Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand
Objective: To investigate the prevalence of cercarial trematode infection in snails and to examine the reconstruction of the phylogenetic relationship to explain the molecular system of cercarial stage trematodes to estimate the infection rate of in the definite host from the Chao-Phraya Basin. Meth...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:535d641271c9455cae8febc3b202799e 2023-05-15T15:11:59+02:00 Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand Sothorn Anucherngchai Thanawan Tejangkura Thapana Chontananarth 2016-06-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.01.015 https://doaj.org/article/535d641271c9455cae8febc3b202799e EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S222116911630288X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.01.015 https://doaj.org/article/535d641271c9455cae8febc3b202799e Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 6, Pp 539-545 (2016) Prevalence Molecular identification Cercarial infection Freshwater snails Thailand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.01.015 2022-12-31T07:25:59Z Objective: To investigate the prevalence of cercarial trematode infection in snails and to examine the reconstruction of the phylogenetic relationship to explain the molecular system of cercarial stage trematodes to estimate the infection rate of in the definite host from the Chao-Phraya Basin. Methods: The snails were collected from 10 provinces of the Chao-Phraya Basin, Thailand by stratified sampling method. The snails were examined for cercarial infection by the crushing method. All DNA specimens were amplified with internal transcribed spacer 3 (ITS3) and ITS4 primer based on PCR technique. The sequence data were aligned and used to reconstruct the phylogenetic tree by unweighted pair-group method with arithmetic means with 10000 bootstraps. Results: The overall rate of cercarial infection was found to be 5.90% (122/2067). Snails in the family Thiaridae were found to be in the highest prevalence followed by Lymnaeidae, Bithyniidae, Planorbidae, Viviparidae, and Ampullariidae, respectively, while the Buccinidae family (Clea helena) did not reveal any infections. The frequently found species of cercariae were parapleurolophocercous cercariae, cercariae and megarulous cercariae. The monophyletic tree separated the snails into five groups comprised of Heterophyidae, Strigeidae, Lecithodendriidae, Philophthalmidae and Echinostomatidae using the sequence of Angiostrongylus cantonensis as an out-group. Conclusions: This study was the first to report on cercarial infection in the Chao-Phraya Basin, Thailand. This revealed that a high variety of freshwater snails were infected by cercariae stage trematodes with a high prevalence. The sequence data of ITS2 can be used to investigate the phylogenetic relationships of trematodes at the family level and in each clade of different families separated by the definitive hosts. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 6 539 545 |
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Prevalence Molecular identification Cercarial infection Freshwater snails Thailand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Prevalence Molecular identification Cercarial infection Freshwater snails Thailand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sothorn Anucherngchai Thanawan Tejangkura Thapana Chontananarth Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
topic_facet |
Prevalence Molecular identification Cercarial infection Freshwater snails Thailand Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the prevalence of cercarial trematode infection in snails and to examine the reconstruction of the phylogenetic relationship to explain the molecular system of cercarial stage trematodes to estimate the infection rate of in the definite host from the Chao-Phraya Basin. Methods: The snails were collected from 10 provinces of the Chao-Phraya Basin, Thailand by stratified sampling method. The snails were examined for cercarial infection by the crushing method. All DNA specimens were amplified with internal transcribed spacer 3 (ITS3) and ITS4 primer based on PCR technique. The sequence data were aligned and used to reconstruct the phylogenetic tree by unweighted pair-group method with arithmetic means with 10000 bootstraps. Results: The overall rate of cercarial infection was found to be 5.90% (122/2067). Snails in the family Thiaridae were found to be in the highest prevalence followed by Lymnaeidae, Bithyniidae, Planorbidae, Viviparidae, and Ampullariidae, respectively, while the Buccinidae family (Clea helena) did not reveal any infections. The frequently found species of cercariae were parapleurolophocercous cercariae, cercariae and megarulous cercariae. The monophyletic tree separated the snails into five groups comprised of Heterophyidae, Strigeidae, Lecithodendriidae, Philophthalmidae and Echinostomatidae using the sequence of Angiostrongylus cantonensis as an out-group. Conclusions: This study was the first to report on cercarial infection in the Chao-Phraya Basin, Thailand. This revealed that a high variety of freshwater snails were infected by cercariae stage trematodes with a high prevalence. The sequence data of ITS2 can be used to investigate the phylogenetic relationships of trematodes at the family level and in each clade of different families separated by the definitive hosts. |
format |
Article in Journal/Newspaper |
author |
Sothorn Anucherngchai Thanawan Tejangkura Thapana Chontananarth |
author_facet |
Sothorn Anucherngchai Thanawan Tejangkura Thapana Chontananarth |
author_sort |
Sothorn Anucherngchai |
title |
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
title_short |
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
title_full |
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
title_fullStr |
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
title_full_unstemmed |
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand |
title_sort |
epidemiological situation and molecular identification of cercarial stage in freshwater snails in chao-phraya basin, central thailand |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.01.015 https://doaj.org/article/535d641271c9455cae8febc3b202799e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 6, Pp 539-545 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S222116911630288X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.01.015 https://doaj.org/article/535d641271c9455cae8febc3b202799e |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.01.015 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
6 |
container_start_page |
539 |
op_container_end_page |
545 |
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1766342745944752128 |