Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas.
Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be...
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2010
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ftdoajarticles:oai:doaj.org/article:52283fe481ef4f05999c62dd4ad81776 2023-05-15T15:16:25+02:00 Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R Teixeira Jesus G Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral 2010-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2843636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e649 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000649 2022-12-31T13:30:44Z Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be used as a marker of exposure to the vector. Performing large-scale serological studies to detect vector exposure has been limited by the difficulty in obtaining sand fly saliva. Here, we validate the use of two sand fly salivary recombinant proteins as markers for vector exposure.ELISA was used to screen human sera, collected in an area endemic for visceral leishmaniasis, against the salivary gland sonicate (SGS) or two recombinant proteins (rLJM11 and rLJM17) from Lutzomyia longipalpis saliva. Antibody levels before and after SGS seroconversion (n = 26) were compared using the Wilcoxon signed rank paired test. Human sera from an area endemic for VL which recognize Lu. longipalpis saliva in ELISA also recognize a combination of rLJM17 and rLJM11. We then extended the analysis to include 40 sera from individuals who were seropositive and 40 seronegative to Lu. longipalpis SGS. Each recombinant protein was able to detect anti-saliva seroconversion, whereas the two proteins combined increased the detection significantly. Additionally, we evaluated the specificity of the anti-Lu. longipalpis response by testing 40 sera positive to Lutzomyia intermedia SGS, and very limited (2/40) cross-reactivity was observed. Receiver-operator characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for the prediction of anti-SGS positivity. These ROC curves evidenced the superior performance of rLJM17+rLJM11. Predicted threshold levels were confirmed for rLJM17+rLJM11 using a large panel of 1,077 serum samples.Our results show the possibility of substituting Lu. longipalpis SGS for two recombinant proteins, LJM17 and LJM11, in order to probe for vector exposure in individuals residing in endemic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 3 e649 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R Teixeira Jesus G Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. The exposure to vector saliva induces an immune response against its components that can be used as a marker of exposure to the vector. Performing large-scale serological studies to detect vector exposure has been limited by the difficulty in obtaining sand fly saliva. Here, we validate the use of two sand fly salivary recombinant proteins as markers for vector exposure.ELISA was used to screen human sera, collected in an area endemic for visceral leishmaniasis, against the salivary gland sonicate (SGS) or two recombinant proteins (rLJM11 and rLJM17) from Lutzomyia longipalpis saliva. Antibody levels before and after SGS seroconversion (n = 26) were compared using the Wilcoxon signed rank paired test. Human sera from an area endemic for VL which recognize Lu. longipalpis saliva in ELISA also recognize a combination of rLJM17 and rLJM11. We then extended the analysis to include 40 sera from individuals who were seropositive and 40 seronegative to Lu. longipalpis SGS. Each recombinant protein was able to detect anti-saliva seroconversion, whereas the two proteins combined increased the detection significantly. Additionally, we evaluated the specificity of the anti-Lu. longipalpis response by testing 40 sera positive to Lutzomyia intermedia SGS, and very limited (2/40) cross-reactivity was observed. Receiver-operator characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for the prediction of anti-SGS positivity. These ROC curves evidenced the superior performance of rLJM17+rLJM11. Predicted threshold levels were confirmed for rLJM17+rLJM11 using a large panel of 1,077 serum samples.Our results show the possibility of substituting Lu. longipalpis SGS for two recombinant proteins, LJM17 and LJM11, in order to probe for vector exposure in individuals residing in endemic ... |
format |
Article in Journal/Newspaper |
author |
Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R Teixeira Jesus G Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral |
author_facet |
Ana Paula Souza Bruno Bezerril Andrade Dorlene Aquino Petter Entringer José Carlos Miranda Ruan Alcantara Daniel Ruiz Manuel Soto Clarissa R Teixeira Jesus G Valenzuela Camila Indiani de Oliveira Cláudia Ida Brodskyn Manoel Barral-Netto Aldina Barral |
author_sort |
Ana Paula Souza |
title |
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
title_short |
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
title_full |
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
title_fullStr |
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
title_full_unstemmed |
Using recombinant proteins from Lutzomyia longipalpis saliva to estimate human vector exposure in visceral Leishmaniasis endemic areas. |
title_sort |
using recombinant proteins from lutzomyia longipalpis saliva to estimate human vector exposure in visceral leishmaniasis endemic areas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e649 (2010) |
op_relation |
http://europepmc.org/articles/PMC2843636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000649 https://doaj.org/article/52283fe481ef4f05999c62dd4ad81776 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000649 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
3 |
container_start_page |
e649 |
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1766346705993728000 |