Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children
Abstract INTRODUCTION: Methicillin resistant Staphylococcus hominis (MRSHo) has been recognized as an important human pathogen, particularly in immunocompromised patients. METHODS: A total of 19 S. hominis isolates were collected from children at the Children’s Medical Centre, Tehran, Iran, from Mar...
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ftdoajarticles:oai:doaj.org/article:4fc9302beb674f1f9619a2ae5d4e4f1f 2023-05-15T15:07:29+02:00 Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children Setareh Soroush Fereshteh Jabalameli Morovat Taherikalani Mohammad Amin Eslampour Reza Beigverdi Mohammad Emaneini https://doi.org/10.1590/0037-8682-0384-2016 https://doaj.org/article/4fc9302beb674f1f9619a2ae5d4e4f1f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017002300329&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0384-2016 https://doaj.org/article/4fc9302beb674f1f9619a2ae5d4e4f1f Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 3, Pp 329-333 MRSHo SCCmec ACME Biofilm Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0384-2016 2022-12-30T23:24:11Z Abstract INTRODUCTION: Methicillin resistant Staphylococcus hominis (MRSHo) has been recognized as an important human pathogen, particularly in immunocompromised patients. METHODS: A total of 19 S. hominis isolates were collected from children at the Children’s Medical Centre, Tehran, Iran, from March 2012 to February 2013. MRSHo susceptibility against 13 antimicrobial and 3 antiseptic agents was determined using disk diffusion (DAD) and minimum inhibitory concentration (MIC), respectively. All isolates were subjected to polymerase chain reaction (PCR) assay for 15 distinct resistance genes, staphylococcal cassette chromosome mec (SCCmec), and arginine catabolic mobile elements (ACMEs). Biofilm production of the isolates was determined using a colorimetric microtiter plate assay. RESULTS: Of the 19 isolates, 16 were resistant to oxacillin and harbored mecA. High resistance was also observed against trimethoprim/sulfamethoxazole (81.2%). All MRSHo isolates were susceptible to the three disinfectants tested (Septicidine-PC, Septi turbo, and Sayacept-HP). In total, 15 (78.9%) isolates produced biofilms. Three isolates had SCCmec types (V and VIII), 13 were untypable (UT), and 5 had ACME type II. CONCLUSIONS: The results indicate that MRSHo with high antibiotic resistance and unknown SCCmec might become a serious problem in the future for the treatment of patients such as children. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 50 3 329 333 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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MRSHo SCCmec ACME Biofilm Arctic medicine. Tropical medicine RC955-962 |
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MRSHo SCCmec ACME Biofilm Arctic medicine. Tropical medicine RC955-962 Setareh Soroush Fereshteh Jabalameli Morovat Taherikalani Mohammad Amin Eslampour Reza Beigverdi Mohammad Emaneini Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
topic_facet |
MRSHo SCCmec ACME Biofilm Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Methicillin resistant Staphylococcus hominis (MRSHo) has been recognized as an important human pathogen, particularly in immunocompromised patients. METHODS: A total of 19 S. hominis isolates were collected from children at the Children’s Medical Centre, Tehran, Iran, from March 2012 to February 2013. MRSHo susceptibility against 13 antimicrobial and 3 antiseptic agents was determined using disk diffusion (DAD) and minimum inhibitory concentration (MIC), respectively. All isolates were subjected to polymerase chain reaction (PCR) assay for 15 distinct resistance genes, staphylococcal cassette chromosome mec (SCCmec), and arginine catabolic mobile elements (ACMEs). Biofilm production of the isolates was determined using a colorimetric microtiter plate assay. RESULTS: Of the 19 isolates, 16 were resistant to oxacillin and harbored mecA. High resistance was also observed against trimethoprim/sulfamethoxazole (81.2%). All MRSHo isolates were susceptible to the three disinfectants tested (Septicidine-PC, Septi turbo, and Sayacept-HP). In total, 15 (78.9%) isolates produced biofilms. Three isolates had SCCmec types (V and VIII), 13 were untypable (UT), and 5 had ACME type II. CONCLUSIONS: The results indicate that MRSHo with high antibiotic resistance and unknown SCCmec might become a serious problem in the future for the treatment of patients such as children. |
format |
Article in Journal/Newspaper |
author |
Setareh Soroush Fereshteh Jabalameli Morovat Taherikalani Mohammad Amin Eslampour Reza Beigverdi Mohammad Emaneini |
author_facet |
Setareh Soroush Fereshteh Jabalameli Morovat Taherikalani Mohammad Amin Eslampour Reza Beigverdi Mohammad Emaneini |
author_sort |
Setareh Soroush |
title |
Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
title_short |
Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
title_full |
Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
title_fullStr |
Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
title_full_unstemmed |
Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children |
title_sort |
characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant staphylococcus hominis from blood cultures of children |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0384-2016 https://doaj.org/article/4fc9302beb674f1f9619a2ae5d4e4f1f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 3, Pp 329-333 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017002300329&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0384-2016 https://doaj.org/article/4fc9302beb674f1f9619a2ae5d4e4f1f |
op_doi |
https://doi.org/10.1590/0037-8682-0384-2016 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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50 |
container_issue |
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329 |
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333 |
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