Oral rapid test: an alternative to traditional HIV screening in Chile
OBJECTIVE: To compare the sensitivity and specificity of an Oral Rapid Test (ORT) to that of the Enzyme-Linked Immunosorbent Assay (ELISA) for HIV testing in Santiago, Chile; to track the number of study participants returning for ELISA testing results; and to analyze the participants' percepti...
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ftdoajarticles:oai:doaj.org/article:54afe504519e46368a04ab4536058085 2023-05-15T15:12:56+02:00 Oral rapid test: an alternative to traditional HIV screening in Chile Lisette Paola Irarrazábal Lilian Ferrer Rosina Cianelli Loreto Lara Reiley Reed Judith Levy Carlos Pérez 2013-06-01T00:00:00Z https://doaj.org/article/54afe504519e46368a04ab4536058085 EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892013000600007&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 https://doaj.org/article/54afe504519e46368a04ab4536058085 Revista Panamericana de Salud Pública, Vol 33, Iss 6, Pp 427-432 (2013) Infecciones por VIH diagnóstico serodiagnóstico del SIDA seroprevalencia de VIH técnicas de laboratorio clínico prevención de enfermedades Chile Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles 2022-12-31T14:23:04Z OBJECTIVE: To compare the sensitivity and specificity of an Oral Rapid Test (ORT) to that of the Enzyme-Linked Immunosorbent Assay (ELISA) for HIV testing in Santiago, Chile; to track the number of study participants returning for ELISA testing results; and to analyze the participants' perceptions of the ORT compared to the ELISA. METHODS: A total of 497 people were recruited in Santiago, Chile: 153 had previously tested positive for HIV, and 344 were of unknown status. Participants were tested for HIV using both the ELISA and the ORT to examine and compare specificity and sensitivity. Qualitative data were collected from 22 participants to compare perceptions of the testing experience with ORT versus ELISA. RESULTS: The ELISA reported 184 (37%) of the 497 participants as being "positive" for HIV antibodies; the ORT showed 181 (36.4%) as being "reactive" for HIV. The ORT showed a sensitivity of 98.4% (95.7%-99.9%, 95% Confidence Interval) and specificity of 100%. The Kappa test produced K = 0.983 (P < 0.0001). Of the 344 participants whose HIV status was unknown at the start of the study, 55 failed to return for their ELISA results. Participants positively perceived ORT as having reduced both waiting time and anxiety over obtaining their test results. ORT oral swabbing appeared more practical and less invasive than drawing blood for the ELISA. CONCLUSIONS: The ORT and ELISA were statistically equal in specificity and sensitivity. ORT provides quicker results, potentially ensuring that more people receive them, and does not require handling of or exposure to potentially hazardous blood products. Trial number: ClinicalTrials.gov identifier: NCT01733927. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sida ENVELOPE(101.683,101.683,67.800,67.800) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish Portuguese |
topic |
Infecciones por VIH diagnóstico serodiagnóstico del SIDA seroprevalencia de VIH técnicas de laboratorio clínico prevención de enfermedades Chile Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Infecciones por VIH diagnóstico serodiagnóstico del SIDA seroprevalencia de VIH técnicas de laboratorio clínico prevención de enfermedades Chile Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lisette Paola Irarrazábal Lilian Ferrer Rosina Cianelli Loreto Lara Reiley Reed Judith Levy Carlos Pérez Oral rapid test: an alternative to traditional HIV screening in Chile |
topic_facet |
Infecciones por VIH diagnóstico serodiagnóstico del SIDA seroprevalencia de VIH técnicas de laboratorio clínico prevención de enfermedades Chile Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
OBJECTIVE: To compare the sensitivity and specificity of an Oral Rapid Test (ORT) to that of the Enzyme-Linked Immunosorbent Assay (ELISA) for HIV testing in Santiago, Chile; to track the number of study participants returning for ELISA testing results; and to analyze the participants' perceptions of the ORT compared to the ELISA. METHODS: A total of 497 people were recruited in Santiago, Chile: 153 had previously tested positive for HIV, and 344 were of unknown status. Participants were tested for HIV using both the ELISA and the ORT to examine and compare specificity and sensitivity. Qualitative data were collected from 22 participants to compare perceptions of the testing experience with ORT versus ELISA. RESULTS: The ELISA reported 184 (37%) of the 497 participants as being "positive" for HIV antibodies; the ORT showed 181 (36.4%) as being "reactive" for HIV. The ORT showed a sensitivity of 98.4% (95.7%-99.9%, 95% Confidence Interval) and specificity of 100%. The Kappa test produced K = 0.983 (P < 0.0001). Of the 344 participants whose HIV status was unknown at the start of the study, 55 failed to return for their ELISA results. Participants positively perceived ORT as having reduced both waiting time and anxiety over obtaining their test results. ORT oral swabbing appeared more practical and less invasive than drawing blood for the ELISA. CONCLUSIONS: The ORT and ELISA were statistically equal in specificity and sensitivity. ORT provides quicker results, potentially ensuring that more people receive them, and does not require handling of or exposure to potentially hazardous blood products. Trial number: ClinicalTrials.gov identifier: NCT01733927. |
format |
Article in Journal/Newspaper |
author |
Lisette Paola Irarrazábal Lilian Ferrer Rosina Cianelli Loreto Lara Reiley Reed Judith Levy Carlos Pérez |
author_facet |
Lisette Paola Irarrazábal Lilian Ferrer Rosina Cianelli Loreto Lara Reiley Reed Judith Levy Carlos Pérez |
author_sort |
Lisette Paola Irarrazábal |
title |
Oral rapid test: an alternative to traditional HIV screening in Chile |
title_short |
Oral rapid test: an alternative to traditional HIV screening in Chile |
title_full |
Oral rapid test: an alternative to traditional HIV screening in Chile |
title_fullStr |
Oral rapid test: an alternative to traditional HIV screening in Chile |
title_full_unstemmed |
Oral rapid test: an alternative to traditional HIV screening in Chile |
title_sort |
oral rapid test: an alternative to traditional hiv screening in chile |
publisher |
Pan American Health Organization |
publishDate |
2013 |
url |
https://doaj.org/article/54afe504519e46368a04ab4536058085 |
long_lat |
ENVELOPE(101.683,101.683,67.800,67.800) |
geographic |
Arctic Sida |
geographic_facet |
Arctic Sida |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 33, Iss 6, Pp 427-432 (2013) |
op_relation |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892013000600007&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 https://doaj.org/article/54afe504519e46368a04ab4536058085 |
_version_ |
1766343551898091520 |