Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice
Acute lung injury (ALI) represents a life-threatening condition with high morbidity and mortality despite modern mechanical ventilators and multiple pharmacological strategies. Therefore, there is a need to develop efficacious interventions with minimal side effects. The anti-inflammatory activities...
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MDPI AG
2024
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ftdoajarticles:oai:doaj.org/article:51a7718b60cc418c9970ddd306e0ba3c 2024-09-15T18:03:24+00:00 Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice Oladapo F. Fagbohun Wasitha P. D. W. Thilakarathna Juan Zhou Christian Lehmann Guangling Jiao H. P. Vasantha Rupasinghe 2024-03-01T00:00:00Z https://doi.org/10.3390/molecules29071511 https://doaj.org/article/51a7718b60cc418c9970ddd306e0ba3c EN eng MDPI AG https://www.mdpi.com/1420-3049/29/7/1511 https://doaj.org/toc/1420-3049 doi:10.3390/molecules29071511 1420-3049 https://doaj.org/article/51a7718b60cc418c9970ddd306e0ba3c Molecules, Vol 29, Iss 7, p 1511 (2024) Cucumaria frondosa Vaccinium angustifolium ALI cytokine inflammation NF-κB Organic chemistry QD241-441 article 2024 ftdoajarticles https://doi.org/10.3390/molecules29071511 2024-08-05T17:49:37Z Acute lung injury (ALI) represents a life-threatening condition with high morbidity and mortality despite modern mechanical ventilators and multiple pharmacological strategies. Therefore, there is a need to develop efficacious interventions with minimal side effects. The anti-inflammatory activities of sea cucumber ( Cucumaria frondosa ) and wild blueberry ( Vaccinium angustifolium ) extracts have been reported recently. However, their anti-inflammatory activities and the mechanism of action against ALI are not fully elucidated. Thus, the present study aims to understand the mechanism of the anti-inflammatory activity of sea cucumber and wild blueberry extracts in the context of ALI. Experimental ALI was induced via intranasal lipopolysaccharide (LPS) instillation in C57BL/6 mice and the anti-inflammatory properties were determined by cytokine analysis, histological examination, western blot, and qRT-PCR. The results showed that oral supplementation of sea cucumber extracts repressed nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thereby downregulating the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) in the lung tissue and in the plasma. Wild blueberry extracts also suppressed the expression of IL-4. Furthermore, the combination of sea cucumber and wild blueberry extracts restrained MAPK signaling pathways by prominent attenuation of phosphorylation of NF-κB, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) while the levels of pro-inflammatory cytokines were significantly suppressed. Moreover, there was a significant and synergistic reduction in varying degrees of ALI lesions such as distorted parenchyma, increased alveoli thickness, lymphocyte and neutrophil infiltrations, fibrin deposition, pulmonary emphysema, pneumonia, intra-alveolar hemorrhage, and edema. The anti-inflammatory effect of the combination of sea cucumber and wild blueberry extracts is associated with suppressing MAPK and NF-κB signaling ... Article in Journal/Newspaper Cucumaria frondosa Directory of Open Access Journals: DOAJ Articles Molecules 29 7 1511 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cucumaria frondosa Vaccinium angustifolium ALI cytokine inflammation NF-κB Organic chemistry QD241-441 |
spellingShingle |
Cucumaria frondosa Vaccinium angustifolium ALI cytokine inflammation NF-κB Organic chemistry QD241-441 Oladapo F. Fagbohun Wasitha P. D. W. Thilakarathna Juan Zhou Christian Lehmann Guangling Jiao H. P. Vasantha Rupasinghe Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
topic_facet |
Cucumaria frondosa Vaccinium angustifolium ALI cytokine inflammation NF-κB Organic chemistry QD241-441 |
description |
Acute lung injury (ALI) represents a life-threatening condition with high morbidity and mortality despite modern mechanical ventilators and multiple pharmacological strategies. Therefore, there is a need to develop efficacious interventions with minimal side effects. The anti-inflammatory activities of sea cucumber ( Cucumaria frondosa ) and wild blueberry ( Vaccinium angustifolium ) extracts have been reported recently. However, their anti-inflammatory activities and the mechanism of action against ALI are not fully elucidated. Thus, the present study aims to understand the mechanism of the anti-inflammatory activity of sea cucumber and wild blueberry extracts in the context of ALI. Experimental ALI was induced via intranasal lipopolysaccharide (LPS) instillation in C57BL/6 mice and the anti-inflammatory properties were determined by cytokine analysis, histological examination, western blot, and qRT-PCR. The results showed that oral supplementation of sea cucumber extracts repressed nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thereby downregulating the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) in the lung tissue and in the plasma. Wild blueberry extracts also suppressed the expression of IL-4. Furthermore, the combination of sea cucumber and wild blueberry extracts restrained MAPK signaling pathways by prominent attenuation of phosphorylation of NF-κB, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) while the levels of pro-inflammatory cytokines were significantly suppressed. Moreover, there was a significant and synergistic reduction in varying degrees of ALI lesions such as distorted parenchyma, increased alveoli thickness, lymphocyte and neutrophil infiltrations, fibrin deposition, pulmonary emphysema, pneumonia, intra-alveolar hemorrhage, and edema. The anti-inflammatory effect of the combination of sea cucumber and wild blueberry extracts is associated with suppressing MAPK and NF-κB signaling ... |
format |
Article in Journal/Newspaper |
author |
Oladapo F. Fagbohun Wasitha P. D. W. Thilakarathna Juan Zhou Christian Lehmann Guangling Jiao H. P. Vasantha Rupasinghe |
author_facet |
Oladapo F. Fagbohun Wasitha P. D. W. Thilakarathna Juan Zhou Christian Lehmann Guangling Jiao H. P. Vasantha Rupasinghe |
author_sort |
Oladapo F. Fagbohun |
title |
Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
title_short |
Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
title_full |
Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
title_fullStr |
Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
title_full_unstemmed |
Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice |
title_sort |
sea cucumber and blueberry extracts suppress inflammation and reduce acute lung injury through the regulation of nf-κb/mapk/jnk signaling pathway in lipopolysaccharide-treated c57bl/6 mice |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/molecules29071511 https://doaj.org/article/51a7718b60cc418c9970ddd306e0ba3c |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_source |
Molecules, Vol 29, Iss 7, p 1511 (2024) |
op_relation |
https://www.mdpi.com/1420-3049/29/7/1511 https://doaj.org/toc/1420-3049 doi:10.3390/molecules29071511 1420-3049 https://doaj.org/article/51a7718b60cc418c9970ddd306e0ba3c |
op_doi |
https://doi.org/10.3390/molecules29071511 |
container_title |
Molecules |
container_volume |
29 |
container_issue |
7 |
container_start_page |
1511 |
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1810440891560624128 |