Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production.
One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-γ (IFN-γ). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to pro...
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ftdoajarticles:oai:doaj.org/article:5313c0b6fcff41ee9f16ed5fff478c22 2023-05-15T15:11:29+02:00 Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. Emebet Adem Fitsumbirhan Tajebe Mulusew Getahun Amare Kiflie Ermias Diro Asrat Hailu Ziv Shkedy Bewketu Mengesha Tadele Mulaw Saba Atnafu Tekalign Deressa Biniam Mathewos Ebba Abate Manuel Modolell Markus Munder Ingrid Müller Yegnasew Takele Pascale Kropf 2016-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004468 https://doaj.org/article/5313c0b6fcff41ee9f16ed5fff478c22 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4786308?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004468 https://doaj.org/article/5313c0b6fcff41ee9f16ed5fff478c22 PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004468 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004468 2022-12-31T02:27:06Z One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-γ (IFN-γ). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-γ. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-γ and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-γ and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-γ, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-γ during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 3 e0004468 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Emebet Adem Fitsumbirhan Tajebe Mulusew Getahun Amare Kiflie Ermias Diro Asrat Hailu Ziv Shkedy Bewketu Mengesha Tadele Mulaw Saba Atnafu Tekalign Deressa Biniam Mathewos Ebba Abate Manuel Modolell Markus Munder Ingrid Müller Yegnasew Takele Pascale Kropf Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-γ (IFN-γ). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-γ. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-γ and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-γ and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-γ, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-γ during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation. |
format |
Article in Journal/Newspaper |
author |
Emebet Adem Fitsumbirhan Tajebe Mulusew Getahun Amare Kiflie Ermias Diro Asrat Hailu Ziv Shkedy Bewketu Mengesha Tadele Mulaw Saba Atnafu Tekalign Deressa Biniam Mathewos Ebba Abate Manuel Modolell Markus Munder Ingrid Müller Yegnasew Takele Pascale Kropf |
author_facet |
Emebet Adem Fitsumbirhan Tajebe Mulusew Getahun Amare Kiflie Ermias Diro Asrat Hailu Ziv Shkedy Bewketu Mengesha Tadele Mulaw Saba Atnafu Tekalign Deressa Biniam Mathewos Ebba Abate Manuel Modolell Markus Munder Ingrid Müller Yegnasew Takele Pascale Kropf |
author_sort |
Emebet Adem |
title |
Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
title_short |
Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
title_full |
Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
title_fullStr |
Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
title_full_unstemmed |
Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production. |
title_sort |
successful treatment of human visceral leishmaniasis restores antigen-specific ifn-γ, but not il-10 production. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004468 https://doaj.org/article/5313c0b6fcff41ee9f16ed5fff478c22 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004468 (2016) |
op_relation |
http://europepmc.org/articles/PMC4786308?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004468 https://doaj.org/article/5313c0b6fcff41ee9f16ed5fff478c22 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004468 |
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PLOS Neglected Tropical Diseases |
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3 |
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e0004468 |
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