Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR
Amoebic gill disease (AGD) is the primary health concern for Atlantic salmon Salmo salar farmed in Tasmania, Australia. Neoparamoeba perurans is the aetiological agent of AGD; however, a diversity of other amoebae colonise the gills, and their role in AGD is unknown. Previous studies which document...
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ftdoajarticles:oai:doaj.org/article:504ca294cf9c4115ac59b00cc8297848 2023-05-15T15:30:30+02:00 Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR English, CJ Swords, F Downes, JK Ruane, NM Botwright, NA Taylor, RS Barnes, AC Wynne, JW Lima, PC Cook, MT 2019-08-01T00:00:00Z https://doi.org/10.3354/aei00325 https://doaj.org/article/504ca294cf9c4115ac59b00cc8297848 EN eng Inter-Research https://www.int-res.com/abstracts/aei/v11/p405-415/ https://doaj.org/toc/1869-215X https://doaj.org/toc/1869-7534 1869-215X 1869-7534 doi:10.3354/aei00325 https://doaj.org/article/504ca294cf9c4115ac59b00cc8297848 Aquaculture Environment Interactions, Vol 11, Pp 405-415 (2019) Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 article 2019 ftdoajarticles https://doi.org/10.3354/aei00325 2022-12-31T03:54:02Z Amoebic gill disease (AGD) is the primary health concern for Atlantic salmon Salmo salar farmed in Tasmania, Australia. Neoparamoeba perurans is the aetiological agent of AGD; however, a diversity of other amoebae colonise the gills, and their role in AGD is unknown. Previous studies which document these accompanying amoebae on AGD-affected farmed Atlantic salmon relied on culture-based techniques which do not accurately determine the prevalence and abundance of these species nor whether they correlate with AGD pathology. Drawing on our previous culture-based study, here we develop and apply 5 new Taqman quantitative PCR (qPCR) assays to profile the prevalence of multiple amoeba species on the gills of AGD-affected Atlantic salmon held at 2 Tasmanian farm sites over a 1 yr period. The prevalence and abundance of N. perurans was also assessed using a previously established qPCR method. N. perurans was the dominant species, and its abundance positively correlated with the progression of gross gill pathology. Only a small number of sporadic detections of Pseudoparamoeba and Vannellida species were observed. Nolandella spp. was the notable exception, as it was the most prevalent amoeba (92%) at 1 site at 1 sample time, during which no N. perurans were detected on gills but low levels of gross gill pathology were observed. N. perurans is the predominant species and primary pathogen of AGD; however, there were instances when they were not detected on diseased gills and Nolandella spp. were highly prevalent. The significance of Nolandella spp. in relation to AGD is not yet understood. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Aquaculture Environment Interactions 11 405 415 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 |
spellingShingle |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 English, CJ Swords, F Downes, JK Ruane, NM Botwright, NA Taylor, RS Barnes, AC Wynne, JW Lima, PC Cook, MT Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
topic_facet |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 |
description |
Amoebic gill disease (AGD) is the primary health concern for Atlantic salmon Salmo salar farmed in Tasmania, Australia. Neoparamoeba perurans is the aetiological agent of AGD; however, a diversity of other amoebae colonise the gills, and their role in AGD is unknown. Previous studies which document these accompanying amoebae on AGD-affected farmed Atlantic salmon relied on culture-based techniques which do not accurately determine the prevalence and abundance of these species nor whether they correlate with AGD pathology. Drawing on our previous culture-based study, here we develop and apply 5 new Taqman quantitative PCR (qPCR) assays to profile the prevalence of multiple amoeba species on the gills of AGD-affected Atlantic salmon held at 2 Tasmanian farm sites over a 1 yr period. The prevalence and abundance of N. perurans was also assessed using a previously established qPCR method. N. perurans was the dominant species, and its abundance positively correlated with the progression of gross gill pathology. Only a small number of sporadic detections of Pseudoparamoeba and Vannellida species were observed. Nolandella spp. was the notable exception, as it was the most prevalent amoeba (92%) at 1 site at 1 sample time, during which no N. perurans were detected on gills but low levels of gross gill pathology were observed. N. perurans is the predominant species and primary pathogen of AGD; however, there were instances when they were not detected on diseased gills and Nolandella spp. were highly prevalent. The significance of Nolandella spp. in relation to AGD is not yet understood. |
format |
Article in Journal/Newspaper |
author |
English, CJ Swords, F Downes, JK Ruane, NM Botwright, NA Taylor, RS Barnes, AC Wynne, JW Lima, PC Cook, MT |
author_facet |
English, CJ Swords, F Downes, JK Ruane, NM Botwright, NA Taylor, RS Barnes, AC Wynne, JW Lima, PC Cook, MT |
author_sort |
English, CJ |
title |
Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
title_short |
Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
title_full |
Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
title_fullStr |
Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
title_full_unstemmed |
Prevalence of six amoeba species colonising the gills of farmed Atlantic salmon with amoebic gill disease (AGD) using qPCR |
title_sort |
prevalence of six amoeba species colonising the gills of farmed atlantic salmon with amoebic gill disease (agd) using qpcr |
publisher |
Inter-Research |
publishDate |
2019 |
url |
https://doi.org/10.3354/aei00325 https://doaj.org/article/504ca294cf9c4115ac59b00cc8297848 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquaculture Environment Interactions, Vol 11, Pp 405-415 (2019) |
op_relation |
https://www.int-res.com/abstracts/aei/v11/p405-415/ https://doaj.org/toc/1869-215X https://doaj.org/toc/1869-7534 1869-215X 1869-7534 doi:10.3354/aei00325 https://doaj.org/article/504ca294cf9c4115ac59b00cc8297848 |
op_doi |
https://doi.org/10.3354/aei00325 |
container_title |
Aquaculture Environment Interactions |
container_volume |
11 |
container_start_page |
405 |
op_container_end_page |
415 |
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1766360959852478464 |