Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART)
The aim of this study was to evaluate the genotypic resistance profiles of HIV-1 in children failing highly active antiretroviral therapy (HAART). Forty-one children (median age = 67 months) receiving HAART were submitted to genotypic testing when virological failure was detected. cDNA was extracted...
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ftdoajarticles:oai:doaj.org/article:54b2c57a51e14942beb01886968c786c 2024-09-09T19:28:30+00:00 Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) Daisy Maria Machado Silvana Cláudia Fernandes Regina Célia de Menezes Succi Wilton Santos Freire Cláudio Sérgio Pannuti Aída Barbosa Gouveia José Eduardo Levi Ricardo Sobie Diaz 2005-02-01T00:00:00Z https://doi.org/10.1590/S0036-46652005000100001 https://doaj.org/article/54b2c57a51e14942beb01886968c786c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000100001 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000100001 0036-4665 1678-9946 https://doaj.org/article/54b2c57a51e14942beb01886968c786c Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 1, Pp 1-5 (2005) HIV resistance Antiretroviral therapy Children Treatment failure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2005 ftdoajarticles https://doi.org/10.1590/S0036-46652005000100001 2024-08-05T17:49:30Z The aim of this study was to evaluate the genotypic resistance profiles of HIV-1 in children failing highly active antiretroviral therapy (HAART). Forty-one children (median age = 67 months) receiving HAART were submitted to genotypic testing when virological failure was detected. cDNA was extracted from PBMCs and amplified by nested PCR for the reverse transcriptase and protease regions of the pol gene. Drug resistance genotypes were determined from DNA sequencing. According to the genotypic analysis, 12/36 (33.3%) and 6/36 (16.6%) children showed resistance and possible resistance, respectively, to ZDV; 5/36 (14%) and 4/36 (11.1%), respectively, showed resistance and possible resistance to ddI; 4/36 (11.1%) showed resistance to 3TC and D4T; and 3/36 (8.3%) showed resistance to Abacavir. A high percentage (54%) of children exhibited mutations conferring resistance to NNRTI class drugs. Respective rates of resistance and possible resistance to PIs were: RTV (12.2%, 7.3%); APV (2.4%, 12.1%); SQV(0%, 12.1%); IDV (14.6%, 4.9%), NFV (22%, 4.9%), LPV/RTV (2.4%, 12.1%). Overall, 37/41 (90%) children exhibited virus with mutations related to drug resistance, while 9% exhibited resistance to all three antiretroviral drug classes. O objetivo deste estudo foi avaliar o perfil de resistência genotípica do HIV-1 em crianças com falha terapêutica ao tratamento anti-retroviral (HAART). Quarenta e uma crianças (idade mediana = 67 meses) em uso de HAART foram submetidas ao teste de genotipagem no momento da detecção de falha ao tratamento. Foi realizada extração de cDNA de células periféricas mononucleares e amplificação do mesmo (regiões da transcriptase reversa e protease do gene pol) através de PCR-nested. O perfil genotípico foi determinado através do seqüenciamnto de nucleotídeos. De acordo com a análise genotípica, 12/36 (33,3%) e 6/36 (16,6%) crianças apresentaram, respectivamente, resistência e possível resistência ao AZT; 5/36 (14%) e 4/36 (11,1%), respectivamente, eram resistentes e possivelmente resistentes ao ddI; ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 47 1 1 5 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
HIV resistance Antiretroviral therapy Children Treatment failure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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HIV resistance Antiretroviral therapy Children Treatment failure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Daisy Maria Machado Silvana Cláudia Fernandes Regina Célia de Menezes Succi Wilton Santos Freire Cláudio Sérgio Pannuti Aída Barbosa Gouveia José Eduardo Levi Ricardo Sobie Diaz Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
topic_facet |
HIV resistance Antiretroviral therapy Children Treatment failure Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The aim of this study was to evaluate the genotypic resistance profiles of HIV-1 in children failing highly active antiretroviral therapy (HAART). Forty-one children (median age = 67 months) receiving HAART were submitted to genotypic testing when virological failure was detected. cDNA was extracted from PBMCs and amplified by nested PCR for the reverse transcriptase and protease regions of the pol gene. Drug resistance genotypes were determined from DNA sequencing. According to the genotypic analysis, 12/36 (33.3%) and 6/36 (16.6%) children showed resistance and possible resistance, respectively, to ZDV; 5/36 (14%) and 4/36 (11.1%), respectively, showed resistance and possible resistance to ddI; 4/36 (11.1%) showed resistance to 3TC and D4T; and 3/36 (8.3%) showed resistance to Abacavir. A high percentage (54%) of children exhibited mutations conferring resistance to NNRTI class drugs. Respective rates of resistance and possible resistance to PIs were: RTV (12.2%, 7.3%); APV (2.4%, 12.1%); SQV(0%, 12.1%); IDV (14.6%, 4.9%), NFV (22%, 4.9%), LPV/RTV (2.4%, 12.1%). Overall, 37/41 (90%) children exhibited virus with mutations related to drug resistance, while 9% exhibited resistance to all three antiretroviral drug classes. O objetivo deste estudo foi avaliar o perfil de resistência genotípica do HIV-1 em crianças com falha terapêutica ao tratamento anti-retroviral (HAART). Quarenta e uma crianças (idade mediana = 67 meses) em uso de HAART foram submetidas ao teste de genotipagem no momento da detecção de falha ao tratamento. Foi realizada extração de cDNA de células periféricas mononucleares e amplificação do mesmo (regiões da transcriptase reversa e protease do gene pol) através de PCR-nested. O perfil genotípico foi determinado através do seqüenciamnto de nucleotídeos. De acordo com a análise genotípica, 12/36 (33,3%) e 6/36 (16,6%) crianças apresentaram, respectivamente, resistência e possível resistência ao AZT; 5/36 (14%) e 4/36 (11,1%), respectivamente, eram resistentes e possivelmente resistentes ao ddI; ... |
format |
Article in Journal/Newspaper |
author |
Daisy Maria Machado Silvana Cláudia Fernandes Regina Célia de Menezes Succi Wilton Santos Freire Cláudio Sérgio Pannuti Aída Barbosa Gouveia José Eduardo Levi Ricardo Sobie Diaz |
author_facet |
Daisy Maria Machado Silvana Cláudia Fernandes Regina Célia de Menezes Succi Wilton Santos Freire Cláudio Sérgio Pannuti Aída Barbosa Gouveia José Eduardo Levi Ricardo Sobie Diaz |
author_sort |
Daisy Maria Machado |
title |
Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
title_short |
Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
title_full |
Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
title_fullStr |
Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
title_full_unstemmed |
Analysis of HIV- type 1 protease and reverse transcriptase in Brazilian children failing highly active antiretroviral therapy (HAART) Análise da protease e transcriptase reversa do HIV-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (HAART) |
title_sort |
analysis of hiv- type 1 protease and reverse transcriptase in brazilian children failing highly active antiretroviral therapy (haart) análise da protease e transcriptase reversa do hiv-1 em crianças com falha terapêutica em uso de terapia anti-retroviral altamente eficaz (haart) |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2005 |
url |
https://doi.org/10.1590/S0036-46652005000100001 https://doaj.org/article/54b2c57a51e14942beb01886968c786c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 1, Pp 1-5 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000100001 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000100001 0036-4665 1678-9946 https://doaj.org/article/54b2c57a51e14942beb01886968c786c |
op_doi |
https://doi.org/10.1590/S0036-46652005000100001 |
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47 |
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