Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates
Abstract Background Plasmodium falciparum apical membrane antigen-1 (PfAMA-1) is one of leading blood stage malaria vaccine candidates. However, genetic variation and antigenic diversity identified in global PfAMA-1 are major hurdles in the development of an effective vaccine based on this antigen....
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ftdoajarticles:oai:doaj.org/article:5343da37153648eeb316f8d33bd289c0 2023-05-15T15:18:14+02:00 Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates Jung-Mi Kang Jinyoung Lee Mya Moe Hojong Jun Hương Giang Lê Tae Im Kim Thị Lam Thái Woon-Mok Sohn Moe Kyaw Myint Khin Lin Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na 2018-02-01T00:00:00Z https://doi.org/10.1186/s12936-018-2215-7 https://doaj.org/article/5343da37153648eeb316f8d33bd289c0 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2215-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2215-7 1475-2875 https://doaj.org/article/5343da37153648eeb316f8d33bd289c0 Malaria Journal, Vol 17, Iss 1, Pp 1-17 (2018) Plasmodium falciparum Apical membrane antigen-1 Genetic diversity Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2215-7 2022-12-31T03:44:41Z Abstract Background Plasmodium falciparum apical membrane antigen-1 (PfAMA-1) is one of leading blood stage malaria vaccine candidates. However, genetic variation and antigenic diversity identified in global PfAMA-1 are major hurdles in the development of an effective vaccine based on this antigen. In this study, genetic structure and the effect of natural selection of PfAMA-1 among Myanmar P. falciparum isolates were analysed. Methods Blood samples were collected from 58 Myanmar patients with falciparum malaria. Full-length PfAMA-1 gene was amplified by polymerase chain reaction and cloned into a TA cloning vector. PfAMA-1 sequence of each isolate was sequenced. Polymorphic characteristics and effect of natural selection were analysed with using DNASTAR, MEGA4, and DnaSP programs. Polymorphic nature and natural selection in 459 global PfAMA-1 were also analysed. Results Thirty-seven different haplotypes of PfAMA-1 were identified in 58 Myanmar P. falciparum isolates. Most amino acid changes identified in Myanmar PfAMA-1 were found in domains I and III. Overall patterns of amino acid changes in Myanmar PfAMA-1 were similar to those in global PfAMA-1. However, frequencies of amino acid changes differed by country. Novel amino acid changes in Myanmar PfAMA-1 were also identified. Evidences for natural selection and recombination event were observed in global PfAMA-1. Among 51 commonly identified amino acid changes in global PfAMA-1 sequences, 43 were found in predicted RBC-binding sites, B-cell epitopes, or IUR regions. Conclusions Myanmar PfAMA-1 showed similar patterns of nucleotide diversity and amino acid polymorphisms compared to those of global PfAMA-1. Balancing natural selection and intragenic recombination across PfAMA-1 are likely to play major roles in generating genetic diversity in global PfAMA-1. Most common amino acid changes in global PfAMA-1 were located in predicted B-cell epitopes where high levels of nucleotide diversity and balancing natural selection were found. These results highlight the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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topic |
Plasmodium falciparum Apical membrane antigen-1 Genetic diversity Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum Apical membrane antigen-1 Genetic diversity Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jung-Mi Kang Jinyoung Lee Mya Moe Hojong Jun Hương Giang Lê Tae Im Kim Thị Lam Thái Woon-Mok Sohn Moe Kyaw Myint Khin Lin Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
topic_facet |
Plasmodium falciparum Apical membrane antigen-1 Genetic diversity Natural selection Myanmar Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum apical membrane antigen-1 (PfAMA-1) is one of leading blood stage malaria vaccine candidates. However, genetic variation and antigenic diversity identified in global PfAMA-1 are major hurdles in the development of an effective vaccine based on this antigen. In this study, genetic structure and the effect of natural selection of PfAMA-1 among Myanmar P. falciparum isolates were analysed. Methods Blood samples were collected from 58 Myanmar patients with falciparum malaria. Full-length PfAMA-1 gene was amplified by polymerase chain reaction and cloned into a TA cloning vector. PfAMA-1 sequence of each isolate was sequenced. Polymorphic characteristics and effect of natural selection were analysed with using DNASTAR, MEGA4, and DnaSP programs. Polymorphic nature and natural selection in 459 global PfAMA-1 were also analysed. Results Thirty-seven different haplotypes of PfAMA-1 were identified in 58 Myanmar P. falciparum isolates. Most amino acid changes identified in Myanmar PfAMA-1 were found in domains I and III. Overall patterns of amino acid changes in Myanmar PfAMA-1 were similar to those in global PfAMA-1. However, frequencies of amino acid changes differed by country. Novel amino acid changes in Myanmar PfAMA-1 were also identified. Evidences for natural selection and recombination event were observed in global PfAMA-1. Among 51 commonly identified amino acid changes in global PfAMA-1 sequences, 43 were found in predicted RBC-binding sites, B-cell epitopes, or IUR regions. Conclusions Myanmar PfAMA-1 showed similar patterns of nucleotide diversity and amino acid polymorphisms compared to those of global PfAMA-1. Balancing natural selection and intragenic recombination across PfAMA-1 are likely to play major roles in generating genetic diversity in global PfAMA-1. Most common amino acid changes in global PfAMA-1 were located in predicted B-cell epitopes where high levels of nucleotide diversity and balancing natural selection were found. These results highlight the ... |
format |
Article in Journal/Newspaper |
author |
Jung-Mi Kang Jinyoung Lee Mya Moe Hojong Jun Hương Giang Lê Tae Im Kim Thị Lam Thái Woon-Mok Sohn Moe Kyaw Myint Khin Lin Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na |
author_facet |
Jung-Mi Kang Jinyoung Lee Mya Moe Hojong Jun Hương Giang Lê Tae Im Kim Thị Lam Thái Woon-Mok Sohn Moe Kyaw Myint Khin Lin Ho-Joon Shin Tong-Soo Kim Byoung-Kuk Na |
author_sort |
Jung-Mi Kang |
title |
Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
title_short |
Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
title_full |
Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
title_fullStr |
Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
title_full_unstemmed |
Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates |
title_sort |
population genetic structure and natural selection of plasmodium falciparum apical membrane antigen-1 in myanmar isolates |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2215-7 https://doaj.org/article/5343da37153648eeb316f8d33bd289c0 |
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Arctic |
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Arctic |
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Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-17 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2215-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2215-7 1475-2875 https://doaj.org/article/5343da37153648eeb316f8d33bd289c0 |
op_doi |
https://doi.org/10.1186/s12936-018-2215-7 |
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Malaria Journal |
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17 |
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1766348445951459328 |