A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella.
Brucella are highly infectious bacterial pathogens responsible for a severely debilitating zoonosis called brucellosis. Half of the human population worldwide is considered to live at risk of exposure, mostly in the poorest rural areas of the world. Prompt diagnosis of brucellosis is essential to pr...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:50ff8634f1134ec2913508232483a142 2023-05-15T15:11:14+02:00 A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. Jennifer Mesureur Sandrine Arend Béatrice Cellière Priscillia Courault Pierre-Jean Cotte-Pattat Heather Totty Parampal Deol Virginie Mick Victoria Girard Joanne Touchberry Vanessa Burrowes Jean-Philippe Lavigne David O'Callaghan Valérie Monnin Anne Keriel 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006874 https://doaj.org/article/50ff8634f1134ec2913508232483a142 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6207331?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006874 https://doaj.org/article/50ff8634f1134ec2913508232483a142 PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006874 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006874 2022-12-30T21:04:39Z Brucella are highly infectious bacterial pathogens responsible for a severely debilitating zoonosis called brucellosis. Half of the human population worldwide is considered to live at risk of exposure, mostly in the poorest rural areas of the world. Prompt diagnosis of brucellosis is essential to prevent complications and to control epidemiology outbreaks, but identification of Brucella isolates may be hampered by the lack of rapid and cost-effective methods. Nowadays, many clinical microbiology laboratories use Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) for routine identification. However, lack of reference spectra in the currently commercialized databases does not allow the identification of Brucella isolates. In this work, we constructed a Brucella MALDI-TOF MS reference database using VITEK MS. We generated 590 spectra from 84 different strains (including rare or atypical isolates) to cover this bacterial genus. We then applied a novel biomathematical approach to discriminate different species. This allowed accurate identification of Brucella isolates at the genus level with no misidentifications, in particular as the closely related and less pathogenic Ochrobactrum genus. The main zoonotic species (B. melitensis, B. abortus and B. suis) could also be identified at the species level with an accuracy of 100%, 92.9% and 100%, respectively. This MALDI-TOF reference database will be the first Brucella database validated for diagnostic and accessible to all VITEK MS users in routine. This will improve the diagnosis and control of brucellosis by allowing a rapid identification of these pathogens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 10 e0006874 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jennifer Mesureur Sandrine Arend Béatrice Cellière Priscillia Courault Pierre-Jean Cotte-Pattat Heather Totty Parampal Deol Virginie Mick Victoria Girard Joanne Touchberry Vanessa Burrowes Jean-Philippe Lavigne David O'Callaghan Valérie Monnin Anne Keriel A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Brucella are highly infectious bacterial pathogens responsible for a severely debilitating zoonosis called brucellosis. Half of the human population worldwide is considered to live at risk of exposure, mostly in the poorest rural areas of the world. Prompt diagnosis of brucellosis is essential to prevent complications and to control epidemiology outbreaks, but identification of Brucella isolates may be hampered by the lack of rapid and cost-effective methods. Nowadays, many clinical microbiology laboratories use Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) for routine identification. However, lack of reference spectra in the currently commercialized databases does not allow the identification of Brucella isolates. In this work, we constructed a Brucella MALDI-TOF MS reference database using VITEK MS. We generated 590 spectra from 84 different strains (including rare or atypical isolates) to cover this bacterial genus. We then applied a novel biomathematical approach to discriminate different species. This allowed accurate identification of Brucella isolates at the genus level with no misidentifications, in particular as the closely related and less pathogenic Ochrobactrum genus. The main zoonotic species (B. melitensis, B. abortus and B. suis) could also be identified at the species level with an accuracy of 100%, 92.9% and 100%, respectively. This MALDI-TOF reference database will be the first Brucella database validated for diagnostic and accessible to all VITEK MS users in routine. This will improve the diagnosis and control of brucellosis by allowing a rapid identification of these pathogens. |
format |
Article in Journal/Newspaper |
author |
Jennifer Mesureur Sandrine Arend Béatrice Cellière Priscillia Courault Pierre-Jean Cotte-Pattat Heather Totty Parampal Deol Virginie Mick Victoria Girard Joanne Touchberry Vanessa Burrowes Jean-Philippe Lavigne David O'Callaghan Valérie Monnin Anne Keriel |
author_facet |
Jennifer Mesureur Sandrine Arend Béatrice Cellière Priscillia Courault Pierre-Jean Cotte-Pattat Heather Totty Parampal Deol Virginie Mick Victoria Girard Joanne Touchberry Vanessa Burrowes Jean-Philippe Lavigne David O'Callaghan Valérie Monnin Anne Keriel |
author_sort |
Jennifer Mesureur |
title |
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
title_short |
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
title_full |
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
title_fullStr |
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
title_full_unstemmed |
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. |
title_sort |
maldi-tof ms database with broad genus coverage for species-level identification of brucella. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006874 https://doaj.org/article/50ff8634f1134ec2913508232483a142 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006874 (2018) |
op_relation |
http://europepmc.org/articles/PMC6207331?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006874 https://doaj.org/article/50ff8634f1134ec2913508232483a142 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006874 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
10 |
container_start_page |
e0006874 |
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1766342123480678400 |