Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene
Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined with the nested PCR method by using BToV M gene-specific primers. Results: BToV...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:53a9255abb594fe2a471af0ce95b7be0 2023-05-15T15:09:03+02:00 Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene Hakan Aydin Mehmet Ozkan Timurkan Gulizar Acar Kirmizi 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.254607 https://doaj.org/article/53a9255abb594fe2a471af0ce95b7be0 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=129;epage=134;aulast=Aydin https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254607 https://doaj.org/article/53a9255abb594fe2a471af0ce95b7be0 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 129-134 (2019) calf diarrhea torovirus turkey Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.254607 2022-12-31T03:32:58Z Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined with the nested PCR method by using BToV M gene-specific primers. Results: BToV was detected in 12 (16.7%) out of 72 samples. Phylogenetic analysis was performed using nucleotide and amino acid sequences. In the phylogenetic tree, European, American, Far East and Turkish strains were found to be divided into different branches. Interestingly, it was observed that Turkish strains were divided into two subgroups. Considering the amino acid sequences of these strains having differences at nucleotide level, the change at the 3rd amino acid of the partial M gene in Turkish strains has made Turkish strains different from all other strains. Similarly, the differences were observed in the 18th, 20th, 63rd and 93rd amino acids of the partial M gene only in Turkish field strains. Conclusions: This study revealed that Turkish strains of BToV constitute a separate phylogenetic group and can be divided into two subgroups. In addition, BToV was found to be a common pathogen causing diarrhea in calves in Turkey, and it is a necessity to consider BToV in cases of diarrhea with unknown cause. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 3 129 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
calf diarrhea torovirus turkey Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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calf diarrhea torovirus turkey Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Hakan Aydin Mehmet Ozkan Timurkan Gulizar Acar Kirmizi Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
topic_facet |
calf diarrhea torovirus turkey Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined with the nested PCR method by using BToV M gene-specific primers. Results: BToV was detected in 12 (16.7%) out of 72 samples. Phylogenetic analysis was performed using nucleotide and amino acid sequences. In the phylogenetic tree, European, American, Far East and Turkish strains were found to be divided into different branches. Interestingly, it was observed that Turkish strains were divided into two subgroups. Considering the amino acid sequences of these strains having differences at nucleotide level, the change at the 3rd amino acid of the partial M gene in Turkish strains has made Turkish strains different from all other strains. Similarly, the differences were observed in the 18th, 20th, 63rd and 93rd amino acids of the partial M gene only in Turkish field strains. Conclusions: This study revealed that Turkish strains of BToV constitute a separate phylogenetic group and can be divided into two subgroups. In addition, BToV was found to be a common pathogen causing diarrhea in calves in Turkey, and it is a necessity to consider BToV in cases of diarrhea with unknown cause. |
format |
Article in Journal/Newspaper |
author |
Hakan Aydin Mehmet Ozkan Timurkan Gulizar Acar Kirmizi |
author_facet |
Hakan Aydin Mehmet Ozkan Timurkan Gulizar Acar Kirmizi |
author_sort |
Hakan Aydin |
title |
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
title_short |
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
title_full |
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
title_fullStr |
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
title_full_unstemmed |
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene |
title_sort |
sequence analysis of turkish field strains of bovine torovirus shows unique amino acid changes in the partial m gene |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.254607 https://doaj.org/article/53a9255abb594fe2a471af0ce95b7be0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 129-134 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=129;epage=134;aulast=Aydin https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254607 https://doaj.org/article/53a9255abb594fe2a471af0ce95b7be0 |
op_doi |
https://doi.org/10.4103/2221-1691.254607 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
3 |
container_start_page |
129 |
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1766340285386719232 |