Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran
Tehran’s actual prevalence of Chlamydia trachomatis (CT) and its genotypes are still unclear. Molecular typing of CT strains can provide essential epidemiological knowledge and contribute to improved control measures. In this study, we aimed to determine the prevalence of CT and its genotypes in the...
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ftdoajarticles:oai:doaj.org/article:509500f1e3294b9593595e43eefc8951 2024-09-09T19:25:51+00:00 Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran Mohammadreza Rajabpour Amir Darb Emamie Mohammad Reza Pourmand 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/8845565 https://doaj.org/article/509500f1e3294b9593595e43eefc8951 EN eng Wiley http://dx.doi.org/10.1155/2023/8845565 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8845565 https://doaj.org/article/509500f1e3294b9593595e43eefc8951 Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/8845565 2024-08-05T17:48:35Z Tehran’s actual prevalence of Chlamydia trachomatis (CT) and its genotypes are still unclear. Molecular typing of CT strains can provide essential epidemiological knowledge and contribute to improved control measures. In this study, we aimed to determine the prevalence of CT and its genotypes in the endocervical infections of females who attended the gynecology and infertility clinics in Tehran. A total of 291 women were tested for chlamydial infection by in-house PCR using specific primers for the CT cryptic plasmid. Nested PCR for amplification of the ompA gene in positive samples was carried out, genotyping was performed by sequencing this gene, and further phylogenetic analysis was conducted. Sexual infection by CT was observed in 10.3% (30/291) of the subjects, and the mean age of patients was 30.4. The ompA gene was sequenced in 27 samples, revealing E genotypes 40.7%, (n = 11), F 25.9%, (n = 7), G 18.5%, (n = 5), D 11.1%, (n = 3), and K 3.7%, (n = 1). This study emphasizes the importance of the diversity among CT genotypes in our studied population and the need for wide-screening the neglected bacterial infection among women in Tehran. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Mohammadreza Rajabpour Amir Darb Emamie Mohammad Reza Pourmand Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Tehran’s actual prevalence of Chlamydia trachomatis (CT) and its genotypes are still unclear. Molecular typing of CT strains can provide essential epidemiological knowledge and contribute to improved control measures. In this study, we aimed to determine the prevalence of CT and its genotypes in the endocervical infections of females who attended the gynecology and infertility clinics in Tehran. A total of 291 women were tested for chlamydial infection by in-house PCR using specific primers for the CT cryptic plasmid. Nested PCR for amplification of the ompA gene in positive samples was carried out, genotyping was performed by sequencing this gene, and further phylogenetic analysis was conducted. Sexual infection by CT was observed in 10.3% (30/291) of the subjects, and the mean age of patients was 30.4. The ompA gene was sequenced in 27 samples, revealing E genotypes 40.7%, (n = 11), F 25.9%, (n = 7), G 18.5%, (n = 5), D 11.1%, (n = 3), and K 3.7%, (n = 1). This study emphasizes the importance of the diversity among CT genotypes in our studied population and the need for wide-screening the neglected bacterial infection among women in Tehran. |
format |
Article in Journal/Newspaper |
author |
Mohammadreza Rajabpour Amir Darb Emamie Mohammad Reza Pourmand |
author_facet |
Mohammadreza Rajabpour Amir Darb Emamie Mohammad Reza Pourmand |
author_sort |
Mohammadreza Rajabpour |
title |
Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
title_short |
Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
title_full |
Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
title_fullStr |
Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
title_full_unstemmed |
Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran |
title_sort |
evaluation of chlamydia trachomatis genotypes in endocervical specimens by sequence analysis of ompa gene among women in tehran |
publisher |
Wiley |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/8845565 https://doaj.org/article/509500f1e3294b9593595e43eefc8951 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/8845565 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8845565 https://doaj.org/article/509500f1e3294b9593595e43eefc8951 |
op_doi |
https://doi.org/10.1155/2023/8845565 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
6 |
_version_ |
1809895565108969472 |