Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi
Fucosylated chondroitin sulfate CD was isolated from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf of the eastern coast of Kamchatka. Structural characterization of CD was performed using a series of non-destructive NMR spectroscopic procedures. The polysaccharide was shown...
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ftdoajarticles:oai:doaj.org/article:52331ca0b8b84df2a36f54993dbc039b 2023-05-15T16:59:17+02:00 Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi Nadezhda E. Ustyuzhanina Maria I. Bilan Elena G. Panina Nadezhda P. Sanamyan Andrey S. Dmitrenok Eugenia A. Tsvetkova Natalia A. Ushakova Alexander S. Shashkov Nikolay E. Nifantiev Anatolii I. Usov 2018-10-01T00:00:00Z https://doi.org/10.3390/md16100389 https://doaj.org/article/52331ca0b8b84df2a36f54993dbc039b EN eng MDPI AG http://www.mdpi.com/1660-3397/16/10/389 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100389 https://doaj.org/article/52331ca0b8b84df2a36f54993dbc039b Marine Drugs, Vol 16, Iss 10, p 389 (2018) fucosylated chondroitin sulfate Cucumaria djakonovi sea cucumber structure anti-inflammatory activity Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.3390/md16100389 2022-12-30T20:46:05Z Fucosylated chondroitin sulfate CD was isolated from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf of the eastern coast of Kamchatka. Structural characterization of CD was performed using a series of non-destructive NMR spectroscopic procedures. The polysaccharide was shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n where about 60% of GlcA residues were 3-O-fucosylated, while another part of GlcA units did not contain any substituents. The presence of unsubstituted both at O-2 and O-3 glucuronic acid residues in a structure of holothurian chondroitin sulfate is unusual and has not been reported previously. Three different fucosyl branches Fucp2S4S, Fucp3S4S and Fucp4S were found in the ratio of 2:1:1. The GalNAc units were mono- or disulfated at positions 4 and 6. Anti-inflammatory activity of CD was assessed on a model of acute peritoneal inflammation in rats. About 45% inhibition was found for CD, while a structurally related linear chondroitin sulfate SS from cartilage of the fish Salmo salar demonstrated only 31% inhibition, indicating that the presence of sulfated fucosyl branches is essential for anti-inflammatory effect of chondroitin sulfates of marine origin. Article in Journal/Newspaper Kamchatka Salmo salar Directory of Open Access Journals: DOAJ Articles Marine Drugs 16 10 389 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fucosylated chondroitin sulfate Cucumaria djakonovi sea cucumber structure anti-inflammatory activity Biology (General) QH301-705.5 |
spellingShingle |
fucosylated chondroitin sulfate Cucumaria djakonovi sea cucumber structure anti-inflammatory activity Biology (General) QH301-705.5 Nadezhda E. Ustyuzhanina Maria I. Bilan Elena G. Panina Nadezhda P. Sanamyan Andrey S. Dmitrenok Eugenia A. Tsvetkova Natalia A. Ushakova Alexander S. Shashkov Nikolay E. Nifantiev Anatolii I. Usov Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
topic_facet |
fucosylated chondroitin sulfate Cucumaria djakonovi sea cucumber structure anti-inflammatory activity Biology (General) QH301-705.5 |
description |
Fucosylated chondroitin sulfate CD was isolated from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf of the eastern coast of Kamchatka. Structural characterization of CD was performed using a series of non-destructive NMR spectroscopic procedures. The polysaccharide was shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n where about 60% of GlcA residues were 3-O-fucosylated, while another part of GlcA units did not contain any substituents. The presence of unsubstituted both at O-2 and O-3 glucuronic acid residues in a structure of holothurian chondroitin sulfate is unusual and has not been reported previously. Three different fucosyl branches Fucp2S4S, Fucp3S4S and Fucp4S were found in the ratio of 2:1:1. The GalNAc units were mono- or disulfated at positions 4 and 6. Anti-inflammatory activity of CD was assessed on a model of acute peritoneal inflammation in rats. About 45% inhibition was found for CD, while a structurally related linear chondroitin sulfate SS from cartilage of the fish Salmo salar demonstrated only 31% inhibition, indicating that the presence of sulfated fucosyl branches is essential for anti-inflammatory effect of chondroitin sulfates of marine origin. |
format |
Article in Journal/Newspaper |
author |
Nadezhda E. Ustyuzhanina Maria I. Bilan Elena G. Panina Nadezhda P. Sanamyan Andrey S. Dmitrenok Eugenia A. Tsvetkova Natalia A. Ushakova Alexander S. Shashkov Nikolay E. Nifantiev Anatolii I. Usov |
author_facet |
Nadezhda E. Ustyuzhanina Maria I. Bilan Elena G. Panina Nadezhda P. Sanamyan Andrey S. Dmitrenok Eugenia A. Tsvetkova Natalia A. Ushakova Alexander S. Shashkov Nikolay E. Nifantiev Anatolii I. Usov |
author_sort |
Nadezhda E. Ustyuzhanina |
title |
Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
title_short |
Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
title_full |
Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
title_fullStr |
Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
title_full_unstemmed |
Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi |
title_sort |
structure and anti-inflammatory activity of a new unusual fucosylated chondroitin sulfate from cucumaria djakonovi |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16100389 https://doaj.org/article/52331ca0b8b84df2a36f54993dbc039b |
genre |
Kamchatka Salmo salar |
genre_facet |
Kamchatka Salmo salar |
op_source |
Marine Drugs, Vol 16, Iss 10, p 389 (2018) |
op_relation |
http://www.mdpi.com/1660-3397/16/10/389 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100389 https://doaj.org/article/52331ca0b8b84df2a36f54993dbc039b |
op_doi |
https://doi.org/10.3390/md16100389 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
10 |
container_start_page |
389 |
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1766051515454193664 |