Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark
Objective: To evaluate antioxidant, cytotoxic, and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr. Methods: Gas chromatography-mass spectrometry (GC-MS) and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia. Bi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:5114d99e1a8d480b96dc1535bf929196 2023-05-15T15:16:36+02:00 Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark Maria Carmen S. Tan Mary Stephanie S. Carranza Virgilio C Linis Raymond S Malabed Yves Ira A. Reyes Francisco C Franco Glenn G Oyong 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.326100 https://doaj.org/article/5114d99e1a8d480b96dc1535bf929196 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=10;spage=460;epage=468;aulast=Tan https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.326100 https://doaj.org/article/5114d99e1a8d480b96dc1535bf929196 Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 10, Pp 460-468 (2021) alstonia parvifolia merr. naja philippinensis taylor gas chromatography-electron ionization-mass spectrometry secretory phospholipase a2 cytotoxicity assay anti-venom Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.326100 2022-12-30T22:08:54Z Objective: To evaluate antioxidant, cytotoxic, and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr. Methods: Gas chromatography-mass spectrometry (GC-MS) and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia. Biochemical characterization was evaluated using an inhibitory phospholipase A2 (PLA2) assay, DPPH, and cytotoxicity assays. Using the constituents listed in the GC-MS analyses, molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA2 isoforms. Results: GC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin (14.89%), γ-sitosterol (10.44%), 3-O-methyl-D-glucose (5.88%), 3,5-dimethoxy-4-hydroxyphenylacetic acid (5.30%), (2α,5α)-17-methoxyaspidofractinin-3-one (AFM) (4.08%), and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1],[4]thiazepine (HPT) (1.37%). The principal elemental components of Alstonia parvifolia were Ca (4.012%) and K (1.496%), as exhibited by energy dispersive X-ray examination. Alstonia parvifolia showed significant free radical scavenging ability (IC50: 0.287 mg/mL) and was non-cytotoxic to normal HDFn cells (IC50 >100 μg/mL). Moreover, Alstonia parvifolia was favorably cytotoxic to MCF-7 (IC50: 4.42 μg/mL), followed by H69PR, HT-29, and THP-1, with IC50 values of 4.94, 5.07, and 6.27 μg/mL, respectively. Alstonia parvifolia also displayed notable inhibition against PLA2 activity of Naja philippinensis Taylor venom with IC50 of (15.2 ± 1.8) μg/mL. Docking and cluster analyses projected negative binding energies from AFM (-6.36 to -9.68 kcal/mol), HPT (-7.38 to -9.77 kcal/ mol), and acetylmarinobufogenin (-7.22 to -9.59 kcal/mol). These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA2 homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid residues. Conclusions: The bark ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 10 460 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
alstonia parvifolia merr. naja philippinensis taylor gas chromatography-electron ionization-mass spectrometry secretory phospholipase a2 cytotoxicity assay anti-venom Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
alstonia parvifolia merr. naja philippinensis taylor gas chromatography-electron ionization-mass spectrometry secretory phospholipase a2 cytotoxicity assay anti-venom Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maria Carmen S. Tan Mary Stephanie S. Carranza Virgilio C Linis Raymond S Malabed Yves Ira A. Reyes Francisco C Franco Glenn G Oyong Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
topic_facet |
alstonia parvifolia merr. naja philippinensis taylor gas chromatography-electron ionization-mass spectrometry secretory phospholipase a2 cytotoxicity assay anti-venom Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate antioxidant, cytotoxic, and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr. Methods: Gas chromatography-mass spectrometry (GC-MS) and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia. Biochemical characterization was evaluated using an inhibitory phospholipase A2 (PLA2) assay, DPPH, and cytotoxicity assays. Using the constituents listed in the GC-MS analyses, molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA2 isoforms. Results: GC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin (14.89%), γ-sitosterol (10.44%), 3-O-methyl-D-glucose (5.88%), 3,5-dimethoxy-4-hydroxyphenylacetic acid (5.30%), (2α,5α)-17-methoxyaspidofractinin-3-one (AFM) (4.08%), and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1],[4]thiazepine (HPT) (1.37%). The principal elemental components of Alstonia parvifolia were Ca (4.012%) and K (1.496%), as exhibited by energy dispersive X-ray examination. Alstonia parvifolia showed significant free radical scavenging ability (IC50: 0.287 mg/mL) and was non-cytotoxic to normal HDFn cells (IC50 >100 μg/mL). Moreover, Alstonia parvifolia was favorably cytotoxic to MCF-7 (IC50: 4.42 μg/mL), followed by H69PR, HT-29, and THP-1, with IC50 values of 4.94, 5.07, and 6.27 μg/mL, respectively. Alstonia parvifolia also displayed notable inhibition against PLA2 activity of Naja philippinensis Taylor venom with IC50 of (15.2 ± 1.8) μg/mL. Docking and cluster analyses projected negative binding energies from AFM (-6.36 to -9.68 kcal/mol), HPT (-7.38 to -9.77 kcal/ mol), and acetylmarinobufogenin (-7.22 to -9.59 kcal/mol). These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA2 homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid residues. Conclusions: The bark ... |
format |
Article in Journal/Newspaper |
author |
Maria Carmen S. Tan Mary Stephanie S. Carranza Virgilio C Linis Raymond S Malabed Yves Ira A. Reyes Francisco C Franco Glenn G Oyong |
author_facet |
Maria Carmen S. Tan Mary Stephanie S. Carranza Virgilio C Linis Raymond S Malabed Yves Ira A. Reyes Francisco C Franco Glenn G Oyong |
author_sort |
Maria Carmen S. Tan |
title |
Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
title_short |
Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
title_full |
Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
title_fullStr |
Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
title_full_unstemmed |
Antioxidant, cytotoxic, and anti-venom activity of Alstonia parvifolia Merr. Bark |
title_sort |
antioxidant, cytotoxic, and anti-venom activity of alstonia parvifolia merr. bark |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.326100 https://doaj.org/article/5114d99e1a8d480b96dc1535bf929196 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 10, Pp 460-468 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=10;spage=460;epage=468;aulast=Tan https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.326100 https://doaj.org/article/5114d99e1a8d480b96dc1535bf929196 |
op_doi |
https://doi.org/10.4103/2221-1691.326100 |
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Asian Pacific Journal of Tropical Biomedicine |
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11 |
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10 |
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460 |
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1766346889108652032 |