Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?
Abstract Background: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and...
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ftdoajarticles:oai:doaj.org/article:5160ffacd33d4f6e96f2017157a34c81 2023-05-15T15:11:29+02:00 Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? Adriana Simizo Eduardo S. Kitano Sávio S. Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Alexandre K. Tashima 2020-10-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0055 https://doaj.org/article/5160ffacd33d4f6e96f2017157a34c81 EN eng SciELO http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200055.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100331&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0055 https://doaj.org/article/5160ffacd33d4f6e96f2017157a34c81 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Bothrops atrox Venom Peptidomics Sexual dimorphism Disintegrin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0055 2022-12-30T20:35:46Z Abstract Background: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: We conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that phospholipase A2 and bradykinin potentiating peptides were the most abundant peptide families in both genders, whereas disintegrin levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides as the Ba1a, which contains the SVMP BATXSVMPII1 catalytic site, were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in disintegrin levels in the venoms of females that may result in distinct pathophysiology of envenomation. Further research is required to explore the potential biological implications of this finding. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Bothrops atrox Venom Peptidomics Sexual dimorphism Disintegrin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops atrox Venom Peptidomics Sexual dimorphism Disintegrin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Adriana Simizo Eduardo S. Kitano Sávio S. Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Alexandre K. Tashima Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
topic_facet |
Bothrops atrox Venom Peptidomics Sexual dimorphism Disintegrin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: We conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that phospholipase A2 and bradykinin potentiating peptides were the most abundant peptide families in both genders, whereas disintegrin levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides as the Ba1a, which contains the SVMP BATXSVMPII1 catalytic site, were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in disintegrin levels in the venoms of females that may result in distinct pathophysiology of envenomation. Further research is required to explore the potential biological implications of this finding. |
format |
Article in Journal/Newspaper |
author |
Adriana Simizo Eduardo S. Kitano Sávio S. Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Alexandre K. Tashima |
author_facet |
Adriana Simizo Eduardo S. Kitano Sávio S. Sant’Anna Kathleen Fernandes Grego Anita Mitico Tanaka-Azevedo Alexandre K. Tashima |
author_sort |
Adriana Simizo |
title |
Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
title_short |
Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
title_full |
Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
title_fullStr |
Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
title_full_unstemmed |
Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them? |
title_sort |
comparative gender peptidomics of bothrops atrox venoms: are there differences between them? |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0055 https://doaj.org/article/5160ffacd33d4f6e96f2017157a34c81 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20200055.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100331&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0055 https://doaj.org/article/5160ffacd33d4f6e96f2017157a34c81 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0055 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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1766342332308783104 |