Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus.
Background Chikungunya virus (CHIKV) reemerged and caused millions of human infections since 2004. The disease could be established, when the virus has been introduced to areas where the appropriate vectors are endemic. The differential diagnosis of CHIKV infection varies based on place of residence...
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ftdoajarticles:oai:doaj.org/article:5208706ce5a54afe8736145935428b3e 2023-05-15T15:18:10+02:00 Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. Meijun Guo Shanshan Du Lijin Lai Wei Wu Xiaoxia Huang Aqian Li Hao Li Chuan Li Qin Wang Lina Sun Tiezhu Liu Tingting Tian Shiwen Wang Mifang Liang Dexin Li Chun Xie Jiandong Li 2022-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010829 https://doaj.org/article/5208706ce5a54afe8736145935428b3e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010829 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010829 https://doaj.org/article/5208706ce5a54afe8736145935428b3e PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0010829 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010829 2022-12-30T19:26:58Z Background Chikungunya virus (CHIKV) reemerged and caused millions of human infections since 2004. The disease could be established, when the virus has been introduced to areas where the appropriate vectors are endemic. The differential diagnosis of CHIKV infection varies based on place of residence, travel history, and exposures. Serological tests are commonly used to diagnose CHIKV infection, but their availability and assessments of the performance of the diagnostics have been limited. Objectives To develop and evaluate antibodies detection methods for chikungunya diagnosis and serological investigation. Methods Recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (Mac-ELISA) and double antigen sandwich ELISA (Das-ELISA) for detection of antibodies to Chikungunya virus were developed and evaluated. The repeatability was evaluated by testing of three reference sera at single dilutions in triplicated for 5 times. The sensitivity, specificity, accuracy, and agreement of the MAC-ELISA and Das-ELISA were obtained by comparing the detection results of 225 serum samples (45 positive; 180 negative) with a real-time RT-PCR assay and an IFA commercial tests manufactured by Euroimmun. Results The established ELISA assays were standardized by determining the optimal concentrations of the key reagents. The coefficient values of repeat testing were within 10% and 20% for intraassay and interassay precision, respectively. A sensitivity of 60.0% and 52.5%, a specificity of 96.2% and 96.8%, and an accuracy of 89.8% and 88.9% were obtained for the Mac-ELISA and Das-ELISA, respectively, when compared to a CHIKV qRT-PCR method. And a sensitivity of 100%, a specificity of 97.5% and 99.5%, and an accuracy of 97.8% and 99.6% were yielded respectively when using the IIFT as a reference method, which showed a highly consistence to the commercial IIFT assay with a Kappa value greater than 0.90. Conclusions The Mac-ELISA and Das-ELISA based on recombinant E2 protein of CHIKV were developed and standardized, which ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 12 e0010829 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Meijun Guo Shanshan Du Lijin Lai Wei Wu Xiaoxia Huang Aqian Li Hao Li Chuan Li Qin Wang Lina Sun Tiezhu Liu Tingting Tian Shiwen Wang Mifang Liang Dexin Li Chun Xie Jiandong Li Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Chikungunya virus (CHIKV) reemerged and caused millions of human infections since 2004. The disease could be established, when the virus has been introduced to areas where the appropriate vectors are endemic. The differential diagnosis of CHIKV infection varies based on place of residence, travel history, and exposures. Serological tests are commonly used to diagnose CHIKV infection, but their availability and assessments of the performance of the diagnostics have been limited. Objectives To develop and evaluate antibodies detection methods for chikungunya diagnosis and serological investigation. Methods Recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (Mac-ELISA) and double antigen sandwich ELISA (Das-ELISA) for detection of antibodies to Chikungunya virus were developed and evaluated. The repeatability was evaluated by testing of three reference sera at single dilutions in triplicated for 5 times. The sensitivity, specificity, accuracy, and agreement of the MAC-ELISA and Das-ELISA were obtained by comparing the detection results of 225 serum samples (45 positive; 180 negative) with a real-time RT-PCR assay and an IFA commercial tests manufactured by Euroimmun. Results The established ELISA assays were standardized by determining the optimal concentrations of the key reagents. The coefficient values of repeat testing were within 10% and 20% for intraassay and interassay precision, respectively. A sensitivity of 60.0% and 52.5%, a specificity of 96.2% and 96.8%, and an accuracy of 89.8% and 88.9% were obtained for the Mac-ELISA and Das-ELISA, respectively, when compared to a CHIKV qRT-PCR method. And a sensitivity of 100%, a specificity of 97.5% and 99.5%, and an accuracy of 97.8% and 99.6% were yielded respectively when using the IIFT as a reference method, which showed a highly consistence to the commercial IIFT assay with a Kappa value greater than 0.90. Conclusions The Mac-ELISA and Das-ELISA based on recombinant E2 protein of CHIKV were developed and standardized, which ... |
format |
Article in Journal/Newspaper |
author |
Meijun Guo Shanshan Du Lijin Lai Wei Wu Xiaoxia Huang Aqian Li Hao Li Chuan Li Qin Wang Lina Sun Tiezhu Liu Tingting Tian Shiwen Wang Mifang Liang Dexin Li Chun Xie Jiandong Li |
author_facet |
Meijun Guo Shanshan Du Lijin Lai Wei Wu Xiaoxia Huang Aqian Li Hao Li Chuan Li Qin Wang Lina Sun Tiezhu Liu Tingting Tian Shiwen Wang Mifang Liang Dexin Li Chun Xie Jiandong Li |
author_sort |
Meijun Guo |
title |
Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
title_short |
Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
title_full |
Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
title_fullStr |
Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
title_full_unstemmed |
Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. |
title_sort |
development and evaluation of recombinant e2 protein based igm capture enzyme-linked immunosorbent assay (elisa) and double antigen sandwich elisa for detection of antibodies to chikungunya virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010829 https://doaj.org/article/5208706ce5a54afe8736145935428b3e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0010829 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010829 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010829 https://doaj.org/article/5208706ce5a54afe8736145935428b3e |
op_doi |
https://doi.org/10.1371/journal.pntd.0010829 |
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PLOS Neglected Tropical Diseases |
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12 |
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e0010829 |
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