FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA
SUMMARY Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and Ja...
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ftdoajarticles:oai:doaj.org/article:52c64165d2984bc29d9a99769d96b948 2024-09-09T19:26:15+00:00 FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA Babatunde Olanrewaju MOTAYO Adekunle Johnson ADENIJI Adedayo Omotayo FANEYE https://doi.org/10.1590/S1678-9946201658074 https://doaj.org/article/52c64165d2984bc29d9a99769d96b948 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000256&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658074 https://doaj.org/article/52c64165d2984bc29d9a99769d96b948 Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 Rotavirus Sewage VP7 genotyping Nigeria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/S1678-9946201658074 2024-08-05T17:49:31Z SUMMARY Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 6000 and dextran was used to concentrate sewage samples following WHO protocols. Molecular detection was performed by RT-PCR, and VP7 genotyping by semi-nested multiplex PCR. A total of 14.2% (n = 27) samples tested positive. Monthly distribution showed that June to September had a lower rate (3.7% to 7.4%), while October to January recorded 11% to 26%. Genotype G1 predominated followed by G8, G9, G4 and lastly G2, 7.4% (n = 2) of isolates were nontypeable. This is the first report of rotavirus detection in sewages from Nigeria. Genotype G1 remains the most prevalent genotype. This observation calls for an effort by the governmental authorities to implement a molecular surveillance, both clinical and environmental, in order to provide vital information for the control and the vaccine efficacy not only in Nigeria, but globally. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 58 0 |
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English |
topic |
Rotavirus Sewage VP7 genotyping Nigeria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Rotavirus Sewage VP7 genotyping Nigeria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Babatunde Olanrewaju MOTAYO Adekunle Johnson ADENIJI Adedayo Omotayo FANEYE FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
topic_facet |
Rotavirus Sewage VP7 genotyping Nigeria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
SUMMARY Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 6000 and dextran was used to concentrate sewage samples following WHO protocols. Molecular detection was performed by RT-PCR, and VP7 genotyping by semi-nested multiplex PCR. A total of 14.2% (n = 27) samples tested positive. Monthly distribution showed that June to September had a lower rate (3.7% to 7.4%), while October to January recorded 11% to 26%. Genotype G1 predominated followed by G8, G9, G4 and lastly G2, 7.4% (n = 2) of isolates were nontypeable. This is the first report of rotavirus detection in sewages from Nigeria. Genotype G1 remains the most prevalent genotype. This observation calls for an effort by the governmental authorities to implement a molecular surveillance, both clinical and environmental, in order to provide vital information for the control and the vaccine efficacy not only in Nigeria, but globally. |
format |
Article in Journal/Newspaper |
author |
Babatunde Olanrewaju MOTAYO Adekunle Johnson ADENIJI Adedayo Omotayo FANEYE |
author_facet |
Babatunde Olanrewaju MOTAYO Adekunle Johnson ADENIJI Adedayo Omotayo FANEYE |
author_sort |
Babatunde Olanrewaju MOTAYO |
title |
FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
title_short |
FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
title_full |
FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
title_fullStr |
FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
title_full_unstemmed |
FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA |
title_sort |
first molecular detection and vp7 (g) genotyping of group a rotavirus by semi-nested rt-pcr from sewage in nigeria |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/S1678-9946201658074 https://doaj.org/article/52c64165d2984bc29d9a99769d96b948 |
geographic |
Arctic |
geographic_facet |
Arctic |
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Arctic |
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Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000256&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S1678-9946201658074 https://doaj.org/article/52c64165d2984bc29d9a99769d96b948 |
op_doi |
https://doi.org/10.1590/S1678-9946201658074 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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58 |
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