Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B
Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, Planococcus sp. ANT_H30 and...
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ftdoajarticles:oai:doaj.org/article:52cfe404ee444a9eb9a3f0e2d3cf3da3 2023-05-15T13:58:21+02:00 Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit 2020-09-01T00:00:00Z https://doi.org/10.3390/molecules25194357 https://doaj.org/article/52cfe404ee444a9eb9a3f0e2d3cf3da3 EN eng MDPI AG https://www.mdpi.com/1420-3049/25/19/4357 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25194357 1420-3049 https://doaj.org/article/52cfe404ee444a9eb9a3f0e2d3cf3da3 Molecules, Vol 25, Iss 4357, p 4357 (2020) antarctica carotenoid Planococcus Rhodococcus secondary metabolite Organic chemistry QD241-441 article 2020 ftdoajarticles https://doi.org/10.3390/molecules25194357 2022-12-31T13:35:41Z Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B. The physiological analysis of these strains revealed their potential to produce various biotechnologically valuable secondary metabolites, including surfactants, siderophores, and orange pigments. The genomic characterization of ANT_H30 and ANT_H53B allowed the identification of genes responsible for the production of carotenoids and the in silico reconstruction of the pigment biosynthesis pathways. The complex manual annotation of the bacterial genomes revealed the metabolic potential to degrade a wide variety of compounds, including xenobiotics and waste materials. Carotenoids produced by these bacteria were analyzed chromatographically, and we proved their activity as scavengers of free radicals. The quantity of crude carotenoid extracts produced at two temperatures using various media was also determined. This was a step toward the optimization of carotenoid production by Antarctic bacteria on a larger scale. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Molecules 25 19 4357 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctica carotenoid Planococcus Rhodococcus secondary metabolite Organic chemistry QD241-441 |
spellingShingle |
antarctica carotenoid Planococcus Rhodococcus secondary metabolite Organic chemistry QD241-441 Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
topic_facet |
antarctica carotenoid Planococcus Rhodococcus secondary metabolite Organic chemistry QD241-441 |
description |
Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B. The physiological analysis of these strains revealed their potential to produce various biotechnologically valuable secondary metabolites, including surfactants, siderophores, and orange pigments. The genomic characterization of ANT_H30 and ANT_H53B allowed the identification of genes responsible for the production of carotenoids and the in silico reconstruction of the pigment biosynthesis pathways. The complex manual annotation of the bacterial genomes revealed the metabolic potential to degrade a wide variety of compounds, including xenobiotics and waste materials. Carotenoids produced by these bacteria were analyzed chromatographically, and we proved their activity as scavengers of free radicals. The quantity of crude carotenoid extracts produced at two temperatures using various media was also determined. This was a step toward the optimization of carotenoid production by Antarctic bacteria on a larger scale. |
format |
Article in Journal/Newspaper |
author |
Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit |
author_facet |
Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit |
author_sort |
Michal Styczynski |
title |
Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
title_short |
Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
title_full |
Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
title_fullStr |
Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
title_full_unstemmed |
Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B |
title_sort |
genome-based insights into the production of carotenoids by antarctic bacteria, planococcus sp. ant_h30 and rhodococcus sp. ant_h53b |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25194357 https://doaj.org/article/52cfe404ee444a9eb9a3f0e2d3cf3da3 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Molecules, Vol 25, Iss 4357, p 4357 (2020) |
op_relation |
https://www.mdpi.com/1420-3049/25/19/4357 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25194357 1420-3049 https://doaj.org/article/52cfe404ee444a9eb9a3f0e2d3cf3da3 |
op_doi |
https://doi.org/10.3390/molecules25194357 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
19 |
container_start_page |
4357 |
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1766266580692369408 |