Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence.
Background Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-res...
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ftdoajarticles:oai:doaj.org/article:4f8bc775e33845b491b7265aab1f301a 2023-05-15T15:09:27+02:00 Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López 2019-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007547 https://doaj.org/article/4f8bc775e33845b491b7265aab1f301a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007547 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007547 https://doaj.org/article/4f8bc775e33845b491b7265aab1f301a PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007547 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007547 2022-12-31T10:59:43Z Background Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-restricted immune response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe chronic polyarthralgia and polyarthritis, is largely unknown. Methodology/principal findings In this study, a high-throughput mass spectrometry analysis of complex HLA-bound peptide pools isolated from large amounts of human cells infected with a vaccinia virus (VACV) recombinant expressing CHIKV structural proteins was carried out. Twelve viral ligands from the CHIKV polyprotein naturally presented by different HLA-A, -B, and -C class I, and HLA-DR and -DP class II molecules were identified. Conclusions/significance The immunoprevalence of the HLA class II but not the HLA class I-restricted cellular immune response against the CHIKV structural polyprotein was greater than that against the VACV vector itself. In addition, most of the CHIKV HLA class I and II ligands detected by mass spectrometry are not conserved compared to its closely related O'nyong-nyong virus. These findings have clear implications for analysis of both cytotoxic and helper immune responses against CHIKV as well as for the future studies focused in the exacerbated T helper response linked to chronic musculoskeletal disorders in CHIKV patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 7 e0007547 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-restricted immune response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe chronic polyarthralgia and polyarthritis, is largely unknown. Methodology/principal findings In this study, a high-throughput mass spectrometry analysis of complex HLA-bound peptide pools isolated from large amounts of human cells infected with a vaccinia virus (VACV) recombinant expressing CHIKV structural proteins was carried out. Twelve viral ligands from the CHIKV polyprotein naturally presented by different HLA-A, -B, and -C class I, and HLA-DR and -DP class II molecules were identified. Conclusions/significance The immunoprevalence of the HLA class II but not the HLA class I-restricted cellular immune response against the CHIKV structural polyprotein was greater than that against the VACV vector itself. In addition, most of the CHIKV HLA class I and II ligands detected by mass spectrometry are not conserved compared to its closely related O'nyong-nyong virus. These findings have clear implications for analysis of both cytotoxic and helper immune responses against CHIKV as well as for the future studies focused in the exacerbated T helper response linked to chronic musculoskeletal disorders in CHIKV patients. |
format |
Article in Journal/Newspaper |
author |
Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López |
author_facet |
Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López |
author_sort |
Elena Lorente |
title |
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
title_short |
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
title_full |
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
title_fullStr |
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
title_full_unstemmed |
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
title_sort |
immunoproteomic analysis of a chikungunya poxvirus-based vaccine reveals high hla class ii immunoprevalence. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007547 https://doaj.org/article/4f8bc775e33845b491b7265aab1f301a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007547 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007547 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007547 https://doaj.org/article/4f8bc775e33845b491b7265aab1f301a |
op_doi |
https://doi.org/10.1371/journal.pntd.0007547 |
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PLOS Neglected Tropical Diseases |
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13 |
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7 |
container_start_page |
e0007547 |
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1766340644843814912 |