Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content.
Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the...
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ftdoajarticles:oai:doaj.org/article:556095a013fd40e1b551ce787e58c948 2023-05-15T15:09:44+02:00 Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. Alexandra Zakharova Amanda T S Albanaz Fred R Opperdoes Ingrid Škodová-Sveráková Diana Zagirova Andreu Saura Lˇubomíra Chmelová Evgeny S Gerasimov Tereza Leštinová Tomáš Bečvář Jovana Sádlová Petr Volf Julius Lukeš Anton Horváth Anzhelika Butenko Vyacheslav Yurchenko 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010510 https://doaj.org/article/556095a013fd40e1b551ce787e58c948 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010510 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010510 https://doaj.org/article/556095a013fd40e1b551ce787e58c948 PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010510 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010510 2022-12-31T02:23:58Z Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the endosymbiotic Leishmania RNA virus 1 (LRV1) cause more severe form of the disease in a mouse model than those that do not. The presence of the virus was implicated into the parasite's replication and spreading. In this respect, studying the molecular mechanisms of cellular control of viral infection is of great medical importance. Here, we report ~30.5 Mb high-quality genome assembly of the LRV1-positive L. guyanensis M4147. This strain was turned into a model by establishing the CRISPR-Cas9 system and ablating the gene encoding phosphatidate phosphatase 2-like (PAP2L) protein. The orthologue of this gene is conspicuously absent from the genome of an unusual member of the family Trypanosomatidae, Vickermania ingenoplastis, a species with mostly bi-flagellated cells. Our analysis of the PAP2L-null L. guyanensis showed an increase in the number of cells strikingly resembling the bi-flagellated V. ingenoplastis, likely as a result of the disruption of the cell cycle, significant accumulation of phosphatidic acid, and increased virulence compared to the wild type cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 6 e0010510 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alexandra Zakharova Amanda T S Albanaz Fred R Opperdoes Ingrid Škodová-Sveráková Diana Zagirova Andreu Saura Lˇubomíra Chmelová Evgeny S Gerasimov Tereza Leštinová Tomáš Bečvář Jovana Sádlová Petr Volf Julius Lukeš Anton Horváth Anzhelika Butenko Vyacheslav Yurchenko Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leishmaniasis is a parasitic vector-borne disease caused by the protistan flagellates of the genus Leishmania. Leishmania (Viannia) guyanensis is one of the most common causative agents of the American tegumentary leishmaniasis. It has previously been shown that L. guyanensis strains that carry the endosymbiotic Leishmania RNA virus 1 (LRV1) cause more severe form of the disease in a mouse model than those that do not. The presence of the virus was implicated into the parasite's replication and spreading. In this respect, studying the molecular mechanisms of cellular control of viral infection is of great medical importance. Here, we report ~30.5 Mb high-quality genome assembly of the LRV1-positive L. guyanensis M4147. This strain was turned into a model by establishing the CRISPR-Cas9 system and ablating the gene encoding phosphatidate phosphatase 2-like (PAP2L) protein. The orthologue of this gene is conspicuously absent from the genome of an unusual member of the family Trypanosomatidae, Vickermania ingenoplastis, a species with mostly bi-flagellated cells. Our analysis of the PAP2L-null L. guyanensis showed an increase in the number of cells strikingly resembling the bi-flagellated V. ingenoplastis, likely as a result of the disruption of the cell cycle, significant accumulation of phosphatidic acid, and increased virulence compared to the wild type cells. |
format |
Article in Journal/Newspaper |
author |
Alexandra Zakharova Amanda T S Albanaz Fred R Opperdoes Ingrid Škodová-Sveráková Diana Zagirova Andreu Saura Lˇubomíra Chmelová Evgeny S Gerasimov Tereza Leštinová Tomáš Bečvář Jovana Sádlová Petr Volf Julius Lukeš Anton Horváth Anzhelika Butenko Vyacheslav Yurchenko |
author_facet |
Alexandra Zakharova Amanda T S Albanaz Fred R Opperdoes Ingrid Škodová-Sveráková Diana Zagirova Andreu Saura Lˇubomíra Chmelová Evgeny S Gerasimov Tereza Leštinová Tomáš Bečvář Jovana Sádlová Petr Volf Julius Lukeš Anton Horváth Anzhelika Butenko Vyacheslav Yurchenko |
author_sort |
Alexandra Zakharova |
title |
Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
title_short |
Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
title_full |
Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
title_fullStr |
Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
title_full_unstemmed |
Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
title_sort |
leishmania guyanensis m4147 as a new lrv1-bearing model parasite: phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010510 https://doaj.org/article/556095a013fd40e1b551ce787e58c948 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010510 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010510 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010510 https://doaj.org/article/556095a013fd40e1b551ce787e58c948 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010510 |
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PLOS Neglected Tropical Diseases |
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16 |
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6 |
container_start_page |
e0010510 |
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