Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda.
Tsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glossina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense dis...
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ftdoajarticles:oai:doaj.org/article:55c1587c164a4b1189150e3cc75ea56a 2023-05-15T15:14:17+02:00 Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. Daniela I Schneider Norah Saarman Maria G Onyango Chaz Hyseni Robert Opiro Richard Echodu Michelle O'Neill Danielle Bloch Aurélien Vigneron T J Johnson Kirstin Dion Brian L Weiss Elizabeth Opiyo Adalgisa Caccone Serap Aksoy 2019-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007340 https://doaj.org/article/55c1587c164a4b1189150e3cc75ea56a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007340 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007340 https://doaj.org/article/55c1587c164a4b1189150e3cc75ea56a PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007340 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007340 2022-12-31T05:48:39Z Tsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glossina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions. Endosymbionts can influence transmission efficiency of parasites through their insect vectors via conferring a protective effect against the parasite. It is known that the bacterium Spiroplasma is capable of protecting its Drosophila host from infection with a parasitic nematode. This endosymbiont can also impact its host's population structure via altering host reproductive traits. Here, we used field collections across 26 different Gff sampling sites in northern and western Uganda to investigate the association of Spiroplasma with geographic origin, seasonal conditions, Gff genetic background and sex, and trypanosome infection status. We also investigated the influence of Spiroplasma on Gff vector competence to trypanosome infections under laboratory conditions. Generalized linear models (GLM) showed that Spiroplasma probability was correlated with the geographic origin of Gff host and with the season of collection, with higher prevalence found in flies within the Albert Nile (0.42 vs 0.16) and Achwa River (0.36 vs 0.08) watersheds and with higher prevalence detected in flies collected in the intermediate than wet season. In contrast, there was no significant correlation of Spiroplasma prevalence with Gff host genetic background or sex once geographic origin was accounted for in generalized linear models. Additionally, we found a potential negative correlation of Spiroplasma with trypanosome infection, with only 2% of Spiroplasma infected flies harboring trypanosome co-infections. We also found that in a laboratory line of Gff, parasitic trypanosomes are less likely to colonize the midgut in individuals that harbor Spiroplasma infection. These results indicate that ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 8 e0007340 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniela I Schneider Norah Saarman Maria G Onyango Chaz Hyseni Robert Opiro Richard Echodu Michelle O'Neill Danielle Bloch Aurélien Vigneron T J Johnson Kirstin Dion Brian L Weiss Elizabeth Opiyo Adalgisa Caccone Serap Aksoy Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Tsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glossina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions. Endosymbionts can influence transmission efficiency of parasites through their insect vectors via conferring a protective effect against the parasite. It is known that the bacterium Spiroplasma is capable of protecting its Drosophila host from infection with a parasitic nematode. This endosymbiont can also impact its host's population structure via altering host reproductive traits. Here, we used field collections across 26 different Gff sampling sites in northern and western Uganda to investigate the association of Spiroplasma with geographic origin, seasonal conditions, Gff genetic background and sex, and trypanosome infection status. We also investigated the influence of Spiroplasma on Gff vector competence to trypanosome infections under laboratory conditions. Generalized linear models (GLM) showed that Spiroplasma probability was correlated with the geographic origin of Gff host and with the season of collection, with higher prevalence found in flies within the Albert Nile (0.42 vs 0.16) and Achwa River (0.36 vs 0.08) watersheds and with higher prevalence detected in flies collected in the intermediate than wet season. In contrast, there was no significant correlation of Spiroplasma prevalence with Gff host genetic background or sex once geographic origin was accounted for in generalized linear models. Additionally, we found a potential negative correlation of Spiroplasma with trypanosome infection, with only 2% of Spiroplasma infected flies harboring trypanosome co-infections. We also found that in a laboratory line of Gff, parasitic trypanosomes are less likely to colonize the midgut in individuals that harbor Spiroplasma infection. These results indicate that ... |
format |
Article in Journal/Newspaper |
author |
Daniela I Schneider Norah Saarman Maria G Onyango Chaz Hyseni Robert Opiro Richard Echodu Michelle O'Neill Danielle Bloch Aurélien Vigneron T J Johnson Kirstin Dion Brian L Weiss Elizabeth Opiyo Adalgisa Caccone Serap Aksoy |
author_facet |
Daniela I Schneider Norah Saarman Maria G Onyango Chaz Hyseni Robert Opiro Richard Echodu Michelle O'Neill Danielle Bloch Aurélien Vigneron T J Johnson Kirstin Dion Brian L Weiss Elizabeth Opiyo Adalgisa Caccone Serap Aksoy |
author_sort |
Daniela I Schneider |
title |
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
title_short |
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
title_full |
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
title_fullStr |
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
title_full_unstemmed |
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. |
title_sort |
spatio-temporal distribution of spiroplasma infections in the tsetse fly (glossina fuscipes fuscipes) in northern uganda. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007340 https://doaj.org/article/55c1587c164a4b1189150e3cc75ea56a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007340 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007340 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007340 https://doaj.org/article/55c1587c164a4b1189150e3cc75ea56a |
op_doi |
https://doi.org/10.1371/journal.pntd.0007340 |
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PLOS Neglected Tropical Diseases |
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13 |
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8 |
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e0007340 |
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1766344749354057728 |