Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum
Abstract Background The efficacy of anti-malarial drugs is determined by the level of parasite susceptibility, anti-malarial drug bioavailability and pharmacokinetics, and host factors including immunity. Host immunity improves the in vivo therapeutic efficacy of anti-malarial drugs, but the mechani...
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ftdoajarticles:oai:doaj.org/article:54a9f05b414244e3a9876183129d707a 2023-05-15T15:15:49+02:00 Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum Udomsangpetch Rachanee Krudsood Srivicha Dondorp Arjen M Mungthin Mathirut Monatrakul Preeyaporn Wilairatana Polrat White Nicholas J Chotivanich Kesinee 2010-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-326 https://doaj.org/article/54a9f05b414244e3a9876183129d707a EN eng BMC http://www.malariajournal.com/content/9/1/326 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-326 1475-2875 https://doaj.org/article/54a9f05b414244e3a9876183129d707a Malaria Journal, Vol 9, Iss 1, p 326 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-326 2022-12-31T08:47:28Z Abstract Background The efficacy of anti-malarial drugs is determined by the level of parasite susceptibility, anti-malarial drug bioavailability and pharmacokinetics, and host factors including immunity. Host immunity improves the in vivo therapeutic efficacy of anti-malarial drugs, but the mechanism and magnitude of this effect has not been characterized. This study characterized the effects of 'immune' plasma to Plasmodium falciparum on the in vitro susceptibility of P. falciparum to anti-malarial drugs. Methods Titres of antibodies against blood stage antigens (mainly the ring-infected erythrocyte surface antigen [RESA]) were measured in plasma samples obtained from Thai patients with acute falciparum malaria. 'Immune' plasma was selected and its effects on in vitro parasite growth and multiplication of the Thai P. falciparum laboratory strain TM267 were assessed by light microscopy. The in vitro susceptibility to quinine and artesunate was then determined in the presence and absence of 'immune' plasma using the 3 H-hypoxanthine uptake inhibition method. Drug susceptibility was expressed as the concentrations causing 50% and 90% inhibition (IC 50 and IC 90 ), of 3 H-hypoxanthine uptake. Results Incubation with 'immune' plasma reduced parasite maturation and decreased parasite multiplication in a dose dependent manner. 3 H-hypoxanthine incorporation after incubation with 'immune' plasma was decreased significantly compared to controls (median [range]; 181.5 [0 to 3,269] cpm versus 1,222.5 [388 to 5,932] cpm) ( p = 0.001). As a result 'immune' plasma reduced apparent susceptibility to quinine substantially; median (range) IC 50 6.4 (0.5 to 23.8) ng/ml versus 221.5 (174.4 to 250.4) ng/ml ( p = 0.02), and also had a borderline effect on artesunate susceptibility; IC 50 0.2 (0.02 to 0.3) ng/ml versus 0.8 (0.2 to 2.3) ng/ml ( p = 0.08). Effects were greatest at low concentrations, changing the shape of the concentration-effect relationship. IC 90 values were not significantly affected; median (range) IC 90 448.0 ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 326 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Udomsangpetch Rachanee Krudsood Srivicha Dondorp Arjen M Mungthin Mathirut Monatrakul Preeyaporn Wilairatana Polrat White Nicholas J Chotivanich Kesinee Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The efficacy of anti-malarial drugs is determined by the level of parasite susceptibility, anti-malarial drug bioavailability and pharmacokinetics, and host factors including immunity. Host immunity improves the in vivo therapeutic efficacy of anti-malarial drugs, but the mechanism and magnitude of this effect has not been characterized. This study characterized the effects of 'immune' plasma to Plasmodium falciparum on the in vitro susceptibility of P. falciparum to anti-malarial drugs. Methods Titres of antibodies against blood stage antigens (mainly the ring-infected erythrocyte surface antigen [RESA]) were measured in plasma samples obtained from Thai patients with acute falciparum malaria. 'Immune' plasma was selected and its effects on in vitro parasite growth and multiplication of the Thai P. falciparum laboratory strain TM267 were assessed by light microscopy. The in vitro susceptibility to quinine and artesunate was then determined in the presence and absence of 'immune' plasma using the 3 H-hypoxanthine uptake inhibition method. Drug susceptibility was expressed as the concentrations causing 50% and 90% inhibition (IC 50 and IC 90 ), of 3 H-hypoxanthine uptake. Results Incubation with 'immune' plasma reduced parasite maturation and decreased parasite multiplication in a dose dependent manner. 3 H-hypoxanthine incorporation after incubation with 'immune' plasma was decreased significantly compared to controls (median [range]; 181.5 [0 to 3,269] cpm versus 1,222.5 [388 to 5,932] cpm) ( p = 0.001). As a result 'immune' plasma reduced apparent susceptibility to quinine substantially; median (range) IC 50 6.4 (0.5 to 23.8) ng/ml versus 221.5 (174.4 to 250.4) ng/ml ( p = 0.02), and also had a borderline effect on artesunate susceptibility; IC 50 0.2 (0.02 to 0.3) ng/ml versus 0.8 (0.2 to 2.3) ng/ml ( p = 0.08). Effects were greatest at low concentrations, changing the shape of the concentration-effect relationship. IC 90 values were not significantly affected; median (range) IC 90 448.0 ... |
format |
Article in Journal/Newspaper |
author |
Udomsangpetch Rachanee Krudsood Srivicha Dondorp Arjen M Mungthin Mathirut Monatrakul Preeyaporn Wilairatana Polrat White Nicholas J Chotivanich Kesinee |
author_facet |
Udomsangpetch Rachanee Krudsood Srivicha Dondorp Arjen M Mungthin Mathirut Monatrakul Preeyaporn Wilairatana Polrat White Nicholas J Chotivanich Kesinee |
author_sort |
Udomsangpetch Rachanee |
title |
Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
title_short |
Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
title_full |
Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
title_fullStr |
Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
title_full_unstemmed |
Modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium falciparum |
title_sort |
modulating effects of plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in plasmodium falciparum |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-326 https://doaj.org/article/54a9f05b414244e3a9876183129d707a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 326 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/326 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-326 1475-2875 https://doaj.org/article/54a9f05b414244e3a9876183129d707a |
op_doi |
https://doi.org/10.1186/1475-2875-9-326 |
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Malaria Journal |
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9 |
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326 |
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1766346152497643520 |