Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains
The decrease in susceptibility to glycopeptides by oxacillin resistant Staphylococcus aureus (ORSA) has become a current problem. Objective: To evaluate susceptibility to glycopeptides in ORSA strains isolated in the University Hospital, Maracaibo, during the first quarter of 2010. Methods: Oxacilli...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2012
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ftdoajarticles:oai:doaj.org/article:53715e1b196f43709cb25d0943736e12 2023-10-09T21:49:11+02:00 Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains Maribel Castellano González Armindo Perozo Mena Ana González 2012-01-01T00:00:00Z https://doaj.org/article/53715e1b196f43709cb25d0943736e12 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4887 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/53715e1b196f43709cb25d0943736e12 Kasmera, Vol 40, Iss 1, Pp 11-22 (2012) SAOR glicopéptidos resistencia Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles 2023-09-10T00:49:12Z The decrease in susceptibility to glycopeptides by oxacillin resistant Staphylococcus aureus (ORSA) has become a current problem. Objective: To evaluate susceptibility to glycopeptides in ORSA strains isolated in the University Hospital, Maracaibo, during the first quarter of 2010. Methods: Oxacillin resistance of the ORSA strains was verified by diffusion on agar (oxacillin 1 μg and cefoxitin 30 μg, OXOID®), a screening test in Müeller Hinton agar with 6 μg/mL of vancomycin (Sigma®), and E-test® for minimum inhibitory concentration (MIC) for oxacillin, detection of PBP2a (Latex, OXOID®) and the mecA gene by polymerase chain reaction (PCR). In addition, susceptibility to glycopeptides was determined by disk diffusion (teicoplanin, 30 μg OXOID®), MIC by E-test®, screening test in Müeller- Hinton agar with teicoplanin (5 μg/ml) or in brain heart infusion agar with vancomycin (6 μg/mL), glycopeptide hetero-resistance detection by GDR E-test® and detection of the vanA gene using PCR. Results: No ORSA strain was isolated with intermediate resistance (VISA or hVISA), nor with high-level resistance to glycopeptides. Conclusions: Locally, there is no incidence of ORSA strains resistant to glycopeptides; therefore, these antibiotics continue to be useful for treating infections produced by this important pathogen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English Spanish |
topic |
SAOR glicopéptidos resistencia Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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SAOR glicopéptidos resistencia Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Maribel Castellano González Armindo Perozo Mena Ana González Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
topic_facet |
SAOR glicopéptidos resistencia Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The decrease in susceptibility to glycopeptides by oxacillin resistant Staphylococcus aureus (ORSA) has become a current problem. Objective: To evaluate susceptibility to glycopeptides in ORSA strains isolated in the University Hospital, Maracaibo, during the first quarter of 2010. Methods: Oxacillin resistance of the ORSA strains was verified by diffusion on agar (oxacillin 1 μg and cefoxitin 30 μg, OXOID®), a screening test in Müeller Hinton agar with 6 μg/mL of vancomycin (Sigma®), and E-test® for minimum inhibitory concentration (MIC) for oxacillin, detection of PBP2a (Latex, OXOID®) and the mecA gene by polymerase chain reaction (PCR). In addition, susceptibility to glycopeptides was determined by disk diffusion (teicoplanin, 30 μg OXOID®), MIC by E-test®, screening test in Müeller- Hinton agar with teicoplanin (5 μg/ml) or in brain heart infusion agar with vancomycin (6 μg/mL), glycopeptide hetero-resistance detection by GDR E-test® and detection of the vanA gene using PCR. Results: No ORSA strain was isolated with intermediate resistance (VISA or hVISA), nor with high-level resistance to glycopeptides. Conclusions: Locally, there is no incidence of ORSA strains resistant to glycopeptides; therefore, these antibiotics continue to be useful for treating infections produced by this important pathogen. |
format |
Article in Journal/Newspaper |
author |
Maribel Castellano González Armindo Perozo Mena Ana González |
author_facet |
Maribel Castellano González Armindo Perozo Mena Ana González |
author_sort |
Maribel Castellano González |
title |
Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
title_short |
Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
title_full |
Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
title_fullStr |
Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
title_full_unstemmed |
Evaluation of Susceptibility to Glycopeptides in Oxacillin Resistant Staphylococcus aureus Strains |
title_sort |
evaluation of susceptibility to glycopeptides in oxacillin resistant staphylococcus aureus strains |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2012 |
url |
https://doaj.org/article/53715e1b196f43709cb25d0943736e12 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 40, Iss 1, Pp 11-22 (2012) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4887 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/53715e1b196f43709cb25d0943736e12 |
_version_ |
1779312209147461632 |