Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment
Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers....
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Online Access: | https://doi.org/10.3390/microorganisms10081673 https://doaj.org/article/5263eab099f2476cb08d6a481cbe8e63 |
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ftdoajarticles:oai:doaj.org/article:5263eab099f2476cb08d6a481cbe8e63 2023-05-15T13:57:29+02:00 Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado 2022-08-01T00:00:00Z https://doi.org/10.3390/microorganisms10081673 https://doaj.org/article/5263eab099f2476cb08d6a481cbe8e63 EN eng MDPI AG https://www.mdpi.com/2076-2607/10/8/1673 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10081673 2076-2607 https://doaj.org/article/5263eab099f2476cb08d6a481cbe8e63 Microorganisms, Vol 10, Iss 1673, p 1673 (2022) Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/microorganisms10081673 2022-12-31T01:10:08Z Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers. From the sediments of Deception Island, we isolated representatives of the genus Anoxybacillus , a widely spread genus that is mainly encountered in thermophilic environments. However, the phylogeny of this genus and its adaptive mechanisms in the geothermal sites of cold environments remain unknown. To the best of our knowledge, this is the first study to unravel the genomic features and provide insights into the phylogenomics and metabolic potential of members of the genus Anoxybacillus inhabiting the Antarctic thermophilic ecosystem. Here, we report the genome sequencing data of seven A. flavithermus strains isolated from two geothermal sites on Deception Island, Antarctic Peninsula. Their genomes were approximately 3.0 Mb in size, had a G + C ratio of 42%, and were predicted to encode 3500 proteins on average. We observed that the strains were phylogenomically closest to each other (Average Nucleotide Identity (ANI) > 98%) and to A. flavithermus (ANI 95%). In silico genomic analysis revealed 15 resistance and metabolic islands, as well as genes related to genome stabilization, DNA repair systems against UV radiation threats, temperature adaptation, heat- and cold-shock proteins (Csps), and resistance to alkaline conditions. Remarkably, glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences were predicted, revealing metabolic and cellular capabilities for potential biotechnological applications. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Antarctic Peninsula Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Microorganisms 10 8 1673 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Biology (General) QH301-705.5 |
spellingShingle |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Biology (General) QH301-705.5 Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
topic_facet |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Biology (General) QH301-705.5 |
description |
Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers. From the sediments of Deception Island, we isolated representatives of the genus Anoxybacillus , a widely spread genus that is mainly encountered in thermophilic environments. However, the phylogeny of this genus and its adaptive mechanisms in the geothermal sites of cold environments remain unknown. To the best of our knowledge, this is the first study to unravel the genomic features and provide insights into the phylogenomics and metabolic potential of members of the genus Anoxybacillus inhabiting the Antarctic thermophilic ecosystem. Here, we report the genome sequencing data of seven A. flavithermus strains isolated from two geothermal sites on Deception Island, Antarctic Peninsula. Their genomes were approximately 3.0 Mb in size, had a G + C ratio of 42%, and were predicted to encode 3500 proteins on average. We observed that the strains were phylogenomically closest to each other (Average Nucleotide Identity (ANI) > 98%) and to A. flavithermus (ANI 95%). In silico genomic analysis revealed 15 resistance and metabolic islands, as well as genes related to genome stabilization, DNA repair systems against UV radiation threats, temperature adaptation, heat- and cold-shock proteins (Csps), and resistance to alkaline conditions. Remarkably, glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences were predicted, revealing metabolic and cellular capabilities for potential biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado |
author_facet |
Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado |
author_sort |
Júnia Schultz |
title |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_short |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_full |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_fullStr |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_full_unstemmed |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_sort |
unraveling the genomic potential of the thermophilic bacterium anoxybacillus flavithermus from an antarctic geothermal environment |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10081673 https://doaj.org/article/5263eab099f2476cb08d6a481cbe8e63 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic The Antarctic Antarctic Peninsula Deception Island |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula Deception Island |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island |
op_source |
Microorganisms, Vol 10, Iss 1673, p 1673 (2022) |
op_relation |
https://www.mdpi.com/2076-2607/10/8/1673 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10081673 2076-2607 https://doaj.org/article/5263eab099f2476cb08d6a481cbe8e63 |
op_doi |
https://doi.org/10.3390/microorganisms10081673 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
8 |
container_start_page |
1673 |
_version_ |
1766265158533906432 |