Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis.
This study reports on in vitro and in vivo tests that sought to assess the antifungal activity of a newly developed magnetic carrier system comprising amphotericin B loaded onto the surface of pre-coated (with a double-layer of lauric acid) magnetite nanoparticles. The in vitro tests compared two dr...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:55959114648844a795b616fa8d8e2690 2023-05-15T15:08:10+02:00 Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. Camila Arruda Saldanha Mônica Pereira Garcia Diego Cesar Iocca Luciana Guilherme Rebelo Ana Camila Oliveira Souza Anamélia Lorenzetti Bocca Maria de Fátima Menezes Almeida Santos Paulo Cesar Morais Ricardo Bentes Azevedo 2016-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004754 https://doaj.org/article/55959114648844a795b616fa8d8e2690 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4909273?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004754 https://doaj.org/article/55959114648844a795b616fa8d8e2690 PLoS Neglected Tropical Diseases, Vol 10, Iss 6, p e0004754 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004754 2022-12-31T13:04:09Z This study reports on in vitro and in vivo tests that sought to assess the antifungal activity of a newly developed magnetic carrier system comprising amphotericin B loaded onto the surface of pre-coated (with a double-layer of lauric acid) magnetite nanoparticles. The in vitro tests compared two drugs; i.e., this newly developed form and free amphotericin B. We found that this nanocomplex exhibited antifungal activity without cytotoxicity to human urinary cells and with low cytotoxicity to peritoneal macrophages. We also evaluated the efficacy of the nanocomplex in experimental paracoccidioidomycosis. BALB/c mice were intratracheally infected with Paracoccidioides brasiliensis and treated with the compound for 30 or 60 days beginning the day after infection. The newly developed amphotericin B coupled with magnetic nanoparticles was effective against experimental paracoccidioidomycosis, and it did not induce clinical, biochemical or histopathological alterations. The nanocomplex also did not induce genotoxic effects in bone marrow cells. Therefore, it is reasonable to believe that amphotericin B coupled to magnetic nanoparticles and stabilized with bilayer lauric acid is a promising nanotool for the treatment of the experimental paracoccidioidomycosis because it exhibited antifungal activity that was similar to that of free amphotericin B, did not induce adverse effects in therapeutic doses and allowed for a reduction in the number of applications. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 6 e0004754 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Camila Arruda Saldanha Mônica Pereira Garcia Diego Cesar Iocca Luciana Guilherme Rebelo Ana Camila Oliveira Souza Anamélia Lorenzetti Bocca Maria de Fátima Menezes Almeida Santos Paulo Cesar Morais Ricardo Bentes Azevedo Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
This study reports on in vitro and in vivo tests that sought to assess the antifungal activity of a newly developed magnetic carrier system comprising amphotericin B loaded onto the surface of pre-coated (with a double-layer of lauric acid) magnetite nanoparticles. The in vitro tests compared two drugs; i.e., this newly developed form and free amphotericin B. We found that this nanocomplex exhibited antifungal activity without cytotoxicity to human urinary cells and with low cytotoxicity to peritoneal macrophages. We also evaluated the efficacy of the nanocomplex in experimental paracoccidioidomycosis. BALB/c mice were intratracheally infected with Paracoccidioides brasiliensis and treated with the compound for 30 or 60 days beginning the day after infection. The newly developed amphotericin B coupled with magnetic nanoparticles was effective against experimental paracoccidioidomycosis, and it did not induce clinical, biochemical or histopathological alterations. The nanocomplex also did not induce genotoxic effects in bone marrow cells. Therefore, it is reasonable to believe that amphotericin B coupled to magnetic nanoparticles and stabilized with bilayer lauric acid is a promising nanotool for the treatment of the experimental paracoccidioidomycosis because it exhibited antifungal activity that was similar to that of free amphotericin B, did not induce adverse effects in therapeutic doses and allowed for a reduction in the number of applications. |
format |
Article in Journal/Newspaper |
author |
Camila Arruda Saldanha Mônica Pereira Garcia Diego Cesar Iocca Luciana Guilherme Rebelo Ana Camila Oliveira Souza Anamélia Lorenzetti Bocca Maria de Fátima Menezes Almeida Santos Paulo Cesar Morais Ricardo Bentes Azevedo |
author_facet |
Camila Arruda Saldanha Mônica Pereira Garcia Diego Cesar Iocca Luciana Guilherme Rebelo Ana Camila Oliveira Souza Anamélia Lorenzetti Bocca Maria de Fátima Menezes Almeida Santos Paulo Cesar Morais Ricardo Bentes Azevedo |
author_sort |
Camila Arruda Saldanha |
title |
Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
title_short |
Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
title_full |
Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
title_fullStr |
Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
title_full_unstemmed |
Antifungal Activity of Amphotericin B Conjugated to Nanosized Magnetite in the Treatment of Paracoccidioidomycosis. |
title_sort |
antifungal activity of amphotericin b conjugated to nanosized magnetite in the treatment of paracoccidioidomycosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004754 https://doaj.org/article/55959114648844a795b616fa8d8e2690 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 6, p e0004754 (2016) |
op_relation |
http://europepmc.org/articles/PMC4909273?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004754 https://doaj.org/article/55959114648844a795b616fa8d8e2690 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004754 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
6 |
container_start_page |
e0004754 |
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1766339579817754624 |