Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan
Objective: To study the seroprevalence of hepatitis B virus (HBV) and hepatitis delta virus (HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan. Methods: Blood samples were collected from non-hospitalized patients. Se...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:5118de1ad68b4353827e9ffe07f544cb 2023-05-15T15:09:29+02:00 Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan Ismail Jalil Muhammad Arshad Zara Rafaque Fazle Raziq Robina Wazir Sajid Malik Javid Iqbal Dasti 2016-07-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.05.007 https://doaj.org/article/5118de1ad68b4353827e9ffe07f544cb EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116300533 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.05.007 https://doaj.org/article/5118de1ad68b4353827e9ffe07f544cb Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 7, Pp 609-613 (2016) HBV HCV HDV Risk factors Co-infection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.05.007 2022-12-31T14:12:15Z Objective: To study the seroprevalence of hepatitis B virus (HBV) and hepatitis delta virus (HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan. Methods: Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel electrophoresis. Results: Altogether, 946 blood samples were screened, overall percentage of HBsAg-positive patients remained 22.41% (prevalence: 224.10/1000; CI: 0.1975 ± 0.2507) with the highest incidence rates among relatively younger age groups (20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1000; CI: 0.0318 ± 0.0617. In HBsAg-positive patients, anti-HBc-total was detected in 86.79% while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise (62%), anorexia (66%) and fatigue (73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection (χ2 = 95.23; df = 11; P < 0.0001). Conclusions: Overall, this study confirmed higher prevalence of active HBV/HDV infection, among young patients from Khyber Pakhtunkhwa region having no prior history of viral hepatitis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 7 609 613 |
institution |
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collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HBV HCV HDV Risk factors Co-infection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
HBV HCV HDV Risk factors Co-infection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ismail Jalil Muhammad Arshad Zara Rafaque Fazle Raziq Robina Wazir Sajid Malik Javid Iqbal Dasti Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
topic_facet |
HBV HCV HDV Risk factors Co-infection Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study the seroprevalence of hepatitis B virus (HBV) and hepatitis delta virus (HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan. Methods: Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel electrophoresis. Results: Altogether, 946 blood samples were screened, overall percentage of HBsAg-positive patients remained 22.41% (prevalence: 224.10/1000; CI: 0.1975 ± 0.2507) with the highest incidence rates among relatively younger age groups (20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1000; CI: 0.0318 ± 0.0617. In HBsAg-positive patients, anti-HBc-total was detected in 86.79% while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise (62%), anorexia (66%) and fatigue (73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection (χ2 = 95.23; df = 11; P < 0.0001). Conclusions: Overall, this study confirmed higher prevalence of active HBV/HDV infection, among young patients from Khyber Pakhtunkhwa region having no prior history of viral hepatitis. |
format |
Article in Journal/Newspaper |
author |
Ismail Jalil Muhammad Arshad Zara Rafaque Fazle Raziq Robina Wazir Sajid Malik Javid Iqbal Dasti |
author_facet |
Ismail Jalil Muhammad Arshad Zara Rafaque Fazle Raziq Robina Wazir Sajid Malik Javid Iqbal Dasti |
author_sort |
Ismail Jalil |
title |
Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
title_short |
Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
title_full |
Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
title_fullStr |
Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
title_full_unstemmed |
Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan |
title_sort |
seroprevalence of hdv among non-hospitalized hbsag positive patients from kpk-region of pakistan |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.05.007 https://doaj.org/article/5118de1ad68b4353827e9ffe07f544cb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 7, Pp 609-613 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116300533 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.05.007 https://doaj.org/article/5118de1ad68b4353827e9ffe07f544cb |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.05.007 |
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Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
7 |
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609 |
op_container_end_page |
613 |
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