Identification of Plasmodium falciparum proteoforms from liver stage models
Abstract Background Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including...
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ftdoajarticles:oai:doaj.org/article:54c91dfe7578497593d4de236d8ff4f4 2023-05-15T15:05:17+02:00 Identification of Plasmodium falciparum proteoforms from liver stage models Benjamin Winer Kimberly A. Edgel Xiaoyan Zou Julie Sellau Sri Hadiwidjojo Lindsey S. Garver Christin E. McDonough Neil L. Kelleher Paul M. Thomas Eileen Villasante Alexander Ploss Vincent R. Gerbasi 2020-01-01T00:00:00Z https://doi.org/10.1186/s12936-019-3093-3 https://doaj.org/article/54c91dfe7578497593d4de236d8ff4f4 EN eng BMC https://doi.org/10.1186/s12936-019-3093-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3093-3 1475-2875 https://doaj.org/article/54c91dfe7578497593d4de236d8ff4f4 Malaria Journal, Vol 19, Iss 1, Pp 1-13 (2020) Proteomics Liver stage Top-down Vaccine Antigen Cell-mediated immunity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-019-3093-3 2022-12-31T12:08:25Z Abstract Background Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known. Methods In this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state. Results These results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold. Conclusions This work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Proteomics Liver stage Top-down Vaccine Antigen Cell-mediated immunity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Proteomics Liver stage Top-down Vaccine Antigen Cell-mediated immunity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Benjamin Winer Kimberly A. Edgel Xiaoyan Zou Julie Sellau Sri Hadiwidjojo Lindsey S. Garver Christin E. McDonough Neil L. Kelleher Paul M. Thomas Eileen Villasante Alexander Ploss Vincent R. Gerbasi Identification of Plasmodium falciparum proteoforms from liver stage models |
topic_facet |
Proteomics Liver stage Top-down Vaccine Antigen Cell-mediated immunity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known. Methods In this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state. Results These results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold. Conclusions This work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development. |
format |
Article in Journal/Newspaper |
author |
Benjamin Winer Kimberly A. Edgel Xiaoyan Zou Julie Sellau Sri Hadiwidjojo Lindsey S. Garver Christin E. McDonough Neil L. Kelleher Paul M. Thomas Eileen Villasante Alexander Ploss Vincent R. Gerbasi |
author_facet |
Benjamin Winer Kimberly A. Edgel Xiaoyan Zou Julie Sellau Sri Hadiwidjojo Lindsey S. Garver Christin E. McDonough Neil L. Kelleher Paul M. Thomas Eileen Villasante Alexander Ploss Vincent R. Gerbasi |
author_sort |
Benjamin Winer |
title |
Identification of Plasmodium falciparum proteoforms from liver stage models |
title_short |
Identification of Plasmodium falciparum proteoforms from liver stage models |
title_full |
Identification of Plasmodium falciparum proteoforms from liver stage models |
title_fullStr |
Identification of Plasmodium falciparum proteoforms from liver stage models |
title_full_unstemmed |
Identification of Plasmodium falciparum proteoforms from liver stage models |
title_sort |
identification of plasmodium falciparum proteoforms from liver stage models |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-019-3093-3 https://doaj.org/article/54c91dfe7578497593d4de236d8ff4f4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-13 (2020) |
op_relation |
https://doi.org/10.1186/s12936-019-3093-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3093-3 1475-2875 https://doaj.org/article/54c91dfe7578497593d4de236d8ff4f4 |
op_doi |
https://doi.org/10.1186/s12936-019-3093-3 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766337018980204544 |