A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity
Abstract Background The Antarctic continent is a source of extreme microorganisms. Millions of years of isolation have produced unique biodiversity with adaptive responses to its extreme environment. Although the Antarctic climate is mainly cold, the presence of several geothermal sites, including t...
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ftdoajarticles:oai:doaj.org/article:51123f0a542e4e6e9a17417527931234 2023-05-15T13:56:42+02:00 A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity Patricio A. M. Flores Daniela N. Correa-Llantén Jenny M. Blamey 2018-12-01T00:00:00Z https://doi.org/10.1186/s40659-018-0206-3 https://doaj.org/article/51123f0a542e4e6e9a17417527931234 EN eng BMC http://link.springer.com/article/10.1186/s40659-018-0206-3 https://doaj.org/toc/0717-6287 doi:10.1186/s40659-018-0206-3 0717-6287 https://doaj.org/article/51123f0a542e4e6e9a17417527931234 Biological Research, Vol 51, Iss 1, Pp 1-7 (2018) Bacteria Bacillus Glutamate dehydrogenase Biotechnology Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.1186/s40659-018-0206-3 2022-12-31T12:36:22Z Abstract Background The Antarctic continent is a source of extreme microorganisms. Millions of years of isolation have produced unique biodiversity with adaptive responses to its extreme environment. Although the Antarctic climate is mainly cold, the presence of several geothermal sites, including thermal springs, fumaroles, hot soils and hydrothermal vents, provides ideal environments for the development of thermophilic and hyperthermophilic microorganisms. Their enzymes, called thermoenzymes, are the focus of interest in both academic and industrial research, mainly due to their high thermal activity and stability. Glutamate dehydrogenase, is an enzyme that plays a key role in the metabolism of carbon and nitrogen catalyzing reversibly the oxidative deamination of glutamate to alpha-ketoglutarate and ammonium. It belongs to the family of oxidoreductases, is widely distributed and it has been highly regarded for use as biosensors, particularly for their specificity and ability to operate in photochemical and electrochemical systems. However, the use of enzymes as biosensors is relatively problematic due to their instability to high temperatures, organic solvents and denaturing agents. The purpose of this study is to present the partial characterization of a thermophilic microorganism isolated from Deception Island, Antarctica, that displays glutamate dehydrogenase activity. Results In this work, we report the isolation of a thermophilic microorganism called PID15 from samples of Deception Island collected during the Antarctic Scientific Expedition ECA 46. This microorganism is a thermophile that grows optimally at 50 °C and pH 8.0. Scanning electron microscopy shows rod cells of 2.0 to 8.0 µm of length. Phylogenetic analysis of 16S rRNA gene revealed that this microorganism is closely related to Bacillus gelatini. This microorganism contains a thermostable glutamate dehydrogenase with optimal activity at pH 8.0 and temperatures for its activity from 37 to 50 °C, range of temperature of interest for ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Deception Island Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Biological Research 51 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacteria Bacillus Glutamate dehydrogenase Biotechnology Biology (General) QH301-705.5 |
spellingShingle |
Bacteria Bacillus Glutamate dehydrogenase Biotechnology Biology (General) QH301-705.5 Patricio A. M. Flores Daniela N. Correa-Llantén Jenny M. Blamey A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
topic_facet |
Bacteria Bacillus Glutamate dehydrogenase Biotechnology Biology (General) QH301-705.5 |
description |
Abstract Background The Antarctic continent is a source of extreme microorganisms. Millions of years of isolation have produced unique biodiversity with adaptive responses to its extreme environment. Although the Antarctic climate is mainly cold, the presence of several geothermal sites, including thermal springs, fumaroles, hot soils and hydrothermal vents, provides ideal environments for the development of thermophilic and hyperthermophilic microorganisms. Their enzymes, called thermoenzymes, are the focus of interest in both academic and industrial research, mainly due to their high thermal activity and stability. Glutamate dehydrogenase, is an enzyme that plays a key role in the metabolism of carbon and nitrogen catalyzing reversibly the oxidative deamination of glutamate to alpha-ketoglutarate and ammonium. It belongs to the family of oxidoreductases, is widely distributed and it has been highly regarded for use as biosensors, particularly for their specificity and ability to operate in photochemical and electrochemical systems. However, the use of enzymes as biosensors is relatively problematic due to their instability to high temperatures, organic solvents and denaturing agents. The purpose of this study is to present the partial characterization of a thermophilic microorganism isolated from Deception Island, Antarctica, that displays glutamate dehydrogenase activity. Results In this work, we report the isolation of a thermophilic microorganism called PID15 from samples of Deception Island collected during the Antarctic Scientific Expedition ECA 46. This microorganism is a thermophile that grows optimally at 50 °C and pH 8.0. Scanning electron microscopy shows rod cells of 2.0 to 8.0 µm of length. Phylogenetic analysis of 16S rRNA gene revealed that this microorganism is closely related to Bacillus gelatini. This microorganism contains a thermostable glutamate dehydrogenase with optimal activity at pH 8.0 and temperatures for its activity from 37 to 50 °C, range of temperature of interest for ... |
format |
Article in Journal/Newspaper |
author |
Patricio A. M. Flores Daniela N. Correa-Llantén Jenny M. Blamey |
author_facet |
Patricio A. M. Flores Daniela N. Correa-Llantén Jenny M. Blamey |
author_sort |
Patricio A. M. Flores |
title |
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
title_short |
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
title_full |
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
title_fullStr |
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
title_full_unstemmed |
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |
title_sort |
thermophilic microorganism from deception island, antarctica with a thermostable glutamate dehydrogenase activity |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s40659-018-0206-3 https://doaj.org/article/51123f0a542e4e6e9a17417527931234 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic The Antarctic Deception Island |
geographic_facet |
Antarctic The Antarctic Deception Island |
genre |
Antarc* Antarctic Antarctica Deception Island |
genre_facet |
Antarc* Antarctic Antarctica Deception Island |
op_source |
Biological Research, Vol 51, Iss 1, Pp 1-7 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s40659-018-0206-3 https://doaj.org/toc/0717-6287 doi:10.1186/s40659-018-0206-3 0717-6287 https://doaj.org/article/51123f0a542e4e6e9a17417527931234 |
op_doi |
https://doi.org/10.1186/s40659-018-0206-3 |
container_title |
Biological Research |
container_volume |
51 |
container_issue |
1 |
_version_ |
1766264282602799104 |