Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua
Abstract Background Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:52aadd58a2ff40aba4cbf183f724eb06 2023-05-15T15:06:58+02:00 Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua Ana Claudia Sato Rosemary Viola Bosch Sonia Elisabete Alves Will Miryam Paola Alvarez-Flores Mauricio Barbugiani Goldfeder Kerly Fernanda Mesquita Pasqualoto Bárbara Athayde Vaz Galvão da Silva Sonia Aparecida de Andrade Ana Marisa Chudzinski-Tavassi 2017-01-01T00:00:00Z https://doi.org/10.1186/s40409-016-0093-4 https://doaj.org/article/52aadd58a2ff40aba4cbf183f724eb06 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-016-0093-4 https://doaj.org/article/52aadd58a2ff40aba4cbf183f724eb06 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2017) Hemolin rLosac Wound healing Collagen Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2017 ftdoajarticles https://doi.org/10.1186/s40409-016-0093-4 2022-12-31T04:27:17Z Abstract Background Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats. Methods Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses. Results The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate. Conclusion The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 22 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Hemolin rLosac Wound healing Collagen Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Hemolin rLosac Wound healing Collagen Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Ana Claudia Sato Rosemary Viola Bosch Sonia Elisabete Alves Will Miryam Paola Alvarez-Flores Mauricio Barbugiani Goldfeder Kerly Fernanda Mesquita Pasqualoto Bárbara Athayde Vaz Galvão da Silva Sonia Aparecida de Andrade Ana Marisa Chudzinski-Tavassi Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
topic_facet |
Hemolin rLosac Wound healing Collagen Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats. Methods Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses. Results The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate. Conclusion The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations. |
format |
Article in Journal/Newspaper |
author |
Ana Claudia Sato Rosemary Viola Bosch Sonia Elisabete Alves Will Miryam Paola Alvarez-Flores Mauricio Barbugiani Goldfeder Kerly Fernanda Mesquita Pasqualoto Bárbara Athayde Vaz Galvão da Silva Sonia Aparecida de Andrade Ana Marisa Chudzinski-Tavassi |
author_facet |
Ana Claudia Sato Rosemary Viola Bosch Sonia Elisabete Alves Will Miryam Paola Alvarez-Flores Mauricio Barbugiani Goldfeder Kerly Fernanda Mesquita Pasqualoto Bárbara Athayde Vaz Galvão da Silva Sonia Aparecida de Andrade Ana Marisa Chudzinski-Tavassi |
author_sort |
Ana Claudia Sato |
title |
Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
title_short |
Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
title_full |
Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
title_fullStr |
Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
title_full_unstemmed |
Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua |
title_sort |
exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar lonomia obliqua |
publisher |
SciELO |
publishDate |
2017 |
url |
https://doi.org/10.1186/s40409-016-0093-4 https://doaj.org/article/52aadd58a2ff40aba4cbf183f724eb06 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2017) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-016-0093-4 https://doaj.org/article/52aadd58a2ff40aba4cbf183f724eb06 |
op_doi |
https://doi.org/10.1186/s40409-016-0093-4 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
22 |
container_issue |
1 |
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1766338553820741632 |