Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum
The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 = 0.11 ± 0.02 µg prote...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:5137c0d79710490f89c624e01226e133 2023-05-15T15:06:22+02:00 Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum A Rojas A Feregrino C Ibarra-Alvarado MB Aguilar A Falcón E Heimer de la Cotera 2008-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992008000300009 https://doaj.org/article/5137c0d79710490f89c624e01226e133 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000300009 1678-9199 https://doaj.org/article/5137c0d79710490f89c624e01226e133 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 3, Pp 497-513 (2008) Conoidea Conus austini Conus spurius guinea pig ileum Polystira albida Turridae Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2008 ftdoajarticles https://doi.org/10.1590/S1678-91992008000300009 2022-12-31T09:08:52Z The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 = 0.11 ± 0.02 µg protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 µM), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 µM) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly inhibited contractions evoked by histamine (0.5 µM), particularly those from C. spurius and P. albida. These findings reveal the presence of antihistaminergic compounds not previously described in any conoidean venom. Finally, we found that only the extract prepared from C. spurius ducts decreased KCl (60 mM)-induced contractions, indicating that the venom of this snail contains compounds that block voltage-dependent Ca2+ or Na+ channels. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 14 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Conoidea Conus austini Conus spurius guinea pig ileum Polystira albida Turridae Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Conoidea Conus austini Conus spurius guinea pig ileum Polystira albida Turridae Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 A Rojas A Feregrino C Ibarra-Alvarado MB Aguilar A Falcón E Heimer de la Cotera Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
topic_facet |
Conoidea Conus austini Conus spurius guinea pig ileum Polystira albida Turridae Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 = 0.11 ± 0.02 µg protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 µM), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 µM) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly inhibited contractions evoked by histamine (0.5 µM), particularly those from C. spurius and P. albida. These findings reveal the presence of antihistaminergic compounds not previously described in any conoidean venom. Finally, we found that only the extract prepared from C. spurius ducts decreased KCl (60 mM)-induced contractions, indicating that the venom of this snail contains compounds that block voltage-dependent Ca2+ or Na+ channels. |
format |
Article in Journal/Newspaper |
author |
A Rojas A Feregrino C Ibarra-Alvarado MB Aguilar A Falcón E Heimer de la Cotera |
author_facet |
A Rojas A Feregrino C Ibarra-Alvarado MB Aguilar A Falcón E Heimer de la Cotera |
author_sort |
A Rojas |
title |
Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
title_short |
Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
title_full |
Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
title_fullStr |
Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
title_full_unstemmed |
Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum |
title_sort |
pharmacological characterization of venoms obtained from mexican toxoglossate gastropods on isolated guinea pig ileum |
publisher |
SciELO |
publishDate |
2008 |
url |
https://doi.org/10.1590/S1678-91992008000300009 https://doaj.org/article/5137c0d79710490f89c624e01226e133 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 3, Pp 497-513 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000300009 1678-9199 https://doaj.org/article/5137c0d79710490f89c624e01226e133 |
op_doi |
https://doi.org/10.1590/S1678-91992008000300009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
14 |
container_issue |
3 |
_version_ |
1766337986333507584 |