Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts
For full use of the by-products during Siberian sturgeon (Acipenser baerii) processing, gelatin was extracted from the cartilages using the hot water method, and its physico-chemical properties and protective function on ultraviolet-A injured human skin fibroblasts (HFSBs) were measured. Using singl...
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ftdoajarticles:oai:doaj.org/article:557d1f972d884b6ab5dab18233b14805 2023-05-15T13:01:40+02:00 Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts Zhe Zhang Yu-Mei Wang Yi-Ting Qiu Chang-Feng Chi Hong-Yu Luo Bin Wang 2022-06-01T00:00:00Z https://doi.org/10.3389/fmars.2022.925407 https://doaj.org/article/557d1f972d884b6ab5dab18233b14805 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.925407/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.925407 https://doaj.org/article/557d1f972d884b6ab5dab18233b14805 Frontiers in Marine Science, Vol 9 (2022) Siberian sturgeon (Acipenser baerii) cartilage gelatin cytoprotection ultraviolet-A injury Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.925407 2022-12-31T02:31:40Z For full use of the by-products during Siberian sturgeon (Acipenser baerii) processing, gelatin was extracted from the cartilages using the hot water method, and its physico-chemical properties and protective function on ultraviolet-A injured human skin fibroblasts (HFSBs) were measured. Using single-factor and orthogonal experiments, the conditions for extracting gelatin from Siberian sturgeon cartilage were optimized as extraction time of 7 h, pH 9, material-to-liquid ratio (g/ml) of 1:5, and temperature of 45°C. The prepared gelatin (TCG) with a yield of 28.8 ± 1.53% had Gly (307 residues/1,000 residues) as the major amino acid and contained a lower amount (214 residues/1,000 residues) of imino acids than that (227 residues/1,000 residues) of pigskin gelatin (PSG). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet spectrum, and infrared spectroscopy analysis indicated that TCG had the main spectral characteristics of fish gelatin and contained α chains (α1 and α2 chains) and β chain of type I collagen, but its structural stability was lower than that of PSG due to its low content of imino acids, which induced the smaller molecular bands observed in the SDS-PAGE pattern. TCG exhibited lower water content, gel strength, emulsion stability index, foam capacity, foam stability, and water-holding capacity but higher ash content, transmittance, emulsion activity index, and fat-binding capacity (P < 0.05). Moreover, TCG could significantly protect HFSBs against ultraviolet-A injury by enhancing the activity of superoxide dismutase, catalase, and glutathione peroxidase to scavenge excess reactive oxygen species and decrease the content of malondialdehyde. Therefore, gelatin from Siberian sturgeon cartilages could act as promising candidates when applied in health-promoting products against ultraviolet-A injury. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Siberian sturgeon (Acipenser baerii) cartilage gelatin cytoprotection ultraviolet-A injury Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Siberian sturgeon (Acipenser baerii) cartilage gelatin cytoprotection ultraviolet-A injury Science Q General. Including nature conservation geographical distribution QH1-199.5 Zhe Zhang Yu-Mei Wang Yi-Ting Qiu Chang-Feng Chi Hong-Yu Luo Bin Wang Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
topic_facet |
Siberian sturgeon (Acipenser baerii) cartilage gelatin cytoprotection ultraviolet-A injury Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
For full use of the by-products during Siberian sturgeon (Acipenser baerii) processing, gelatin was extracted from the cartilages using the hot water method, and its physico-chemical properties and protective function on ultraviolet-A injured human skin fibroblasts (HFSBs) were measured. Using single-factor and orthogonal experiments, the conditions for extracting gelatin from Siberian sturgeon cartilage were optimized as extraction time of 7 h, pH 9, material-to-liquid ratio (g/ml) of 1:5, and temperature of 45°C. The prepared gelatin (TCG) with a yield of 28.8 ± 1.53% had Gly (307 residues/1,000 residues) as the major amino acid and contained a lower amount (214 residues/1,000 residues) of imino acids than that (227 residues/1,000 residues) of pigskin gelatin (PSG). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet spectrum, and infrared spectroscopy analysis indicated that TCG had the main spectral characteristics of fish gelatin and contained α chains (α1 and α2 chains) and β chain of type I collagen, but its structural stability was lower than that of PSG due to its low content of imino acids, which induced the smaller molecular bands observed in the SDS-PAGE pattern. TCG exhibited lower water content, gel strength, emulsion stability index, foam capacity, foam stability, and water-holding capacity but higher ash content, transmittance, emulsion activity index, and fat-binding capacity (P < 0.05). Moreover, TCG could significantly protect HFSBs against ultraviolet-A injury by enhancing the activity of superoxide dismutase, catalase, and glutathione peroxidase to scavenge excess reactive oxygen species and decrease the content of malondialdehyde. Therefore, gelatin from Siberian sturgeon cartilages could act as promising candidates when applied in health-promoting products against ultraviolet-A injury. |
format |
Article in Journal/Newspaper |
author |
Zhe Zhang Yu-Mei Wang Yi-Ting Qiu Chang-Feng Chi Hong-Yu Luo Bin Wang |
author_facet |
Zhe Zhang Yu-Mei Wang Yi-Ting Qiu Chang-Feng Chi Hong-Yu Luo Bin Wang |
author_sort |
Zhe Zhang |
title |
Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
title_short |
Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
title_full |
Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
title_fullStr |
Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
title_full_unstemmed |
Gelatin From Cartilage of Siberian Sturgeon (Acipenser baerii): Preparation, Characterization, and Protective Function on Ultraviolet-A-Injured Human Skin Fibroblasts |
title_sort |
gelatin from cartilage of siberian sturgeon (acipenser baerii): preparation, characterization, and protective function on ultraviolet-a-injured human skin fibroblasts |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.925407 https://doaj.org/article/557d1f972d884b6ab5dab18233b14805 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.925407/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.925407 https://doaj.org/article/557d1f972d884b6ab5dab18233b14805 |
op_doi |
https://doi.org/10.3389/fmars.2022.925407 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
_version_ |
1766283564350963712 |