Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction
Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (±1.07% SD) and 56.78% (±1.22% SD) (dry weight basis), respectively....
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ftdoajarticles:oai:doaj.org/article:4faa37d0f6274f8cb347cdd6f449b11e 2023-05-15T16:08:54+02:00 Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction Haiyan Ju Xiuying Liu Gang Zhang Dezheng Liu Yongsheng Yang 2020-01-01T00:00:00Z https://doi.org/10.3390/ma13020358 https://doaj.org/article/4faa37d0f6274f8cb347cdd6f449b11e EN eng MDPI AG https://www.mdpi.com/1996-1944/13/2/358 https://doaj.org/toc/1996-1944 1996-1944 doi:10.3390/ma13020358 https://doaj.org/article/4faa37d0f6274f8cb347cdd6f449b11e Materials, Vol 13, Iss 2, p 358 (2020) type i collagen fibrils acid-solubilized method pepsin-aided extraction aggregation structure morphology Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 article 2020 ftdoajarticles https://doi.org/10.3390/ma13020358 2022-12-31T11:37:33Z Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (±1.07% SD) and 56.78% (±1.22% SD) (dry weight basis), respectively. The analyses of both amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that A-CF and P-CF were type I collagen fibrils. Both A-CF and P-CF retained the intact crystallinity and integrity of type I collagen’s natural structure by FTIR spectra, circular dichroism spectroscopy (CD) and X-ray diffraction detection. The aggregation structures of A-CF and P-CF were displayed by UV−Vis. However, A-CF showed more intact aggregation structure than P-CF. Microstructure and D-periodicities of A-CF and P-CF were observed (SEM and TEM). The diameters of A-CF and P-CF are about 386 and 282 nm, respectively. Although both A-CF and P-CF were theoretically concordant with the Schmitt hypothesis, A-CF was of evener thickness and higher integrity in terms of aggregation structure than P-CF. Modified acid-solubilized method provides a potential non-enzyme alternative to extract native collagen fibrils with uniform thickness and integral aggregation structure. Article in Journal/Newspaper evener Directory of Open Access Journals: DOAJ Articles Materials 13 2 358 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
type i collagen fibrils acid-solubilized method pepsin-aided extraction aggregation structure morphology Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 |
spellingShingle |
type i collagen fibrils acid-solubilized method pepsin-aided extraction aggregation structure morphology Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Haiyan Ju Xiuying Liu Gang Zhang Dezheng Liu Yongsheng Yang Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
topic_facet |
type i collagen fibrils acid-solubilized method pepsin-aided extraction aggregation structure morphology Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 |
description |
Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (±1.07% SD) and 56.78% (±1.22% SD) (dry weight basis), respectively. The analyses of both amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that A-CF and P-CF were type I collagen fibrils. Both A-CF and P-CF retained the intact crystallinity and integrity of type I collagen’s natural structure by FTIR spectra, circular dichroism spectroscopy (CD) and X-ray diffraction detection. The aggregation structures of A-CF and P-CF were displayed by UV−Vis. However, A-CF showed more intact aggregation structure than P-CF. Microstructure and D-periodicities of A-CF and P-CF were observed (SEM and TEM). The diameters of A-CF and P-CF are about 386 and 282 nm, respectively. Although both A-CF and P-CF were theoretically concordant with the Schmitt hypothesis, A-CF was of evener thickness and higher integrity in terms of aggregation structure than P-CF. Modified acid-solubilized method provides a potential non-enzyme alternative to extract native collagen fibrils with uniform thickness and integral aggregation structure. |
format |
Article in Journal/Newspaper |
author |
Haiyan Ju Xiuying Liu Gang Zhang Dezheng Liu Yongsheng Yang |
author_facet |
Haiyan Ju Xiuying Liu Gang Zhang Dezheng Liu Yongsheng Yang |
author_sort |
Haiyan Ju |
title |
Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
title_short |
Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
title_full |
Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
title_fullStr |
Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
title_full_unstemmed |
Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction |
title_sort |
comparison of the structural characteristics of native collagen fibrils derived from bovine tendons using two different methods: modified acid-solubilized and pepsin-aided extraction |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ma13020358 https://doaj.org/article/4faa37d0f6274f8cb347cdd6f449b11e |
genre |
evener |
genre_facet |
evener |
op_source |
Materials, Vol 13, Iss 2, p 358 (2020) |
op_relation |
https://www.mdpi.com/1996-1944/13/2/358 https://doaj.org/toc/1996-1944 1996-1944 doi:10.3390/ma13020358 https://doaj.org/article/4faa37d0f6274f8cb347cdd6f449b11e |
op_doi |
https://doi.org/10.3390/ma13020358 |
container_title |
Materials |
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13 |
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2 |
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358 |
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1766404915698073600 |