Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major
Objective: To evaluate leishmanicidal effects of Euphorbia erythadenia plant extract. Methods: Extraction was done using methanolic Soxhlet of dried and ground aerial parts of the plant. Then, five different extract concentrations, in addition of positive, negative and solvent controls were prepared...
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Wolters Kluwer Medknow Publications
2014
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Online Access: | https://doi.org/10.12980/APJTB.4.2014C1018 https://doaj.org/article/5210caf017c94d9da95445e9dcbbfa9c |
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ftdoajarticles:oai:doaj.org/article:5210caf017c94d9da95445e9dcbbfa9c 2023-05-15T15:06:58+02:00 Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major Reza Kazemi Oskuee Mahmoud Reza Jaafari Sara Amani Mohammad Ramezani 2014-07-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1018 https://doaj.org/article/5210caf017c94d9da95445e9dcbbfa9c EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300526 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1018 https://doaj.org/article/5210caf017c94d9da95445e9dcbbfa9c Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S581-S583 (2014) Euphorbia erythadenia Leishmanicidal activity Leishmania major Promastigotes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1018 2022-12-31T09:50:56Z Objective: To evaluate leishmanicidal effects of Euphorbia erythadenia plant extract. Methods: Extraction was done using methanolic Soxhlet of dried and ground aerial parts of the plant. Then, five different extract concentrations, in addition of positive, negative and solvent controls were prepared and added to a 24-well plate containing 40 000 parasites/well. The extract concentrations were 1, 0.5, 0.25, 0.125 and 0.062 5 mg/mL. Amphotricin B (0.5 mg/mL) was used as positive control while negative control contained only culture medium. After 3 d incubation at 25 °C the amount of parasites in each well was determined on each day of experiment microscopially using Neubar chamber. Results: Soxhlet extract as well as amphotricin B killed all parasites at concentration of 1 mg/mL. The leshmanicidal activity of lower doses of extract was dose-dependent. The EC50 for Soxhlet extracts in dimethylsulfoxide was 0.30 mg/mL. The EC50 for Soxhlet extracts in methanol was 0.23 mg/mL. No obvious effects from the control solvent on the Leishmania major promastigotes were observed. Conclusions: The Soxhlet extract of Euphorbia erythadenia showed suitable leishmanicidal activity, especially in higher concentration fractions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S581 S583 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Euphorbia erythadenia Leishmanicidal activity Leishmania major Promastigotes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Euphorbia erythadenia Leishmanicidal activity Leishmania major Promastigotes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Reza Kazemi Oskuee Mahmoud Reza Jaafari Sara Amani Mohammad Ramezani Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
topic_facet |
Euphorbia erythadenia Leishmanicidal activity Leishmania major Promastigotes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate leishmanicidal effects of Euphorbia erythadenia plant extract. Methods: Extraction was done using methanolic Soxhlet of dried and ground aerial parts of the plant. Then, five different extract concentrations, in addition of positive, negative and solvent controls were prepared and added to a 24-well plate containing 40 000 parasites/well. The extract concentrations were 1, 0.5, 0.25, 0.125 and 0.062 5 mg/mL. Amphotricin B (0.5 mg/mL) was used as positive control while negative control contained only culture medium. After 3 d incubation at 25 °C the amount of parasites in each well was determined on each day of experiment microscopially using Neubar chamber. Results: Soxhlet extract as well as amphotricin B killed all parasites at concentration of 1 mg/mL. The leshmanicidal activity of lower doses of extract was dose-dependent. The EC50 for Soxhlet extracts in dimethylsulfoxide was 0.30 mg/mL. The EC50 for Soxhlet extracts in methanol was 0.23 mg/mL. No obvious effects from the control solvent on the Leishmania major promastigotes were observed. Conclusions: The Soxhlet extract of Euphorbia erythadenia showed suitable leishmanicidal activity, especially in higher concentration fractions. |
format |
Article in Journal/Newspaper |
author |
Reza Kazemi Oskuee Mahmoud Reza Jaafari Sara Amani Mohammad Ramezani |
author_facet |
Reza Kazemi Oskuee Mahmoud Reza Jaafari Sara Amani Mohammad Ramezani |
author_sort |
Reza Kazemi Oskuee |
title |
Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
title_short |
Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
title_full |
Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
title_fullStr |
Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
title_full_unstemmed |
Evaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major |
title_sort |
evaluation of leishmanicidal effect of euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of leishmania major |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1018 https://doaj.org/article/5210caf017c94d9da95445e9dcbbfa9c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S581-S583 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300526 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1018 https://doaj.org/article/5210caf017c94d9da95445e9dcbbfa9c |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1018 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S581 |
op_container_end_page |
S583 |
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1766338552330715136 |