purity of sample was confirmed by comparing the absorption spectra at start, middle and end position of the band. Treatment of rats (i.p.) with AESF and dichloromethane in doses of 100 and 200 mg/kg inhibited singnificantly (P<0.05, n=6) formalin-induced inflammation by 50%, 55.9%, 45.5%, and 51.4%, respectively. Conclusions: HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts. These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts. The anti-inflammatory activity of both ... |
format |
Article in Journal/Newspaper |
author |
Mohamed F. Alajmi Perwez Alam |
author_facet |
Mohamed F. Alajmi Perwez Alam |
author_sort |
Mohamed F. Alajmi |
title |
Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
title_short |
Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
title_full |
Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
title_fullStr |
Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
title_full_unstemmed |
Anti–inflammatory activity and qualitative analysis of different extracts of Maytenus obscura (A. Rich.) Cuf. by high performance thin layer chromatography method |
title_sort |
anti–inflammatory activity and qualitative analysis of different extracts of maytenus obscura (a. rich.) cuf. by high performance thin layer chromatography method |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.1016/S2221-1691(14)60224-0 https://doaj.org/article/55eee467b55f4e5a86314c139bd0bc35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 2, Pp 152-157 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301854 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60224-0 https://doaj.org/article/55eee467b55f4e5a86314c139bd0bc35 |
op_doi |
https://doi.org/10.1016/S2221-1691(14)60224-0 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
2 |
container_start_page |
152 |
op_container_end_page |
157 |
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1766347484300312576 |