Molecular characterization of Brucella species from Zimbabwe.
Brucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of mol...
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ftdoajarticles:oai:doaj.org/article:53ca14d9a1e646e7b7f039eb98b287c5 2023-05-15T15:07:51+02:00 Molecular characterization of Brucella species from Zimbabwe. Maphuti Betty Ledwaba Calvin Gomo Kgaugelo Edward Lekota Philippe Le Flèche Ayesha Hassim Gilles Vergnaud Henriette van Heerden 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007311 https://doaj.org/article/53ca14d9a1e646e7b7f039eb98b287c5 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007311 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007311 https://doaj.org/article/53ca14d9a1e646e7b7f039eb98b287c5 PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007311 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007311 2022-12-31T05:04:49Z Brucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of molecular assays including AMOS-PCR, Bruce-ladder, multiple locus variable number tandem repeats analysis (MLVA) and single nucleotide polymorphisms from whole genome sequencing (WGS-SNP) to characterize Brucella isolates to the species, biovar, and individual strain level. Sixteen Brucella strains isolated in Zimbabwe at the Central Veterinary laboratory from various hosts were characterized using all or some of these assays. The strains were identified as B. ovis, B. abortus, B. canis and B. suis, with B. canis being the first report of this species in Zimbabwe. Zimbabwean strains identified as B. suis and B. abortus were further characterized with whole genome sequencing and were closely related to reference strains 1330 and 86/8/59, respectively. We demonstrate the range of different tests that can be performed from simple assays that can be run in laboratories lacking sophisticated instrumentation to whole genome analyses that currently require substantial expertise and infrastructure often not available in the developing world. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007311 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Maphuti Betty Ledwaba Calvin Gomo Kgaugelo Edward Lekota Philippe Le Flèche Ayesha Hassim Gilles Vergnaud Henriette van Heerden Molecular characterization of Brucella species from Zimbabwe. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Brucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of molecular assays including AMOS-PCR, Bruce-ladder, multiple locus variable number tandem repeats analysis (MLVA) and single nucleotide polymorphisms from whole genome sequencing (WGS-SNP) to characterize Brucella isolates to the species, biovar, and individual strain level. Sixteen Brucella strains isolated in Zimbabwe at the Central Veterinary laboratory from various hosts were characterized using all or some of these assays. The strains were identified as B. ovis, B. abortus, B. canis and B. suis, with B. canis being the first report of this species in Zimbabwe. Zimbabwean strains identified as B. suis and B. abortus were further characterized with whole genome sequencing and were closely related to reference strains 1330 and 86/8/59, respectively. We demonstrate the range of different tests that can be performed from simple assays that can be run in laboratories lacking sophisticated instrumentation to whole genome analyses that currently require substantial expertise and infrastructure often not available in the developing world. |
format |
Article in Journal/Newspaper |
author |
Maphuti Betty Ledwaba Calvin Gomo Kgaugelo Edward Lekota Philippe Le Flèche Ayesha Hassim Gilles Vergnaud Henriette van Heerden |
author_facet |
Maphuti Betty Ledwaba Calvin Gomo Kgaugelo Edward Lekota Philippe Le Flèche Ayesha Hassim Gilles Vergnaud Henriette van Heerden |
author_sort |
Maphuti Betty Ledwaba |
title |
Molecular characterization of Brucella species from Zimbabwe. |
title_short |
Molecular characterization of Brucella species from Zimbabwe. |
title_full |
Molecular characterization of Brucella species from Zimbabwe. |
title_fullStr |
Molecular characterization of Brucella species from Zimbabwe. |
title_full_unstemmed |
Molecular characterization of Brucella species from Zimbabwe. |
title_sort |
molecular characterization of brucella species from zimbabwe. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007311 https://doaj.org/article/53ca14d9a1e646e7b7f039eb98b287c5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007311 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007311 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007311 https://doaj.org/article/53ca14d9a1e646e7b7f039eb98b287c5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007311 |
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PLOS Neglected Tropical Diseases |
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13 |
container_issue |
5 |
container_start_page |
e0007311 |
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1766339270483640320 |