Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species.
The highly repetitive DNA sequence of centromeric heterochromatin is an effective molecular cytogenetic marker for investigating genomic compartmentalization between macrochromosomes and microchromosomes in birds. We isolated four repetitive sequence families of centromeric heterochromatin from thre...
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ftdoajarticles:oai:doaj.org/article:50020f8da050445eba8bc5b0cf1dfb32 2023-05-15T13:30:10+02:00 Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. Yoshinobu Uno Chizuko Nishida Ayano Hata Satoshi Ishishita Yoichi Matsuda 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0214028 https://doaj.org/article/50020f8da050445eba8bc5b0cf1dfb32 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0214028 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0214028 https://doaj.org/article/50020f8da050445eba8bc5b0cf1dfb32 PLoS ONE, Vol 14, Iss 3, p e0214028 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0214028 2022-12-31T11:17:19Z The highly repetitive DNA sequence of centromeric heterochromatin is an effective molecular cytogenetic marker for investigating genomic compartmentalization between macrochromosomes and microchromosomes in birds. We isolated four repetitive sequence families of centromeric heterochromatin from three Anseriformes species, viz., domestic duck (Anas platyrhynchos, APL), bean goose (Anser fabalis, AFA), and whooper swan (Cygnus cygnus, CCY), and characterized the sequences by molecular cytogenetic approach. The 190-bp APL-HaeIII and 101-bp AFA-HinfI-S sequences were localized in almost all chromosomes of A. platyrhynchos and A. fabalis, respectively. However, the 192-bp AFA-HinfI-L and 290-bp CCY-ApaI sequences were distributed in almost all microchromosomes of A. fabalis and in approximately 10 microchromosomes of C. cygnus, respectively. APL-HaeIII, AFA-HinfI-L, and CCY-ApaI showed partial sequence homology with the chicken nuclear-membrane-associated (CNM) repeat families, which were localized primarily to the centromeric regions of microchromosomes in Galliformes, suggesting that ancestral sequences of the CNM repeat families are observed in the common ancestors of Anseriformes and Galliformes. These results collectively provide the possibility that homogenization of centromeric heterochromatin occurred between microchromosomes in Anseriformes and Galliformes; however, homogenization between macrochromosomes and microchromosomes also occurred in some centromeric repetitive sequences. Article in Journal/Newspaper Anser fabalis Cygnus cygnus Whooper Swan Directory of Open Access Journals: DOAJ Articles PLOS ONE 14 3 e0214028 |
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Medicine R Science Q |
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Medicine R Science Q Yoshinobu Uno Chizuko Nishida Ayano Hata Satoshi Ishishita Yoichi Matsuda Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
topic_facet |
Medicine R Science Q |
description |
The highly repetitive DNA sequence of centromeric heterochromatin is an effective molecular cytogenetic marker for investigating genomic compartmentalization between macrochromosomes and microchromosomes in birds. We isolated four repetitive sequence families of centromeric heterochromatin from three Anseriformes species, viz., domestic duck (Anas platyrhynchos, APL), bean goose (Anser fabalis, AFA), and whooper swan (Cygnus cygnus, CCY), and characterized the sequences by molecular cytogenetic approach. The 190-bp APL-HaeIII and 101-bp AFA-HinfI-S sequences were localized in almost all chromosomes of A. platyrhynchos and A. fabalis, respectively. However, the 192-bp AFA-HinfI-L and 290-bp CCY-ApaI sequences were distributed in almost all microchromosomes of A. fabalis and in approximately 10 microchromosomes of C. cygnus, respectively. APL-HaeIII, AFA-HinfI-L, and CCY-ApaI showed partial sequence homology with the chicken nuclear-membrane-associated (CNM) repeat families, which were localized primarily to the centromeric regions of microchromosomes in Galliformes, suggesting that ancestral sequences of the CNM repeat families are observed in the common ancestors of Anseriformes and Galliformes. These results collectively provide the possibility that homogenization of centromeric heterochromatin occurred between microchromosomes in Anseriformes and Galliformes; however, homogenization between macrochromosomes and microchromosomes also occurred in some centromeric repetitive sequences. |
format |
Article in Journal/Newspaper |
author |
Yoshinobu Uno Chizuko Nishida Ayano Hata Satoshi Ishishita Yoichi Matsuda |
author_facet |
Yoshinobu Uno Chizuko Nishida Ayano Hata Satoshi Ishishita Yoichi Matsuda |
author_sort |
Yoshinobu Uno |
title |
Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
title_short |
Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
title_full |
Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
title_fullStr |
Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
title_full_unstemmed |
Molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species. |
title_sort |
molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three anseriformes species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0214028 https://doaj.org/article/50020f8da050445eba8bc5b0cf1dfb32 |
genre |
Anser fabalis Cygnus cygnus Whooper Swan |
genre_facet |
Anser fabalis Cygnus cygnus Whooper Swan |
op_source |
PLoS ONE, Vol 14, Iss 3, p e0214028 (2019) |
op_relation |
https://doi.org/10.1371/journal.pone.0214028 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0214028 https://doaj.org/article/50020f8da050445eba8bc5b0cf1dfb32 |
op_doi |
https://doi.org/10.1371/journal.pone.0214028 |
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PLOS ONE |
container_volume |
14 |
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3 |
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e0214028 |
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1766005815450271744 |