Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease
id="st1" To explore which pattern recognition receptors (PRRs) play a key role in the development of hand, foot, and mouth disease (HFMD) by analyzing PRR-associated genes. id="st2" We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mo...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2024
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Online Access: | https://doi.org/10.4103/apjtm.apjtm_876_23 https://doaj.org/article/52dbdd437d8d48dfb564a0aa9f0a3a53 |
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ftdoajarticles:oai:doaj.org/article:52dbdd437d8d48dfb564a0aa9f0a3a53 2024-09-09T19:27:55+00:00 Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease Muqi Wang Huiling Deng Yuan Chen Yikai Wang Yufeng Zhang Chenrui Liu Meng Zhang Ting Li Shuangsuo Dang Yaping Li 2024-04-01T00:00:00Z https://doi.org/10.4103/apjtm.apjtm_876_23 https://doaj.org/article/52dbdd437d8d48dfb564a0aa9f0a3a53 EN eng Wolters Kluwer Medknow Publications https://journals.lww.com/10.4103/apjtm.apjtm_876_23 https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/apjtm.apjtm_876_23 https://doaj.org/article/52dbdd437d8d48dfb564a0aa9f0a3a53 Asian Pacific Journal of Tropical Medicine, Vol 17, Iss 4, Pp 173-183 (2024) pattern recognition receptors (prrs) hand foot and mouth disease (hfmd) immune enterovirus 71 (ev-a71) Arctic medicine. Tropical medicine RC955-962 article 2024 ftdoajarticles https://doi.org/10.4103/apjtm.apjtm_876_23 2024-08-05T17:49:09Z id="st1" To explore which pattern recognition receptors (PRRs) play a key role in the development of hand, foot, and mouth disease (HFMD) by analyzing PRR-associated genes. id="st2" We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells (PBMCs) infected with enterovirus 71 (EV-A71) which were derived from patients with HFMD of different severities and at different stages. A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates. Subsequently, ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes, specifically, BST2, IRF7, 1FI16, TRIM21, MX1, and DDX58. id="st3" Compared with those at the recovery stage, the expression levels of BST2 (P=0.027), IFI16 (P=0.016), MX1 (P=0.046) and DDX58 (P=0.008) in the acute stage of infection were significantly upregulated, while no significant difference in the expression levels of IRF7 (P=0.495) and TRIM21 (P=0.071) was found between different stages of the disease. The expression levels of BST2, IRF7, IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens, and BST2, IRF7, IFI16 and MX1 expression levels were significantly lower in coxsackie B virus (COXB) positive patients than the negative patients. Expression levels of one or more of BST2, IRF7, IFI16, TRIM21, MX1 and DDX58 genes were correlated with PCT levels, various white blood cell counts, and serum antibody levels that reflect disease course of HFMD. Aspartate aminotransferase was correlated with BST2, MX1 and DDX58 expression levels. id="st4" PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 17 4 173 183 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pattern recognition receptors (prrs) hand foot and mouth disease (hfmd) immune enterovirus 71 (ev-a71) Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
pattern recognition receptors (prrs) hand foot and mouth disease (hfmd) immune enterovirus 71 (ev-a71) Arctic medicine. Tropical medicine RC955-962 Muqi Wang Huiling Deng Yuan Chen Yikai Wang Yufeng Zhang Chenrui Liu Meng Zhang Ting Li Shuangsuo Dang Yaping Li Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
topic_facet |
pattern recognition receptors (prrs) hand foot and mouth disease (hfmd) immune enterovirus 71 (ev-a71) Arctic medicine. Tropical medicine RC955-962 |
description |
id="st1" To explore which pattern recognition receptors (PRRs) play a key role in the development of hand, foot, and mouth disease (HFMD) by analyzing PRR-associated genes. id="st2" We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells (PBMCs) infected with enterovirus 71 (EV-A71) which were derived from patients with HFMD of different severities and at different stages. A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates. Subsequently, ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes, specifically, BST2, IRF7, 1FI16, TRIM21, MX1, and DDX58. id="st3" Compared with those at the recovery stage, the expression levels of BST2 (P=0.027), IFI16 (P=0.016), MX1 (P=0.046) and DDX58 (P=0.008) in the acute stage of infection were significantly upregulated, while no significant difference in the expression levels of IRF7 (P=0.495) and TRIM21 (P=0.071) was found between different stages of the disease. The expression levels of BST2, IRF7, IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens, and BST2, IRF7, IFI16 and MX1 expression levels were significantly lower in coxsackie B virus (COXB) positive patients than the negative patients. Expression levels of one or more of BST2, IRF7, IFI16, TRIM21, MX1 and DDX58 genes were correlated with PCT levels, various white blood cell counts, and serum antibody levels that reflect disease course of HFMD. Aspartate aminotransferase was correlated with BST2, MX1 and DDX58 expression levels. id="st4" PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD. |
format |
Article in Journal/Newspaper |
author |
Muqi Wang Huiling Deng Yuan Chen Yikai Wang Yufeng Zhang Chenrui Liu Meng Zhang Ting Li Shuangsuo Dang Yaping Li |
author_facet |
Muqi Wang Huiling Deng Yuan Chen Yikai Wang Yufeng Zhang Chenrui Liu Meng Zhang Ting Li Shuangsuo Dang Yaping Li |
author_sort |
Muqi Wang |
title |
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
title_short |
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
title_full |
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
title_fullStr |
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
title_full_unstemmed |
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
title_sort |
expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2024 |
url |
https://doi.org/10.4103/apjtm.apjtm_876_23 https://doaj.org/article/52dbdd437d8d48dfb564a0aa9f0a3a53 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 17, Iss 4, Pp 173-183 (2024) |
op_relation |
https://journals.lww.com/10.4103/apjtm.apjtm_876_23 https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/apjtm.apjtm_876_23 https://doaj.org/article/52dbdd437d8d48dfb564a0aa9f0a3a53 |
op_doi |
https://doi.org/10.4103/apjtm.apjtm_876_23 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
17 |
container_issue |
4 |
container_start_page |
173 |
op_container_end_page |
183 |
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1809897248013680640 |