Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra

Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and...

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Published in:MycoKeys
Main Authors: Felix Grewe, Elisa Lagostina, Huini Wu, Christian Printzen, H. Thorsten Lumbsch
Format: Article in Journal/Newspaper
Language:English
Published: Pensoft Publishers 2018
Subjects:
Online Access:https://doi.org/10.3897/mycokeys.43.29093
https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac
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spelling ftdoajarticles:oai:doaj.org/article:53cf87de5f844311a4a1dac72f051bac 2023-05-15T13:52:11+02:00 Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra Felix Grewe Elisa Lagostina Huini Wu Christian Printzen H. Thorsten Lumbsch 2018-12-01T00:00:00Z https://doi.org/10.3897/mycokeys.43.29093 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac EN eng Pensoft Publishers https://mycokeys.pensoft.net/article/29093/ https://mycokeys.pensoft.net/article/29093/download/pdf/ https://mycokeys.pensoft.net/article/29093/download/xml/ https://doaj.org/toc/1314-4057 https://doaj.org/toc/1314-4049 doi:10.3897/mycokeys.43.29093 1314-4049 1314-4057 https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac MycoKeys, Vol 43, Iss , Pp 91-113 (2018) Botany QK1-989 article 2018 ftdoajarticles https://doi.org/10.3897/mycokeys.43.29093 2022-12-30T23:00:10Z Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses. Article in Journal/Newspaper Antarc* Antarctic Antarctica Usnea antarctica Directory of Open Access Journals: DOAJ Articles Antarctic MycoKeys 43 91 113
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Botany
QK1-989
spellingShingle Botany
QK1-989
Felix Grewe
Elisa Lagostina
Huini Wu
Christian Printzen
H. Thorsten Lumbsch
Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
topic_facet Botany
QK1-989
description Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses.
format Article in Journal/Newspaper
author Felix Grewe
Elisa Lagostina
Huini Wu
Christian Printzen
H. Thorsten Lumbsch
author_facet Felix Grewe
Elisa Lagostina
Huini Wu
Christian Printzen
H. Thorsten Lumbsch
author_sort Felix Grewe
title Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
title_short Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
title_full Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
title_fullStr Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
title_full_unstemmed Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
title_sort population genomic analyses of rad sequences resolves the phylogenetic relationship of the lichen-forming fungal species usnea antarctica and usnea aurantiacoatra
publisher Pensoft Publishers
publishDate 2018
url https://doi.org/10.3897/mycokeys.43.29093
https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac
geographic Antarctic
geographic_facet Antarctic
genre Antarc*
Antarctic
Antarctica
Usnea antarctica
genre_facet Antarc*
Antarctic
Antarctica
Usnea antarctica
op_source MycoKeys, Vol 43, Iss , Pp 91-113 (2018)
op_relation https://mycokeys.pensoft.net/article/29093/
https://mycokeys.pensoft.net/article/29093/download/pdf/
https://mycokeys.pensoft.net/article/29093/download/xml/
https://doaj.org/toc/1314-4057
https://doaj.org/toc/1314-4049
doi:10.3897/mycokeys.43.29093
1314-4049
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https://doaj.org/article/53cf87de5f844311a4a1dac72f051bac
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container_title MycoKeys
container_volume 43
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