Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity
Abstract Many psychrophilic microorganisms synthesize ice-binding proteins (IBPs) to survive the cold. The functions of IBPs are evaluated by the effect of the proteins on the nonequilibrium water freezing-point depression, which is called “thermal hysteresis (TH)”, and the inhibitory effect of the...
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ftdoajarticles:oai:doaj.org/article:5552710c34c6481aad72fb0792671f7e 2023-05-15T15:07:38+02:00 Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity Tatsuya Arai Akari Yamauchi Yue Yang Shiv Mohan Singh Yuji C. Sasaki Sakae Tsuda 2022-09-01T00:00:00Z https://doi.org/10.1038/s41598-022-19803-3 https://doaj.org/article/5552710c34c6481aad72fb0792671f7e EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-19803-3 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-19803-3 2045-2322 https://doaj.org/article/5552710c34c6481aad72fb0792671f7e Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-19803-3 2022-12-30T20:38:34Z Abstract Many psychrophilic microorganisms synthesize ice-binding proteins (IBPs) to survive the cold. The functions of IBPs are evaluated by the effect of the proteins on the nonequilibrium water freezing-point depression, which is called “thermal hysteresis (TH)”, and the inhibitory effect of the proteins on the growth of larger ice crystals, which is called “ice recrystallization inhibition (IRI)”. To obtain mechanical insight into the two activities, we developed a modified method of ice affinity purification and extracted two new IBP isoforms from Psychromyces glacialis, an Arctic glacier fungus. One isoform was found to be an approximately 25 kDa protein (PsgIBP_S), while the other is a 28 kDa larger protein (PsgIBP_L) that forms an intermolecular dimer. Their TH activities were less than 1 °C at millimolar concentrations, implying that both isoforms are moderately active but not hyperactive IBP species. It further appeared that both isoforms exhibit high IRI activity even at submicromolar concentrations. Furthermore, the isoforms can bind to the whole surface of a hemispherical single ice crystal, although such ice-binding was generally observed for hyperactive IBP species. These results suggest that the binding ability of IBPs to whole ice crystal surfaces is deficient for hyperactivity but is crucial for significant IRI activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Scientific Reports 12 1 |
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Directory of Open Access Journals: DOAJ Articles |
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Medicine R Science Q |
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Medicine R Science Q Tatsuya Arai Akari Yamauchi Yue Yang Shiv Mohan Singh Yuji C. Sasaki Sakae Tsuda Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
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Medicine R Science Q |
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Abstract Many psychrophilic microorganisms synthesize ice-binding proteins (IBPs) to survive the cold. The functions of IBPs are evaluated by the effect of the proteins on the nonequilibrium water freezing-point depression, which is called “thermal hysteresis (TH)”, and the inhibitory effect of the proteins on the growth of larger ice crystals, which is called “ice recrystallization inhibition (IRI)”. To obtain mechanical insight into the two activities, we developed a modified method of ice affinity purification and extracted two new IBP isoforms from Psychromyces glacialis, an Arctic glacier fungus. One isoform was found to be an approximately 25 kDa protein (PsgIBP_S), while the other is a 28 kDa larger protein (PsgIBP_L) that forms an intermolecular dimer. Their TH activities were less than 1 °C at millimolar concentrations, implying that both isoforms are moderately active but not hyperactive IBP species. It further appeared that both isoforms exhibit high IRI activity even at submicromolar concentrations. Furthermore, the isoforms can bind to the whole surface of a hemispherical single ice crystal, although such ice-binding was generally observed for hyperactive IBP species. These results suggest that the binding ability of IBPs to whole ice crystal surfaces is deficient for hyperactivity but is crucial for significant IRI activity. |
format |
Article in Journal/Newspaper |
author |
Tatsuya Arai Akari Yamauchi Yue Yang Shiv Mohan Singh Yuji C. Sasaki Sakae Tsuda |
author_facet |
Tatsuya Arai Akari Yamauchi Yue Yang Shiv Mohan Singh Yuji C. Sasaki Sakae Tsuda |
author_sort |
Tatsuya Arai |
title |
Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
title_short |
Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
title_full |
Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
title_fullStr |
Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
title_full_unstemmed |
Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
title_sort |
adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-19803-3 https://doaj.org/article/5552710c34c6481aad72fb0792671f7e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-19803-3 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-19803-3 2045-2322 https://doaj.org/article/5552710c34c6481aad72fb0792671f7e |
op_doi |
https://doi.org/10.1038/s41598-022-19803-3 |
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Scientific Reports |
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12 |
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1 |
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1766339099993571328 |