Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol.
BACKGROUND:To work towards reaching the WHO goal of eliminating soil-transmitted helminth (STH) infections as a public health problem, the total number of children receiving anthelmintic drugs has strongly increased over the past few years. However, as drug pressure levels rise, the development of a...
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ftdoajarticles:oai:doaj.org/article:55d95437fab94114a09a028693c537af 2023-05-15T15:17:24+02:00 Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. Johnny Vlaminck Piet Cools Marco Albonico Shaali Ame Mio Ayana Jeffrey Bethony Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Roger Prichard Nour Rashwan Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke 2018-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006912 https://doaj.org/article/55d95437fab94114a09a028693c537af EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6235403?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006912 https://doaj.org/article/55d95437fab94114a09a028693c537af PLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006912 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006912 2022-12-31T15:07:50Z BACKGROUND:To work towards reaching the WHO goal of eliminating soil-transmitted helminth (STH) infections as a public health problem, the total number of children receiving anthelmintic drugs has strongly increased over the past few years. However, as drug pressure levels rise, the development of anthelmintic drug resistance (AR) is more and more likely to appear. Currently, any global surveillance system to monitor drug efficacy and the emergence of possible AR is lacking. Consequently, it remains unclear to what extent the efficacy of drugs may have dropped and whether AR is already present. The overall aim of this study is to recommend the best diagnostic methods to monitor drug efficacy and molecular markers to assess the emergence of AR in STH control programs. METHODS:A series of drug efficacy trials will be performed in four STH endemic countries with varying drug pressure (Ethiopia and Brazil: low drug pressure, Lao PDR: moderate drug pressure and Tanzania: high drug pressure). These trials are designed to assess the efficacy of a single oral dose of 400 mg albendazole (ALB) against STH infections in school-aged children (SAC) by microscopic (duplicate Kato-Katz thick smear, Mini-FLOTAC and FECPAKG2) and molecular stool-based diagnostic methods (quantitative PCR (qPCR)). Data will be collected on the cost of the materials used, as well as the time required to prepare and examine stool samples for the different diagnostic methods. Following qPCR, DNA samples will also be submitted for pyrosequencing to assess the presence and prevalence of single nucleotide polymorphisms (SNPs) in the β-tubulin gene. These SNPs are known to be linked to AR in animal STHs. DISCUSSION:The results obtained by these trials will provide robust evidence regarding the cost-efficiency and diagnostic performance of the different stool-based diagnostic methods for the assessment of drug efficacy in control programs. The assessment of associations between the frequency of SNPs in the β-tubulin gene and the history of drug pressure ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 11 e0006912 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Johnny Vlaminck Piet Cools Marco Albonico Shaali Ame Mio Ayana Jeffrey Bethony Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Roger Prichard Nour Rashwan Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:To work towards reaching the WHO goal of eliminating soil-transmitted helminth (STH) infections as a public health problem, the total number of children receiving anthelmintic drugs has strongly increased over the past few years. However, as drug pressure levels rise, the development of anthelmintic drug resistance (AR) is more and more likely to appear. Currently, any global surveillance system to monitor drug efficacy and the emergence of possible AR is lacking. Consequently, it remains unclear to what extent the efficacy of drugs may have dropped and whether AR is already present. The overall aim of this study is to recommend the best diagnostic methods to monitor drug efficacy and molecular markers to assess the emergence of AR in STH control programs. METHODS:A series of drug efficacy trials will be performed in four STH endemic countries with varying drug pressure (Ethiopia and Brazil: low drug pressure, Lao PDR: moderate drug pressure and Tanzania: high drug pressure). These trials are designed to assess the efficacy of a single oral dose of 400 mg albendazole (ALB) against STH infections in school-aged children (SAC) by microscopic (duplicate Kato-Katz thick smear, Mini-FLOTAC and FECPAKG2) and molecular stool-based diagnostic methods (quantitative PCR (qPCR)). Data will be collected on the cost of the materials used, as well as the time required to prepare and examine stool samples for the different diagnostic methods. Following qPCR, DNA samples will also be submitted for pyrosequencing to assess the presence and prevalence of single nucleotide polymorphisms (SNPs) in the β-tubulin gene. These SNPs are known to be linked to AR in animal STHs. DISCUSSION:The results obtained by these trials will provide robust evidence regarding the cost-efficiency and diagnostic performance of the different stool-based diagnostic methods for the assessment of drug efficacy in control programs. The assessment of associations between the frequency of SNPs in the β-tubulin gene and the history of drug pressure ... |
format |
Article in Journal/Newspaper |
author |
Johnny Vlaminck Piet Cools Marco Albonico Shaali Ame Mio Ayana Jeffrey Bethony Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Roger Prichard Nour Rashwan Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke |
author_facet |
Johnny Vlaminck Piet Cools Marco Albonico Shaali Ame Mio Ayana Jeffrey Bethony Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Roger Prichard Nour Rashwan Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke |
author_sort |
Johnny Vlaminck |
title |
Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
title_short |
Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
title_full |
Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
title_fullStr |
Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
title_full_unstemmed |
Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol. |
title_sort |
comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: a starworms study protocol. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006912 https://doaj.org/article/55d95437fab94114a09a028693c537af |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006912 (2018) |
op_relation |
http://europepmc.org/articles/PMC6235403?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006912 https://doaj.org/article/55d95437fab94114a09a028693c537af |
op_doi |
https://doi.org/10.1371/journal.pntd.0006912 |
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PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
11 |
container_start_page |
e0006912 |
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1766347651100442624 |