Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay.
Visceral leishmaniasis (VL) is a vector-borne protozoan disease, which can be fatal if left untreated. Synthetic chemical insecticides are very effective tools for controlling of insect vectors, including the sand fly Phlebotomus argentipes, the vector of VL in the Indian subcontinent. However, repe...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2023
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0011276 https://doaj.org/article/55483d20a4314576982e1b41aeee9740 |
id |
ftdoajarticles:oai:doaj.org/article:55483d20a4314576982e1b41aeee9740 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:55483d20a4314576982e1b41aeee9740 2023-07-02T03:31:35+02:00 Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. Rahul Chaubey Ashish Shukla Anurag Kumar Kushwaha Puja Tiwary Shakti Kumar Singh Shawna Hennings Om Praksh Singh Phillip Lawyer Edgar Rowton Christine A Petersen Scott A Bernhardt Shyam Sundar 2023-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011276 https://doaj.org/article/55483d20a4314576982e1b41aeee9740 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011276 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011276 https://doaj.org/article/55483d20a4314576982e1b41aeee9740 PLoS Neglected Tropical Diseases, Vol 17, Iss 5, p e0011276 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011276 2023-06-11T00:37:56Z Visceral leishmaniasis (VL) is a vector-borne protozoan disease, which can be fatal if left untreated. Synthetic chemical insecticides are very effective tools for controlling of insect vectors, including the sand fly Phlebotomus argentipes, the vector of VL in the Indian subcontinent. However, repeated use of the same insecticide with increasing doses potentially can create high selection pressure and lead to tolerance and resistance development. The objective of this study was to determine the lethal concentrations and assess levels of susceptibility, diagnostic doses and times to death of laboratory-reared P. argentipes to five insecticides that are used worldwide to control vectors. Using the Center for Disease Control and Prevention (CDC) bottle bioassay, 20-30 sand flies were exposed in insecticide- coated 500-ml glass bottles. Flies were then observed for 24 hours and mortality was recorded. Dose-response survival curves were generated for each insecticide using QCal software and lethal concentrations causing 50%, 90% and 95% mortality were determined. A bioassay was also conducted to determine diagnostic doses and diagnostic times by exposing 20-30 flies in each bottle containing set concentrations of insecticide. Mortality was recorded at 10-minute intervals for 120 minutes to generate the survival curve. Phlebotomus argentipes are highly susceptible to alpha-cypermethrin, followed by deltamethrin, malathion, chlorpyrifos, and least susceptible to DDT. Also, the lowest diagnostic doses and diagnostic times were established for alpha-cypermethrin (3μg/ml for 40 minutes) to kill 100% of the flies. The susceptibility data, diagnostic doses and diagnostic times presented here will be useful as baseline reference points for future studies to assess insecticide susceptibility and resistance monitoring of field caught sand flies and to assist in surveillance as VL elimination is achieved in the region. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian PLOS Neglected Tropical Diseases 17 5 e0011276 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rahul Chaubey Ashish Shukla Anurag Kumar Kushwaha Puja Tiwary Shakti Kumar Singh Shawna Hennings Om Praksh Singh Phillip Lawyer Edgar Rowton Christine A Petersen Scott A Bernhardt Shyam Sundar Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Visceral leishmaniasis (VL) is a vector-borne protozoan disease, which can be fatal if left untreated. Synthetic chemical insecticides are very effective tools for controlling of insect vectors, including the sand fly Phlebotomus argentipes, the vector of VL in the Indian subcontinent. However, repeated use of the same insecticide with increasing doses potentially can create high selection pressure and lead to tolerance and resistance development. The objective of this study was to determine the lethal concentrations and assess levels of susceptibility, diagnostic doses and times to death of laboratory-reared P. argentipes to five insecticides that are used worldwide to control vectors. Using the Center for Disease Control and Prevention (CDC) bottle bioassay, 20-30 sand flies were exposed in insecticide- coated 500-ml glass bottles. Flies were then observed for 24 hours and mortality was recorded. Dose-response survival curves were generated for each insecticide using QCal software and lethal concentrations causing 50%, 90% and 95% mortality were determined. A bioassay was also conducted to determine diagnostic doses and diagnostic times by exposing 20-30 flies in each bottle containing set concentrations of insecticide. Mortality was recorded at 10-minute intervals for 120 minutes to generate the survival curve. Phlebotomus argentipes are highly susceptible to alpha-cypermethrin, followed by deltamethrin, malathion, chlorpyrifos, and least susceptible to DDT. Also, the lowest diagnostic doses and diagnostic times were established for alpha-cypermethrin (3μg/ml for 40 minutes) to kill 100% of the flies. The susceptibility data, diagnostic doses and diagnostic times presented here will be useful as baseline reference points for future studies to assess insecticide susceptibility and resistance monitoring of field caught sand flies and to assist in surveillance as VL elimination is achieved in the region. |
format |
Article in Journal/Newspaper |
author |
Rahul Chaubey Ashish Shukla Anurag Kumar Kushwaha Puja Tiwary Shakti Kumar Singh Shawna Hennings Om Praksh Singh Phillip Lawyer Edgar Rowton Christine A Petersen Scott A Bernhardt Shyam Sundar |
author_facet |
Rahul Chaubey Ashish Shukla Anurag Kumar Kushwaha Puja Tiwary Shakti Kumar Singh Shawna Hennings Om Praksh Singh Phillip Lawyer Edgar Rowton Christine A Petersen Scott A Bernhardt Shyam Sundar |
author_sort |
Rahul Chaubey |
title |
Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
title_short |
Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
title_full |
Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
title_fullStr |
Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
title_full_unstemmed |
Assessing insecticide susceptibility, diagnostic dose and time for the sand fly Phlebotomus argentipes, the vector of visceral leishmaniasis in India, using the CDC bottle bioassay. |
title_sort |
assessing insecticide susceptibility, diagnostic dose and time for the sand fly phlebotomus argentipes, the vector of visceral leishmaniasis in india, using the cdc bottle bioassay. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011276 https://doaj.org/article/55483d20a4314576982e1b41aeee9740 |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 5, p e0011276 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011276 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011276 https://doaj.org/article/55483d20a4314576982e1b41aeee9740 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011276 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
5 |
container_start_page |
e0011276 |
_version_ |
1770270950856589312 |