Mucus: aiding elasmobranch conservation through non-invasive genetic sampling
Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and implemented mucus swabbing for a fr...
Published in: | Endangered Species Research |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Inter-Research
2013
|
Subjects: | |
Online Access: | https://doi.org/10.3354/esr00524 https://doaj.org/article/54d30ac65d5f4db8b1afe8245e99b40c |
id |
ftdoajarticles:oai:doaj.org/article:54d30ac65d5f4db8b1afe8245e99b40c |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:54d30ac65d5f4db8b1afe8245e99b40c 2023-05-15T15:53:51+02:00 Mucus: aiding elasmobranch conservation through non-invasive genetic sampling L Lieber S Berrow E Johnston G Hall J Hall C Gubili DW Sims CS Jones LR Noble 2013-09-01T00:00:00Z https://doi.org/10.3354/esr00524 https://doaj.org/article/54d30ac65d5f4db8b1afe8245e99b40c EN eng Inter-Research https://www.int-res.com/abstracts/esr/v21/n3/p215-222/ https://doaj.org/toc/1863-5407 https://doaj.org/toc/1613-4796 1863-5407 1613-4796 doi:10.3354/esr00524 https://doaj.org/article/54d30ac65d5f4db8b1afe8245e99b40c Endangered Species Research, Vol 21, Iss 3, Pp 215-222 (2013) Zoology QL1-991 Botany QK1-989 article 2013 ftdoajarticles https://doi.org/10.3354/esr00524 2022-12-30T20:23:08Z Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and implemented mucus swabbing for a free-ranging elasmobranch, thereby demonstrating the utility of this method in the field. We report the first attempt at mucus collection from 30 plankton-feeding basking sharks Cetorhinus maximus from 3 spatially distinct ‘hotspots’ in Irish waters. C. maximus DNA was successfully extracted and verified using DNA barcoding of the mitochondrial DNA cytochrome c oxidase 1 gene (99% sequence similarity) and basking shark species-specific multiplex PCRs derived from the nuclear ribosomal internal transcribed spacer 2 locus. Mitochondrial control region sequencing (1086 bp) showed that Irish samples were dominated by 2 haplotypes previously found to be globally distributed. Additionally, 1 novel haplotype was defined from western County Kerry. On-going genetic tagging will eventually provide more accurate estimates of global basking shark population structuring, abundance and behavioural ecology. Article in Journal/Newspaper Cetorhinus maximus Directory of Open Access Journals: DOAJ Articles Endangered Species Research 21 3 215 222 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Zoology QL1-991 Botany QK1-989 |
spellingShingle |
Zoology QL1-991 Botany QK1-989 L Lieber S Berrow E Johnston G Hall J Hall C Gubili DW Sims CS Jones LR Noble Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
topic_facet |
Zoology QL1-991 Botany QK1-989 |
description |
Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and implemented mucus swabbing for a free-ranging elasmobranch, thereby demonstrating the utility of this method in the field. We report the first attempt at mucus collection from 30 plankton-feeding basking sharks Cetorhinus maximus from 3 spatially distinct ‘hotspots’ in Irish waters. C. maximus DNA was successfully extracted and verified using DNA barcoding of the mitochondrial DNA cytochrome c oxidase 1 gene (99% sequence similarity) and basking shark species-specific multiplex PCRs derived from the nuclear ribosomal internal transcribed spacer 2 locus. Mitochondrial control region sequencing (1086 bp) showed that Irish samples were dominated by 2 haplotypes previously found to be globally distributed. Additionally, 1 novel haplotype was defined from western County Kerry. On-going genetic tagging will eventually provide more accurate estimates of global basking shark population structuring, abundance and behavioural ecology. |
format |
Article in Journal/Newspaper |
author |
L Lieber S Berrow E Johnston G Hall J Hall C Gubili DW Sims CS Jones LR Noble |
author_facet |
L Lieber S Berrow E Johnston G Hall J Hall C Gubili DW Sims CS Jones LR Noble |
author_sort |
L Lieber |
title |
Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
title_short |
Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
title_full |
Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
title_fullStr |
Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
title_full_unstemmed |
Mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
title_sort |
mucus: aiding elasmobranch conservation through non-invasive genetic sampling |
publisher |
Inter-Research |
publishDate |
2013 |
url |
https://doi.org/10.3354/esr00524 https://doaj.org/article/54d30ac65d5f4db8b1afe8245e99b40c |
genre |
Cetorhinus maximus |
genre_facet |
Cetorhinus maximus |
op_source |
Endangered Species Research, Vol 21, Iss 3, Pp 215-222 (2013) |
op_relation |
https://www.int-res.com/abstracts/esr/v21/n3/p215-222/ https://doaj.org/toc/1863-5407 https://doaj.org/toc/1613-4796 1863-5407 1613-4796 doi:10.3354/esr00524 https://doaj.org/article/54d30ac65d5f4db8b1afe8245e99b40c |
op_doi |
https://doi.org/10.3354/esr00524 |
container_title |
Endangered Species Research |
container_volume |
21 |
container_issue |
3 |
container_start_page |
215 |
op_container_end_page |
222 |
_version_ |
1766389024543473664 |