Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae
Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. cor...
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ftdoajarticles:oai:doaj.org/article:525abfd3ad1b48e2b598add3606067d7 2023-05-15T15:58:22+02:00 Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae Hyoun Joong Kim Jin Woo Jun Sib Sankar Giri Cheng Chi Saekil Yun Sang Guen Kim Sang Wha Kim Se Jin Han Jun Kwon Woo Taek Oh Sung Bin Lee Ji Hyung Kim Se Chang Park 2020-03-01T00:00:00Z https://doi.org/10.3390/pathogens9030206 https://doaj.org/article/525abfd3ad1b48e2b598add3606067d7 EN eng MDPI AG https://www.mdpi.com/2076-0817/9/3/206 https://doaj.org/toc/2076-0817 2076-0817 doi:10.3390/pathogens9030206 https://doaj.org/article/525abfd3ad1b48e2b598add3606067d7 Pathogens, Vol 9, Iss 3, p 206 (2020) vibrio species pacific oyster ( crassostrea gigas ) larvae genome analyses chri chrii snuty-1 Medicine R article 2020 ftdoajarticles https://doi.org/10.3390/pathogens9030206 2022-12-30T19:57:02Z Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 10 5 CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Pathogens 9 3 206 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
vibrio species pacific oyster ( crassostrea gigas ) larvae genome analyses chri chrii snuty-1 Medicine R |
spellingShingle |
vibrio species pacific oyster ( crassostrea gigas ) larvae genome analyses chri chrii snuty-1 Medicine R Hyoun Joong Kim Jin Woo Jun Sib Sankar Giri Cheng Chi Saekil Yun Sang Guen Kim Sang Wha Kim Se Jin Han Jun Kwon Woo Taek Oh Sung Bin Lee Ji Hyung Kim Se Chang Park Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
topic_facet |
vibrio species pacific oyster ( crassostrea gigas ) larvae genome analyses chri chrii snuty-1 Medicine R |
description |
Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 10 5 CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors. |
format |
Article in Journal/Newspaper |
author |
Hyoun Joong Kim Jin Woo Jun Sib Sankar Giri Cheng Chi Saekil Yun Sang Guen Kim Sang Wha Kim Se Jin Han Jun Kwon Woo Taek Oh Sung Bin Lee Ji Hyung Kim Se Chang Park |
author_facet |
Hyoun Joong Kim Jin Woo Jun Sib Sankar Giri Cheng Chi Saekil Yun Sang Guen Kim Sang Wha Kim Se Jin Han Jun Kwon Woo Taek Oh Sung Bin Lee Ji Hyung Kim Se Chang Park |
author_sort |
Hyoun Joong Kim |
title |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
title_short |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
title_full |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
title_fullStr |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
title_full_unstemmed |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster ( Crassostrea gigas ) Larvae |
title_sort |
identification and genome analysis of vibrio coralliilyticus causing mortality of pacific oyster ( crassostrea gigas ) larvae |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9030206 https://doaj.org/article/525abfd3ad1b48e2b598add3606067d7 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Pathogens, Vol 9, Iss 3, p 206 (2020) |
op_relation |
https://www.mdpi.com/2076-0817/9/3/206 https://doaj.org/toc/2076-0817 2076-0817 doi:10.3390/pathogens9030206 https://doaj.org/article/525abfd3ad1b48e2b598add3606067d7 |
op_doi |
https://doi.org/10.3390/pathogens9030206 |
container_title |
Pathogens |
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9 |
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3 |
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206 |
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1766394105981566976 |