Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1.
Cholera continues to be an important public health concern in developing countries where proper hygiene and sanitation are compromised. This severe diarrheal disease is caused by the Gram-negative pathogen Vibrio cholerae belonging to serogroups O1 and O139. Cholera toxin (CT) is the prime virulence...
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ftdoajarticles:oai:doaj.org/article:50ad3bfd8b32493486f25b8dd9eebc44 2023-05-15T15:12:30+02:00 Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. Arindam Naha Rahul Shubhra Mandal Prosenjit Samanta Rudra Narayan Saha Sreeja Shaw Amit Ghosh Nabendu Sekhar Chatterjee Pujarini Dutta Keinosuke Okamoto Shanta Dutta Asish Kumar Mukhopadhyay 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008128 https://doaj.org/article/50ad3bfd8b32493486f25b8dd9eebc44 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008128 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008128 https://doaj.org/article/50ad3bfd8b32493486f25b8dd9eebc44 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008128 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008128 2022-12-31T12:49:08Z Cholera continues to be an important public health concern in developing countries where proper hygiene and sanitation are compromised. This severe diarrheal disease is caused by the Gram-negative pathogen Vibrio cholerae belonging to serogroups O1 and O139. Cholera toxin (CT) is the prime virulence factor and is directly responsible for the disease manifestation. The ctxB gene encodes cholera toxin B subunit (CTB) whereas the A subunit (CTA) is the product of ctxA gene. Enzymatic action of CT depends on binding of B pentamers to the lipid-based receptor ganglioside GM1. In recent years, emergence of V. cholerae Haitian variant strains with ctxB7 allele and their rapid spread throughout the globe has been linked to various cholera outbreaks in Africa and Asia. These strains produce classical type (WT) CTB except for an additional mutation in the signal sequence region where an asparagine (N) residue replaces a histidine (H) at the 20th amino acid position (H20N) of CTB precursor (pre-CTB). Here we report that Haitian variant V. cholerae O1 strains isolated in Kolkata produced higher amount of CT compared to contemporary O1 El Tor variant strains under in vitro virulence inducing conditions. We observed that the ctxB7 allele, itself plays a pivotal role in higher CT production. Based on our in silico analysis, we hypothesized that higher accumulation of toxin subunits from ctxB7 allele might be attributed to the structural alteration at the CTB signal peptide region of pre-H20N CTB. Overall, this study provides plausible explanation regarding the hypertoxigenic phenotype of the Haitian variant strains which have spread globally, possibly through positive selection for increased pathogenic traits. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008128 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Arindam Naha Rahul Shubhra Mandal Prosenjit Samanta Rudra Narayan Saha Sreeja Shaw Amit Ghosh Nabendu Sekhar Chatterjee Pujarini Dutta Keinosuke Okamoto Shanta Dutta Asish Kumar Mukhopadhyay Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cholera continues to be an important public health concern in developing countries where proper hygiene and sanitation are compromised. This severe diarrheal disease is caused by the Gram-negative pathogen Vibrio cholerae belonging to serogroups O1 and O139. Cholera toxin (CT) is the prime virulence factor and is directly responsible for the disease manifestation. The ctxB gene encodes cholera toxin B subunit (CTB) whereas the A subunit (CTA) is the product of ctxA gene. Enzymatic action of CT depends on binding of B pentamers to the lipid-based receptor ganglioside GM1. In recent years, emergence of V. cholerae Haitian variant strains with ctxB7 allele and their rapid spread throughout the globe has been linked to various cholera outbreaks in Africa and Asia. These strains produce classical type (WT) CTB except for an additional mutation in the signal sequence region where an asparagine (N) residue replaces a histidine (H) at the 20th amino acid position (H20N) of CTB precursor (pre-CTB). Here we report that Haitian variant V. cholerae O1 strains isolated in Kolkata produced higher amount of CT compared to contemporary O1 El Tor variant strains under in vitro virulence inducing conditions. We observed that the ctxB7 allele, itself plays a pivotal role in higher CT production. Based on our in silico analysis, we hypothesized that higher accumulation of toxin subunits from ctxB7 allele might be attributed to the structural alteration at the CTB signal peptide region of pre-H20N CTB. Overall, this study provides plausible explanation regarding the hypertoxigenic phenotype of the Haitian variant strains which have spread globally, possibly through positive selection for increased pathogenic traits. |
format |
Article in Journal/Newspaper |
author |
Arindam Naha Rahul Shubhra Mandal Prosenjit Samanta Rudra Narayan Saha Sreeja Shaw Amit Ghosh Nabendu Sekhar Chatterjee Pujarini Dutta Keinosuke Okamoto Shanta Dutta Asish Kumar Mukhopadhyay |
author_facet |
Arindam Naha Rahul Shubhra Mandal Prosenjit Samanta Rudra Narayan Saha Sreeja Shaw Amit Ghosh Nabendu Sekhar Chatterjee Pujarini Dutta Keinosuke Okamoto Shanta Dutta Asish Kumar Mukhopadhyay |
author_sort |
Arindam Naha |
title |
Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
title_short |
Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
title_full |
Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
title_fullStr |
Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
title_full_unstemmed |
Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. |
title_sort |
deciphering the possible role of ctxb7 allele on higher production of cholera toxin by haitian variant vibrio cholerae o1. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008128 https://doaj.org/article/50ad3bfd8b32493486f25b8dd9eebc44 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008128 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008128 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008128 https://doaj.org/article/50ad3bfd8b32493486f25b8dd9eebc44 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008128 |
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PLOS Neglected Tropical Diseases |
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e0008128 |
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