A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells.
Little is known of the direct microbicidal activity of T cells in leprosy, so a lipopeptide consisting of the N-terminal 13 amino acids lipopeptide (LipoK) of a 33-kD lipoprotein of Mycobacterium leprae, was synthesized. LipoK activated M. leprae infected human dendritic cells (DCs) to induce the pr...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0001401 https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d |
id |
ftdoajarticles:oai:doaj.org/article:54b80051cdb74e6db95a50dfcc07424d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:54b80051cdb74e6db95a50dfcc07424d 2023-05-15T15:06:56+02:00 A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. Yumi Maeda Toshiki Tamura Yasuo Fukutomi Tetsu Mukai Masanori Kai Masahiko Makino 2011-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001401 https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3222628?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001401 https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1401 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001401 2022-12-30T21:08:54Z Little is known of the direct microbicidal activity of T cells in leprosy, so a lipopeptide consisting of the N-terminal 13 amino acids lipopeptide (LipoK) of a 33-kD lipoprotein of Mycobacterium leprae, was synthesized. LipoK activated M. leprae infected human dendritic cells (DCs) to induce the production of IL-12. These activated DCs stimulated autologous CD4+ or CD8+ T cells towards type 1 immune response by inducing interferon-gamma secretion. T cell proliferation was also evident from the CFSE labeling of target CD4+ or CD8+ T cells. The direct microbicidal activity of T cells in the control of M. leprae multiplication is not well understood. The present study showed significant production of granulysin, granzyme B and perforin from these activated CD4+ and CD8+ T cells when stimulated with LipoK activated, M. leprae infected DCs. Assessment of the viability of M. leprae in DCs indicated LipoK mediated T cell-dependent killing of M. leprae. Remarkably, granulysin as well as granzyme B could directly kill M. leprae in vitro. Our results provide evidence that LipoK could facilitate M. leprae killing through the production of effector molecules granulysin and granzyme B in T cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 11 e1401 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yumi Maeda Toshiki Tamura Yasuo Fukutomi Tetsu Mukai Masanori Kai Masahiko Makino A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Little is known of the direct microbicidal activity of T cells in leprosy, so a lipopeptide consisting of the N-terminal 13 amino acids lipopeptide (LipoK) of a 33-kD lipoprotein of Mycobacterium leprae, was synthesized. LipoK activated M. leprae infected human dendritic cells (DCs) to induce the production of IL-12. These activated DCs stimulated autologous CD4+ or CD8+ T cells towards type 1 immune response by inducing interferon-gamma secretion. T cell proliferation was also evident from the CFSE labeling of target CD4+ or CD8+ T cells. The direct microbicidal activity of T cells in the control of M. leprae multiplication is not well understood. The present study showed significant production of granulysin, granzyme B and perforin from these activated CD4+ and CD8+ T cells when stimulated with LipoK activated, M. leprae infected DCs. Assessment of the viability of M. leprae in DCs indicated LipoK mediated T cell-dependent killing of M. leprae. Remarkably, granulysin as well as granzyme B could directly kill M. leprae in vitro. Our results provide evidence that LipoK could facilitate M. leprae killing through the production of effector molecules granulysin and granzyme B in T cells. |
format |
Article in Journal/Newspaper |
author |
Yumi Maeda Toshiki Tamura Yasuo Fukutomi Tetsu Mukai Masanori Kai Masahiko Makino |
author_facet |
Yumi Maeda Toshiki Tamura Yasuo Fukutomi Tetsu Mukai Masanori Kai Masahiko Makino |
author_sort |
Yumi Maeda |
title |
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
title_short |
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
title_full |
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
title_fullStr |
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
title_full_unstemmed |
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
title_sort |
lipopeptide facilitate induction of mycobacterium leprae killing in host cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001401 https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1401 (2011) |
op_relation |
http://europepmc.org/articles/PMC3222628?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001401 https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d |
op_doi |
https://doi.org/10.1371/journal.pntd.0001401 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
11 |
container_start_page |
e1401 |
_version_ |
1766338525403283456 |