A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus.
The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected pati...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:55c613956c1247759a0027ccc56b6df1 2023-05-15T15:05:03+02:00 A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. Diogo M Magnani Cassia G T Silveira Brandon C Rosen Michael J Ricciardi Núria Pedreño-Lopez Martin J Gutman Varian K Bailey Helen S Maxwell Aline Domingues Lucas Gonzalez-Nieto Vivian I Avelino-Silva Mateus Trindade Juliana Nogueira Consuelo S Oliveira Alvino Maestri Alvina Clara Felix José Eduardo Levi Mauricio L Nogueira Mauricio A Martins José M Martinez-Navio Sebastian P Fuchs Stephen S Whitehead Dennis R Burton Ronald C Desrosiers Esper G Kallas David I Watkins 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005655 https://doaj.org/article/55c613956c1247759a0027ccc56b6df1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5481143?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005655 https://doaj.org/article/55c613956c1247759a0027ccc56b6df1 PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005655 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005655 2022-12-31T12:03:25Z The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut50 ~ 2 μg/ml) but did not react with any of the four dengue virus serotypes. Except for the expected junctional diversity created by the joining of the V-(D)-J genes, there was no deviation from immunoglobulin germline genes. This is a rare example of a human mAb with neutralizing activity in the absence of detectable somatic hypermutation. Importantly, binding of this mAb to ZIKV was specifically inhibited by human plasma from ZIKV-exposed individuals, suggesting that it may be of value in a diagnostic setting. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 6 e0005655 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Diogo M Magnani Cassia G T Silveira Brandon C Rosen Michael J Ricciardi Núria Pedreño-Lopez Martin J Gutman Varian K Bailey Helen S Maxwell Aline Domingues Lucas Gonzalez-Nieto Vivian I Avelino-Silva Mateus Trindade Juliana Nogueira Consuelo S Oliveira Alvino Maestri Alvina Clara Felix José Eduardo Levi Mauricio L Nogueira Mauricio A Martins José M Martinez-Navio Sebastian P Fuchs Stephen S Whitehead Dennis R Burton Ronald C Desrosiers Esper G Kallas David I Watkins A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut50 ~ 2 μg/ml) but did not react with any of the four dengue virus serotypes. Except for the expected junctional diversity created by the joining of the V-(D)-J genes, there was no deviation from immunoglobulin germline genes. This is a rare example of a human mAb with neutralizing activity in the absence of detectable somatic hypermutation. Importantly, binding of this mAb to ZIKV was specifically inhibited by human plasma from ZIKV-exposed individuals, suggesting that it may be of value in a diagnostic setting. |
format |
Article in Journal/Newspaper |
author |
Diogo M Magnani Cassia G T Silveira Brandon C Rosen Michael J Ricciardi Núria Pedreño-Lopez Martin J Gutman Varian K Bailey Helen S Maxwell Aline Domingues Lucas Gonzalez-Nieto Vivian I Avelino-Silva Mateus Trindade Juliana Nogueira Consuelo S Oliveira Alvino Maestri Alvina Clara Felix José Eduardo Levi Mauricio L Nogueira Mauricio A Martins José M Martinez-Navio Sebastian P Fuchs Stephen S Whitehead Dennis R Burton Ronald C Desrosiers Esper G Kallas David I Watkins |
author_facet |
Diogo M Magnani Cassia G T Silveira Brandon C Rosen Michael J Ricciardi Núria Pedreño-Lopez Martin J Gutman Varian K Bailey Helen S Maxwell Aline Domingues Lucas Gonzalez-Nieto Vivian I Avelino-Silva Mateus Trindade Juliana Nogueira Consuelo S Oliveira Alvino Maestri Alvina Clara Felix José Eduardo Levi Mauricio L Nogueira Mauricio A Martins José M Martinez-Navio Sebastian P Fuchs Stephen S Whitehead Dennis R Burton Ronald C Desrosiers Esper G Kallas David I Watkins |
author_sort |
Diogo M Magnani |
title |
A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
title_short |
A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
title_full |
A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
title_fullStr |
A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
title_full_unstemmed |
A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. |
title_sort |
human inferred germline antibody binds to an immunodominant epitope and neutralizes zika virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005655 https://doaj.org/article/55c613956c1247759a0027ccc56b6df1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005655 (2017) |
op_relation |
http://europepmc.org/articles/PMC5481143?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005655 https://doaj.org/article/55c613956c1247759a0027ccc56b6df1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005655 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
6 |
container_start_page |
e0005655 |
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1766336811064360960 |