Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico.
Arbovirus infection in Aedes aegypti has historically been quantified from a sample of the adult population by pooling collected mosquitoes to increase detectability. However, there is a significant knowledge gap about the magnitude of natural arbovirus infection within areas of active transmission,...
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ftdoajarticles:oai:doaj.org/article:52fe3163506f41269e6ab3465aebbc3a 2023-05-15T15:15:45+02:00 Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. Oscar David Kirstein Guadalupe Ayora-Talavera Edgar Koyoc-Cardeña Daniel Chan Espinoza Azael Che-Mendoza Azael Cohuo-Rodriguez Pilar Granja-Pérez Henry Puerta-Guardo Norma Pavia-Ruz Mike W Dunbar Pablo Manrique-Saide Gonzalo M Vazquez-Prokopec 2021-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008972 https://doaj.org/article/52fe3163506f41269e6ab3465aebbc3a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008972 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008972 https://doaj.org/article/52fe3163506f41269e6ab3465aebbc3a PLoS Neglected Tropical Diseases, Vol 15, Iss 1, p e0008972 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008972 2022-12-31T05:07:34Z Arbovirus infection in Aedes aegypti has historically been quantified from a sample of the adult population by pooling collected mosquitoes to increase detectability. However, there is a significant knowledge gap about the magnitude of natural arbovirus infection within areas of active transmission, as well as the sensitivity of detection of such an approach. We used indoor Ae. aegypti sequential sampling with Prokopack aspirators to collect all mosquitoes inside 200 houses with suspected active ABV transmission from the city of Mérida, Mexico, and tested all collected specimens by RT-PCR to quantify: a) the absolute arbovirus infection rate in individually tested Ae. aegypti females; b) the sensitivity of using Prokopack aspirators in detecting ABV-infected mosquitoes; and c) the sensitivity of entomological inoculation rate (EIR) and vectorial capacity (VC), two measures ABV transmission potential, to different estimates of indoor Ae. aegypti abundance. The total number of Ae. aegypti (total catch, the sum of all Ae. aegypti across all collection intervals) as well as the number on the first 10-min of collection (sample, equivalent to a routine adult aspiration session) were calculated. We individually tested by RT-PCR 2,161 Aedes aegypti females and found that 7.7% of them were positive to any ABV. Most infections were CHIKV (77.7%), followed by DENV (11.4%) and ZIKV (9.0%). The distribution of infected Aedes aegypti was overdispersed; 33% houses contributed 81% of the infected mosquitoes. A significant association between ABV infection and Ae. aegypti total catch indoors was found (binomial GLMM, Odds Ratio > 1). A 10-min indoor Prokopack collection led to a low sensitivity of detecting ABV infection (16.3% for detecting infected mosquitoes and 23.4% for detecting infected houses). When averaged across all infested houses, mean EIR ranged between 0.04 and 0.06 infective bites per person per day, and mean VC was 0.6 infectious vectors generated from a population feeding on a single infected host per ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 1 e0008972 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Oscar David Kirstein Guadalupe Ayora-Talavera Edgar Koyoc-Cardeña Daniel Chan Espinoza Azael Che-Mendoza Azael Cohuo-Rodriguez Pilar Granja-Pérez Henry Puerta-Guardo Norma Pavia-Ruz Mike W Dunbar Pablo Manrique-Saide Gonzalo M Vazquez-Prokopec Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Arbovirus infection in Aedes aegypti has historically been quantified from a sample of the adult population by pooling collected mosquitoes to increase detectability. However, there is a significant knowledge gap about the magnitude of natural arbovirus infection within areas of active transmission, as well as the sensitivity of detection of such an approach. We used indoor Ae. aegypti sequential sampling with Prokopack aspirators to collect all mosquitoes inside 200 houses with suspected active ABV transmission from the city of Mérida, Mexico, and tested all collected specimens by RT-PCR to quantify: a) the absolute arbovirus infection rate in individually tested Ae. aegypti females; b) the sensitivity of using Prokopack aspirators in detecting ABV-infected mosquitoes; and c) the sensitivity of entomological inoculation rate (EIR) and vectorial capacity (VC), two measures ABV transmission potential, to different estimates of indoor Ae. aegypti abundance. The total number of Ae. aegypti (total catch, the sum of all Ae. aegypti across all collection intervals) as well as the number on the first 10-min of collection (sample, equivalent to a routine adult aspiration session) were calculated. We individually tested by RT-PCR 2,161 Aedes aegypti females and found that 7.7% of them were positive to any ABV. Most infections were CHIKV (77.7%), followed by DENV (11.4%) and ZIKV (9.0%). The distribution of infected Aedes aegypti was overdispersed; 33% houses contributed 81% of the infected mosquitoes. A significant association between ABV infection and Ae. aegypti total catch indoors was found (binomial GLMM, Odds Ratio > 1). A 10-min indoor Prokopack collection led to a low sensitivity of detecting ABV infection (16.3% for detecting infected mosquitoes and 23.4% for detecting infected houses). When averaged across all infested houses, mean EIR ranged between 0.04 and 0.06 infective bites per person per day, and mean VC was 0.6 infectious vectors generated from a population feeding on a single infected host per ... |
format |
Article in Journal/Newspaper |
author |
Oscar David Kirstein Guadalupe Ayora-Talavera Edgar Koyoc-Cardeña Daniel Chan Espinoza Azael Che-Mendoza Azael Cohuo-Rodriguez Pilar Granja-Pérez Henry Puerta-Guardo Norma Pavia-Ruz Mike W Dunbar Pablo Manrique-Saide Gonzalo M Vazquez-Prokopec |
author_facet |
Oscar David Kirstein Guadalupe Ayora-Talavera Edgar Koyoc-Cardeña Daniel Chan Espinoza Azael Che-Mendoza Azael Cohuo-Rodriguez Pilar Granja-Pérez Henry Puerta-Guardo Norma Pavia-Ruz Mike W Dunbar Pablo Manrique-Saide Gonzalo M Vazquez-Prokopec |
author_sort |
Oscar David Kirstein |
title |
Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
title_short |
Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
title_full |
Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
title_fullStr |
Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
title_full_unstemmed |
Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from Mérida, Yucatán, Mexico. |
title_sort |
natural arbovirus infection rate and detectability of indoor female aedes aegypti from mérida, yucatán, mexico. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008972 https://doaj.org/article/52fe3163506f41269e6ab3465aebbc3a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 1, p e0008972 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008972 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008972 https://doaj.org/article/52fe3163506f41269e6ab3465aebbc3a |
op_doi |
https://doi.org/10.1371/journal.pntd.0008972 |
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PLOS Neglected Tropical Diseases |
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e0008972 |
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