Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahy...

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Bibliographic Details
Published in:Frontiers in Bioengineering and Biotechnology
Main Authors: Yangxin Wang, Ningning Zhang, Deming Tan, Zhenhui Qi, Changzhu Wu
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2020
Subjects:
metal–organic frameworks
enzymatic reactions
size-selective catalysis
heterogeneous catalysis
biocatalysis
Biotechnology
TP248.13-248.65
Antarc*
Antarctica
Online Access:https://doi.org/10.3389/fbioe.2020.00714
https://doaj.org/article/50143f10908b4a8483e9be13d8c06ebb
Description
Summary:In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

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