EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS
In Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a pu...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:54fb4b95ea3548feb781adcc7fa18b21 2024-09-09T19:26:59+00:00 EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS José Geraldo Castro-Júnior Mariana Lourenço Freire Samantha Priscila Silva Campos Kezia K.G. Scopel Renato Porrozzi Edimilson Domingos Da Silva Fabio A. Colombo Rita de Cássia Viveiros da Silveira Marcos José Marques Elaine Soares Coimbra 2014-06-01T00:00:00Z https://doi.org/10.1590/S0036-46652014000300008 https://doaj.org/article/54fb4b95ea3548feb781adcc7fa18b21 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000300225&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000300008 https://doaj.org/article/54fb4b95ea3548feb781adcc7fa18b21 Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 3, Pp 225-229 (2014) Canine visceral leishmaniasis Serological diagnosis Immunochromatographic diagnosis DPP PCR diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1590/S0036-46652014000300008 2024-08-05T17:49:30Z In Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a public kennel in the municipality of Juiz de Fora, Minas Gerais State, Brazil, using the immunochromatographic TR DPP® CVL rapid test. Additionally, conventional and/or real time PCR assay was used to detect and confirm L. infantum infection in the DPP positive dogs only. Of the 400 dogs studied, most did not present clinical signs for CVL (p < 0.05), and fifteen (3.8%) were seropositive in the DPP test. There was no statistically significant difference between the DPP seropositive dogs and the clinical signs of the disease (p > 0.05). Both conventional and real time PCR tests confirmed L. infantum infection in nine (75.0%) of the twelve DPP seropositive dogs that remained alive during the follow-up period. This study is the first seroepidemiologic survey of CVL held in the city of Juiz de Fora, and the results reinforce the idea that this disease is currently in a process of expansion and urbanization in Brazil. Furthermore, this study highlights the use of the DPP test as an alternative for diagnosing CVL in large and mid-sized cities, due to its ease of implementation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 56 3 225 229 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Canine visceral leishmaniasis Serological diagnosis Immunochromatographic diagnosis DPP PCR diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Canine visceral leishmaniasis Serological diagnosis Immunochromatographic diagnosis DPP PCR diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 José Geraldo Castro-Júnior Mariana Lourenço Freire Samantha Priscila Silva Campos Kezia K.G. Scopel Renato Porrozzi Edimilson Domingos Da Silva Fabio A. Colombo Rita de Cássia Viveiros da Silveira Marcos José Marques Elaine Soares Coimbra EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
topic_facet |
Canine visceral leishmaniasis Serological diagnosis Immunochromatographic diagnosis DPP PCR diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a public kennel in the municipality of Juiz de Fora, Minas Gerais State, Brazil, using the immunochromatographic TR DPP® CVL rapid test. Additionally, conventional and/or real time PCR assay was used to detect and confirm L. infantum infection in the DPP positive dogs only. Of the 400 dogs studied, most did not present clinical signs for CVL (p < 0.05), and fifteen (3.8%) were seropositive in the DPP test. There was no statistically significant difference between the DPP seropositive dogs and the clinical signs of the disease (p > 0.05). Both conventional and real time PCR tests confirmed L. infantum infection in nine (75.0%) of the twelve DPP seropositive dogs that remained alive during the follow-up period. This study is the first seroepidemiologic survey of CVL held in the city of Juiz de Fora, and the results reinforce the idea that this disease is currently in a process of expansion and urbanization in Brazil. Furthermore, this study highlights the use of the DPP test as an alternative for diagnosing CVL in large and mid-sized cities, due to its ease of implementation. |
format |
Article in Journal/Newspaper |
author |
José Geraldo Castro-Júnior Mariana Lourenço Freire Samantha Priscila Silva Campos Kezia K.G. Scopel Renato Porrozzi Edimilson Domingos Da Silva Fabio A. Colombo Rita de Cássia Viveiros da Silveira Marcos José Marques Elaine Soares Coimbra |
author_facet |
José Geraldo Castro-Júnior Mariana Lourenço Freire Samantha Priscila Silva Campos Kezia K.G. Scopel Renato Porrozzi Edimilson Domingos Da Silva Fabio A. Colombo Rita de Cássia Viveiros da Silveira Marcos José Marques Elaine Soares Coimbra |
author_sort |
José Geraldo Castro-Júnior |
title |
EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
title_short |
EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
title_full |
EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
title_fullStr |
EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
title_full_unstemmed |
EVIDENCE OF Leishmania (Leishmania) infantum INFECTION IN DOGS FROM JUIZ DE FORA, MINAS GERAIS STATE, BRAZIL, BASED ON IMMUNOCHROMATOGRAPHIC DUAL-PATH PLATFORM (DPP®) AND PCR ASSAYS |
title_sort |
evidence of leishmania (leishmania) infantum infection in dogs from juiz de fora, minas gerais state, brazil, based on immunochromatographic dual-path platform (dpp®) and pcr assays |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2014 |
url |
https://doi.org/10.1590/S0036-46652014000300008 https://doaj.org/article/54fb4b95ea3548feb781adcc7fa18b21 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 3, Pp 225-229 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000300225&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000300008 https://doaj.org/article/54fb4b95ea3548feb781adcc7fa18b21 |
op_doi |
https://doi.org/10.1590/S0036-46652014000300008 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
56 |
container_issue |
3 |
container_start_page |
225 |
op_container_end_page |
229 |
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