Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and...
Published in: | MycoKeys |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Pensoft Publishers
2018
|
Subjects: | |
Online Access: | https://doi.org/10.3897/mycokeys.43.29093 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac |
id |
ftdoajarticles:oai:doaj.org/article:53cf87de5f844311a4a1dac72f051bac |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:53cf87de5f844311a4a1dac72f051bac 2023-05-15T13:52:11+02:00 Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra Felix Grewe Elisa Lagostina Huini Wu Christian Printzen H. Thorsten Lumbsch 2018-12-01T00:00:00Z https://doi.org/10.3897/mycokeys.43.29093 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac EN eng Pensoft Publishers https://mycokeys.pensoft.net/article/29093/ https://mycokeys.pensoft.net/article/29093/download/pdf/ https://mycokeys.pensoft.net/article/29093/download/xml/ https://doaj.org/toc/1314-4057 https://doaj.org/toc/1314-4049 doi:10.3897/mycokeys.43.29093 1314-4049 1314-4057 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac MycoKeys, Vol 43, Iss , Pp 91-113 (2018) Botany QK1-989 article 2018 ftdoajarticles https://doi.org/10.3897/mycokeys.43.29093 2022-12-30T23:00:10Z Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses. Article in Journal/Newspaper Antarc* Antarctic Antarctica Usnea antarctica Directory of Open Access Journals: DOAJ Articles Antarctic MycoKeys 43 91 113 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Botany QK1-989 |
spellingShingle |
Botany QK1-989 Felix Grewe Elisa Lagostina Huini Wu Christian Printzen H. Thorsten Lumbsch Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
topic_facet |
Botany QK1-989 |
description |
Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses. |
format |
Article in Journal/Newspaper |
author |
Felix Grewe Elisa Lagostina Huini Wu Christian Printzen H. Thorsten Lumbsch |
author_facet |
Felix Grewe Elisa Lagostina Huini Wu Christian Printzen H. Thorsten Lumbsch |
author_sort |
Felix Grewe |
title |
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
title_short |
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
title_full |
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
title_fullStr |
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
title_full_unstemmed |
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra |
title_sort |
population genomic analyses of rad sequences resolves the phylogenetic relationship of the lichen-forming fungal species usnea antarctica and usnea aurantiacoatra |
publisher |
Pensoft Publishers |
publishDate |
2018 |
url |
https://doi.org/10.3897/mycokeys.43.29093 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Usnea antarctica |
genre_facet |
Antarc* Antarctic Antarctica Usnea antarctica |
op_source |
MycoKeys, Vol 43, Iss , Pp 91-113 (2018) |
op_relation |
https://mycokeys.pensoft.net/article/29093/ https://mycokeys.pensoft.net/article/29093/download/pdf/ https://mycokeys.pensoft.net/article/29093/download/xml/ https://doaj.org/toc/1314-4057 https://doaj.org/toc/1314-4049 doi:10.3897/mycokeys.43.29093 1314-4049 1314-4057 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac |
op_doi |
https://doi.org/10.3897/mycokeys.43.29093 |
container_title |
MycoKeys |
container_volume |
43 |
container_start_page |
91 |
op_container_end_page |
113 |
_version_ |
1766256439701012480 |