Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.

Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be...

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Published in:PLoS Neglected Tropical Diseases
Main Authors: Ana Paula Souza, Bruno Bezerril Andrade, Dorlene Aquino, Petter Entringer, José Carlos Miranda, Ruan Alcantara, Daniel Ruiz, Manuel Soto, Clarissa R Teixeira, Jesus G Valenzuela, Camila Indiani de Oliveira, Cláudia Ida Brodskyn, Manoel Barral-Netto, Aldina Barral
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2010
Subjects:
Online Access:https://doi.org/10.1371/journal.pntd.0000649
https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776
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spelling ftdoajarticles:oai:doaj.org/article:52283fe481ef4f05999c62dd4ad81776 2023-05-15T15:16:25+02:00 Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R Teixeira Jesus G Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral 2010-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2843636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e649 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000649 2022-12-31T13:30:44Z Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be used as a marker of exposure to the vector. Performing large-scale serological studies to detect vector exposure has been limited by the difficulty in obtaining sand fly saliva. Here, we validate the use of two sand fly salivary recombinant proteins as markers for vector exposure.ELISA was used to screen human sera, collected in an area endemic for visceral leishmaniasis, against the salivary gland sonicate (SGS) or two recombinant proteins (rLJM11 and rLJM17) from Lutzomyia longipalpis saliva. Antibody levels before and after SGS seroconversion (n = 26) were compared using the Wilcoxon signed rank paired test. Human sera from an area endemic for VL which recognize Lu. longipalpis saliva in ELISA also recognize a combination of rLJM17 and rLJM11. We then extended the analysis to include 40 sera from individuals who were seropositive and 40 seronegative to Lu. longipalpis SGS. Each recombinant protein was able to detect anti-saliva seroconversion, whereas the two proteins combined increased the detection significantly. Additionally, we evaluated the specificity of the anti-Lu. longipalpis response by testing 40 sera positive to Lutzomyia intermedia SGS, and very limited (2/40) cross-reactivity was observed. Receiver-operator characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for the prediction of anti-SGS positivity. These ROC curves evidenced the superior performance of rLJM17+rLJM11. Predicted threshold levels were confirmed for rLJM17+rLJM11 using a large panel of 1,077 serum samples.Our results show the possibility of substituting Lu. longipalpis SGS for two recombinant proteins, LJM17 and LJM11, in order to probe for vector exposure in individuals residing in endemic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 3 e649
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Ana Paula Souza
Bruno Bezerril Andrade
Dorlene Aquino
Petter Entringer
José Carlos Miranda
Ruan Alcantara
Daniel Ruiz
Manuel Soto
Clarissa R Teixeira
Jesus G Valenzuela
Camila Indiani de Oliveira
Cláudia Ida Brodskyn
Manoel Barral-Netto
Aldina Barral
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
topic_facet Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be used as a marker of exposure to the vector. Performing large-scale serological studies to detect vector exposure has been limited by the difficulty in obtaining sand fly saliva. Here, we validate the use of two sand fly salivary recombinant proteins as markers for vector exposure.ELISA was used to screen human sera, collected in an area endemic for visceral leishmaniasis, against the salivary gland sonicate (SGS) or two recombinant proteins (rLJM11 and rLJM17) from Lutzomyia longipalpis saliva. Antibody levels before and after SGS seroconversion (n = 26) were compared using the Wilcoxon signed rank paired test. Human sera from an area endemic for VL which recognize Lu. longipalpis saliva in ELISA also recognize a combination of rLJM17 and rLJM11. We then extended the analysis to include 40 sera from individuals who were seropositive and 40 seronegative to Lu. longipalpis SGS. Each recombinant protein was able to detect anti-saliva seroconversion, whereas the two proteins combined increased the detection significantly. Additionally, we evaluated the specificity of the anti-Lu. longipalpis response by testing 40 sera positive to Lutzomyia intermedia SGS, and very limited (2/40) cross-reactivity was observed. Receiver-operator characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for the prediction of anti-SGS positivity. These ROC curves evidenced the superior performance of rLJM17+rLJM11. Predicted threshold levels were confirmed for rLJM17+rLJM11 using a large panel of 1,077 serum samples.Our results show the possibility of substituting Lu. longipalpis SGS for two recombinant proteins, LJM17 and LJM11, in order to probe for vector exposure in individuals residing in endemic ...
format Article in Journal/Newspaper
author Ana Paula Souza
Bruno Bezerril Andrade
Dorlene Aquino
Petter Entringer
José Carlos Miranda
Ruan Alcantara
Daniel Ruiz
Manuel Soto
Clarissa R Teixeira
Jesus G Valenzuela
Camila Indiani de Oliveira
Cláudia Ida Brodskyn
Manoel Barral-Netto
Aldina Barral
author_facet Ana Paula Souza
Bruno Bezerril Andrade
Dorlene Aquino
Petter Entringer
José Carlos Miranda
Ruan Alcantara
Daniel Ruiz
Manuel Soto
Clarissa R Teixeira
Jesus G Valenzuela
Camila Indiani de Oliveira
Cláudia Ida Brodskyn
Manoel Barral-Netto
Aldina Barral
author_sort Ana Paula Souza
title Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
title_short Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
title_full Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
title_fullStr Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
title_full_unstemmed Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
title_sort using recombinant proteins from lutzomyia longipalpis saliva to estimate human vector exposure in visceral leishmaniasis endemic areas.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doi.org/10.1371/journal.pntd.0000649
https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e649 (2010)
op_relation http://europepmc.org/articles/PMC2843636?pdf=render
https://doaj.org/toc/1935-2727
https://doaj.org/toc/1935-2735
1935-2727
1935-2735
doi:10.1371/journal.pntd.0000649
https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776
op_doi https://doi.org/10.1371/journal.pntd.0000649
container_title PLoS Neglected Tropical Diseases
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container_issue 3
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